LOCUS Exported 12108 bp ds-DNA circular SYN 27-JUL-2022 DEFINITION Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).. ACCESSION . VERSION . KEYWORDS pC-G418-YR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12108) AUTHORS Sidhu YS, Cairns TC, Chaudhari YK, Usher J, Talbot NJ, Studholme DJ, Csukai M, Haynes K TITLE Exploitation of sulfonylurea resistance marker and non-homologous end joining mutants for functional analysis in Zymoseptoria tritici. JOURNAL Fungal Genet Biol. 2015 Jun;79:102-9. doi: 10.1016/j.fgb.2015.04.015. PUBMED 26092796 REFERENCE 2 (bases 1 to 12108) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 27, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12108 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..25 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS 508..1302 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVGDRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(562..581) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 1172..1191 /label=Neo-F /note="Neomycin resistance gene, forward primer" CDS 1674..1691 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" terminator 1726..1978 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" misc_feature 2000..2024 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 3325..3954 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 4383..5456 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 5522..5716 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(5952..5974) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 6060..6200 /label=bom /note="basis of mobility region from pBR322" primer_bind 6115..6134 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(6215..6232) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6386..6974) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6466..6485) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8399..9193) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" CDS complement(9380..10183) /codon_start=1 /gene="S. cerevisiae URA3" /product="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /label=URA3 /note="yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" promoter complement(10184..10399) /gene="S. cerevisiae URA3" /label=URA3 promoter ORIGIN 1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg 61 gacgttttta atgtactgaa ttaacgccga attaattcct aggccaccat gttgggcccg 121 gcgcgccgaa ttcgagctcg gtacccaact gatattgaag gagcattttt tgggcttggc 181 tggagctagt ggaggtcaac aatgaatgcc tattttggtt tagtcgtcca ggcggtgagc 241 acaaaatttg tgtcgtttga caagatggtt catttaggca actggtcaga tcagccccac 301 ttgtagcagt agcggcggcg ctcgaagtgt gactcttatt agcagacagg aacgaggaca 361 ttattatcat ctgctgcttg gtgcacgata acttggtgcg tttgtcaagc aaggtaagtg 421 gacgacccgg tcataccttc ttaagttcgc ccttcctccc tttatttcag attcaatctg 481 acttacctat tctacccaag catccaaatg attgaacaag atggattgca cgcaggttct 541 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc 601 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc 661 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc 721 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg 781 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag 841 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc 901 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt 961 cttgtcgatc aggatgatct ggacgaagaa catcaggggc tcgcgccagc cgaactgttc 1021 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc 1081 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg 1141 ctgggtgtgg gggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagaa 1201 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg 1261 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaatcattcc actcaacatt 1321 caggctcctc tgcgcacgta aagtgccaaa ggcaataccc tgctcggtgg aatgccgccg 1381 ggcttgtcga ttttacgcac atatgcgcat tcttgacttg aagcggagga gttcttcgtt 1441 gcgggttaca gtgttttaat aaaagaatgg tcaaatcaaa ctgctagata tacctgtcag 1501 acactctagt tgttgacccc tatactctta atacatcaga cagtacatgc atgttgcatg 1561 atgatagtag atgccgaccg ggaaccagtt ggggatcctc tagagtcgac ctgcaggcat 1621 gcaagcttta agaggagtcc accatggtag atctgactag tgttaacgct agccaccacc 1681 accaccacca cgtgtgaatt acaggtgacc agctcgaatt tccccgatcg ttcaaacatt 1741 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa 1801 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg 1861 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa 1921 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg 1981 gaattaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg 2041 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg 2101 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc 2161 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt 2221 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg 2281 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca 2341 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg 2401 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga 2461 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc 2521 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac 2581 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt 2641 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg 2701 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc 2761 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg 2821 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc 2881 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc 2941 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga 3001 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt 3061 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc 3121 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc 3181 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg 3241 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa 3301 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg 3361 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat 3421 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa 3481 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc 3541 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg 3601 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata 3661 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg 3721 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt 3781 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg 3841 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac 3901 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat 3961 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac 4021 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca 4081 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca 4141 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat 4201 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa 4261 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta 4321 agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg 4381 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc 4441 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac 4501 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc 4561 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt 4621 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg 4681 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag 4741 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc 4801 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg 4861 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc 4921 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca 4981 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg 5041 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt 5101 