LOCUS       Exported                 900 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pRL-TK let7 C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Doench JG, Sharp PA
  TITLE     Specificity of microRNA target selection in translational 
            repression.
  JOURNAL   Genes Dev. 2004 Mar 1. 18(5):504-11.
  PUBMED    15014042
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    280..401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(291..310)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     345..364
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        534..638
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(856..875)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cagagtttcc taatctggaa tttgtcaagt aaaaggtctt catttttcgc aagaagatgc
       61 acctgatgaa atgggaaaat atatcaaatc gttcgttgag cgagttctca aaaatgaaca
      121 ataattctag aaagttttca caaagctaac aggtacctcg agaactatac aacgtctact
      181 caccggaact atacaacgtc tactcaacta gtgtaccaag ttttcacaaa gctaacaccg
      241 gatcgcgggc cctagagcgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
      301 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
      361 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
      421 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
      481 acaaatgtgg taaaatcgat aaggatccag gtggcacttt tcggggaaat gtgcgcggaa
      541 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      601 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      661 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      721 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      781 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccatgatgag
      841 cacnttttaa gtnctgctat gtggcgcggt attatccngt attgacgcgg cagacaccgc
//