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg 5161 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct 5221 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct 5281 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga 5341 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga 5401 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta 5461 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc 5521 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca 5581 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag 5641 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc 5701 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta 5761 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg 5821 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc 5881 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat 5941 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 6001 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 6061 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat 6121 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa 6181 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 6241 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 6301 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 6361 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 6421 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 6481 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 6541 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 6601 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 6661 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 6721 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 6781 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 6841 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 6901 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 6961 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 7021 aaaactcacg ttaagggctg atgaatcccc taatgatttt ggtaaaaatc attaagttaa 7081 ggtggataca catcttgtca tatgatcaaa tggtttcgcg aaaaatcaat aatcagacaa 7141 caagatgtgc gaactcgata ttttacacga ctctctttac caattctgcc ccgaattaca 7201 cttaaaacga ctcaacagct taacgttggc ttgccacgcc ttacttgact gtaaaactct 7261 cactcttacc gaacttggcc gtaacctgcc aaccaaagcg agaacaaaac ataacatcaa 7321 acgaatcgac cgattgttag gtaatcgtca cctccacaaa gagcgactcg ctgtataccg 7381 ttggcatgct agctttatct gttcgggcaa tacgatgccc attgtacttg ttgactggtc 7441 tgatattcgt gagcaaaaac gacttatggt attgcgagct tcagtcgcac tacacggtcg 7501 ttctgttact ctttatgaga aagcgttccc gctttcagag caatgttcaa agaaagctca 7561 tgaccaattt ctagccgacc ttgcgagcat tctaccgagt aacaccacac cgctcattgt 7621 cagtgatgct ggctttaaag tgccatggta taaatccgtt gagaagctgg gttggtactg 7681 gttaagtcga gtaagaggaa aagtacaata tgcagaccta ggagcggaaa actggaaacc 7741 tatcagcaac ttacatgata tgtcatctag tcactcaaag actttaggct ataagaggct 7801 gactaaaagc aatccaatct catgccaaat tctattgtat aaatctcgct ctaaaggccg 7861 aaaaaatcag cgctcgacac ggactcattg tcaccacccg tcacctaaaa tctactcagc 7921 gtcggcaaag gagccatggg ttctagcaac taacttacct gttgaaattc gaacacccaa 7981 acaacttgtt aatatctatt cgaagcgaat gcagattgaa gaaaccttcc gagacttgaa 8041 aagtcctgcc tacggactag gcctacgcca tagccgaacg agcagctcag agcgttttga 8101 tatcatgctg ctaatcgccc tgatgcttca actaacatgt tggcttgcgg gcgttcatgc 8161 tcagaaacaa ggttgggaca agcacttcca ggctaacaca gtcagaaatc gaaacgtact 8221 ctcaacagtt cgcttaggca tggaagtttt gcggcattct ggctacacaa taacaaggga 8281 agacttactc gtggctgcaa ccctactagc tcaaaattta ttcacacatg gttacgcttt 8341 ggggaaatta tgaggggatc tctcagcgtt aagggatttt ggtcatgcat tctaggtact 8401 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca 8461 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga 8521 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc 8581 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga 8641 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa 8701 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt 8761 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga 8821 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt 8881 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc 8941 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata 9001 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca 9061 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc 9121 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc 9181 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga 9241 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata 9301 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga 9361 gccgattttg aaaccgcggt tagttttgct ggccgcatct tctcaaatat gcttcccagc 9421 ctgcttttct gtaacgttca ccctctacct tagcatccct tccctttgca aatagtcctc 9481 ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt ctatactgtt 9541 gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt 9601 aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt 9661 cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg gagcccttgc 9721 atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct tctgccgcct 9781 gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta atgtctgccc 9841 attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag 9901 caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct 9961 taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt agtaaacaaa 10021 ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg 10081 aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac 10141 taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat cttcgtttcc 10201 tgcaggtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat gtttcttcaa 10261 cactacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct 10321 gttcggagat taccgaatca aaaaaatttc aaggaaaccg aaatcaaaaa aaagaataaa 10381 aaaaaaatga tgaattgaaa agctagctta tcgatgataa gctgtcaaac atgagaatta 10441 attccacgga ctatagacta tacctagtat actccgtcta ctgtacgata cacttccgct 10501 caggtccttg tcctttaacg aggccttacc actcttttgt tactctattg atccagctca 10561 gcaaaggcag tgtgatctaa gattctattc gcgatgtagt aaaactagct agaccgagaa 10621 agagactaga aatgcaaaag gcacttctac aatggctgcc atcattatta tccgatgtga 10681 cgctgcagct tctcaatgat attcgaatac gctttgagga gatacagcct aatatccgac 10741 aaactgtttt acagatttac gatcgtactt gttacccatc attgaatttt gaacatccga 10801 acctgggagt tttccctgaa acagatagta tatttgaacc tgtataataa tatatagtct 10861 agcgctttac ggaagacaat gtatgtattt cggttcctgg agaaactatt gcatctattg 10921 cataggtaat cttgcacgtc gcatccccgg ttcattttct gcgtttccat cttgcacttc 10981 aatagcatat ctttgttaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg 11041 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca 11101 acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg 11161 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa 11221 atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa 11281 aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct 11341 ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta 11401 gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc 11461 gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga 11521 ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat 11581 gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg 11641 tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta 11701 caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt 11761 tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga 11821 gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcgccgcgg tgatcacagg 11881 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt 11941 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg 12001 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag 12061 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctgg //