LOCUS       Exported               13709 bp ds-DNA     circular SYN 16-AUG-2022
DEFINITION  Human EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag 
            biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    pHR EGFR-SLP76-TagBFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13709)
  AUTHORS   Farahani PE, Yang X, Mesev E, Fomby K, Bashor C, Nelson CM, 
            Toettcher JE
  TITLE     pYtags enable spatiotemporal measurements of receptor tyrosine 
            kinase signaling in living cells
  JOURNAL   bioRxiv (2022)
REFERENCE   2  (bases 1 to 13709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 16, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13709
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             402..446
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    731..848
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1001..1408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1474..5103
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             5458..6156
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    6254..6842
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6307..6327)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6725..6736)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6845..6861)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6846..6862)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6952..7185
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7210..7228)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7211..7228)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7518..7537)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7751..7770)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7870..7892
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7930..7948)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8016..8120
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8121..8981
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8339..8358)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9152..9740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9641..9660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9894..9911
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9981..10001)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9986..10315
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10166..10301
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10228..10247
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          11449..11514
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11644..11664
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12089..12223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12126..12145)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12180..12199
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             12391..13024
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    13071..13196
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13693..217
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ggttcttggg agcagcagga agcactatag gcgcagcgtc aatgacgctg acggtacagg
       61 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
      121 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
      181 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
      241 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
      301 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
      361 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
      421 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
      481 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
      541 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
      601 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
      661 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccaa atggcagtat
      721 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
      781 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
      841 aaaattttcg ggtttattac agggacagca gagatccagt ttggatcgat aagcttgata
      901 tcgaattcct gcagccccga taaaataaaa gattttattt agtctccaga aaaagggggg
      961 aatgaaagac cccacctgta ggtttggcaa gctagctgca gtaacgccat tttgcaaggc
     1021 atggaaaaat accaaaccaa gaatagagaa gttcagatca agggcgggta catgaaaata
     1081 gctaacgttg ggccaaacag gatatctgcg gtgagcagtt tcggccccgg cccggggcca
     1141 agaacagatg gtcaccgcag tttcggcccc ggcccgaggc caagaacaga tggtccccag
     1201 atatggccca accctcagca gtttcttaag acccatcaga tgtttccagg ctcccccaag
     1261 gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc agcctgcttc tcgcttctgt
     1321 tcgcgcgctt ctgcttcccg agctctataa aagagctcac aacccctcac tcggcgcgcc
     1381 agtcctccga cagactgagt cgcccggggg ggatctggag ctctcgagaa ttctcacgcg
     1441 tcaagtggag caaggcaggt ggacagtgga tccatgcgac cctccgggac ggccggggca
     1501 gcgctcctgg cgctgctggc tgcgctctgc ccggcgagtc gggctctgga ggaaaagaaa
     1561 gtttgccaag gcacgagtaa caagctcacg cagttgggca cttttgaaga tcattttctc
     1621 agcctccaga ggatgttcaa taactgtgag gtggtccttg ggaatttgga aattacctat
     1681 gtgcagagga attatgatct ttccttctta aagaccatcc aggaggtggc tggttatgtc
     1741 ctcattgccc tcaacacagt ggagcgaatt cctttggaaa acctgcagat catcagagga
     1801 aatatgtact acgaaaattc ctatgcctta gcagtcttat ctaactatga tgcaaataaa
     1861 accggactga aagagctgcc catgagaaat ttacaggaaa tcctgcatgg cgccgtgcgg
     1921 ttcagcaaca accctgccct gtgcaacgtg gagagcatcc agtggcggga catagtcagc
     1981 agtgactttc tcagcaacat gtcgatggac ttccagaacc acctgggcag ctgccaaaag
     2041 tgtgatccaa gctgtcccaa tgggagctgc tggggtgcag gagaggagaa ctgccagaaa
     2101 ctgaccaaaa tcatctgtgc ccagcagtgc tccgggcgct gccgtggcaa gtcccccagt
     2161 gactgctgcc acaaccagtg tgctgcaggc tgcacaggcc cccgggagag cgactgcctg
     2221 gtctgccgca aattccgaga cgaagccacg tgcaaggaca cctgcccccc actcatgctc
     2281 tacaacccca ccacgtacca gatggatgtg aaccccgagg gcaaatacag ctttggtgcc
     2341 acctgcgtga agaagtgtcc ccgtaattat gtggtgacag atcacggctc gtgcgtccga
     2401 gcctgtgggg ccgacagcta tgagatggag gaagacggcg tccgcaagtg taagaagtgc
     2461 gaagggcctt gccgcaaagt gtgtaacgga ataggtattg gtgaatttaa agactcactc
     2521 tccataaatg ctacgaatat taaacacttc aaaaactgca cctccatcag tggcgatctc
     2581 cacatcctgc cggtggcatt taggggtgac tccttcacac atactcctcc tctggatcca
     2641 caggaactgg atattctgaa aaccgtaaag gaaatcacag ggtttttgct gattcaggct
     2701 tggcctgaaa acaggacgga cctccatgcc tttgagaacc tagaaatcat acgcggcagg
     2761 accaagcaac atggtcagtt ttctcttgca gtcgtcagcc tgaacataac atccttggga
     2821 ttacgctccc tcaaggagat aagtgatgga gatgtgataa tttcaggaaa caaaaatttg
     2881 tgctatgcaa atacaataaa ctggaaaaaa ctgtttggga cctccggtca gaaaaccaaa
     2941 attataagca acagaggtga aaacagctgc aaggccacag gccaggtctg ccatgccttg
     3001 tgctcccccg agggctgctg gggcccggag cccagggact gcgtctcttg ccggaatgtc
     3061 agccgaggca gggaatgcgt ggacaagtgc aaccttctgg agggtgagcc aagggagttt
     3121 gtggagaact ctgagtgcat acagtgccac ccagagtgcc tgcctcaggc catgaacatc
     3181 acctgcacag gacggggacc agacaactgt atccagtgtg cccactacat tgacggcccc
     3241 cactgcgtca agacctgccc ggcaggagtc atgggagaaa acaacaccct ggtctggaag
     3301 tacgcagacg ccggccatgt gtgccacctg tgccatccaa actgcaccta cggatgcact
     3361 gggccaggtc ttgaaggctg tccaacgaat gggcctaaga tcccgtccat cgccactggg
     3421 atggtggggg ccctcctctt gctgctggtg gtggccctgg ggatcggcct cttcatgcga
     3481 aggcgccaca tcgttcggaa gcgcacgctg cggaggctgc tgcaggagag ggagcttgtg
     3541 gagcctctta cacccagtgg agaagctccc aaccaagctc tcttgaggat cttgaaggaa
     3601 actgaattca aaaagatcaa agtgctgggc tccggtgcgt tcggcacggt gtataaggga
     3661 ctctggatcc cagaaggtga gaaagttaaa attcccgtcg ctatcaagga attaagagaa
     3721 gcaacatctc cgaaagccaa caaggaaatc ctcgatgaag cctacgtgat ggccagcgtg
     3781 gacaaccccc acgtgtgccg cctgctgggc atctgcctca cctccaccgt gcaactcatc
     3841 acgcagctca tgcccttcgg ctgcctcctg gactatgtcc gggaacacaa agacaatatt
     3901 ggctcccagt acctgctcaa ctggtgtgtg cagatcgcaa agggcatgaa ctacttggag
     3961 gaccgtcgct tggtgcaccg cgacctggca gccaggaacg tactggtgaa aacaccgcag
     4021 catgtcaaga tcacagattt tgggctggcc aaactgctgg gtgcggaaga gaaagaatac
     4081 catgcagaag gaggcaaagt gcctatcaag tggatggcat tggaatcaat tttacacaga
     4141 atctataccc accagagtga tgtctggagc tacggggtga ccgtttggga gttgatgacc
     4201 tttggatcca agccatatga cggaatccct gccagcgaga tctcctccat cctggagaaa
     4261 ggagaacgcc tccctcagcc acccatatgt accatcgatg tctacatgat catggtcaag
     4321 tgctggatga tagacgcaga tagtcgccca aagttccgtg agttgatcat cgaattctcc
     4381 aaaatggccc gagaccccca gcgctacctt gtcattcagg gggatgaaag aatgcatttg
     4441 ccaagtccta cagactccaa cttctaccgt gccctgatgg atgaagaaga catggacgac
     4501 gtggtggatg ccgacgagta cctcatccca cagcagggct tcttcagcag cccctccacg
     4561 tcacggactc ccctcctgag ctctctgagt gcaaccagca acaattccac cgtggcttgc
     4621 attgatagaa atgggctgca aagctgtccc atcaaggaag acagcttctt gcagcgatac
     4681 agctcagacc ccacaggcgc cttgactgag gacagcatag acgacacctt cctcccagtg
     4741 cctgaataca taaaccagtc cgttcccaaa aggcccgctg gctctgtgca gaatcctgtc
     4801 tatcacaatc agcctctgaa ccccgcgccc agcagagacc cacactacca ggacccccac
     4861 agcactgcag tgggcaaccc cgagtatctc aacactgtcc agcccacctg tgtcaacagc
     4921 acattcgaca gccctgccca ctgggcccag aaaggcagcc accaaattag cctggacaac
     4981 cctgactacc agcaggactt ctttcccaag gaagccaagc caaatggcat ctttaagggc
     5041 tccacagctg aaaatgcaga atacctaagg gtcgcgccac aaagcagtga atttattgga
     5101 gcaggaagcg gcagcgagga tgatggggac tacgaaagcc ccaacgaaga agaagaagct
     5161 cctgttgaag atgatgctga ttacgagccc cctccaagca acgacgagag cggcggttct
     5221 gggggaggct ccgaagatga cggggattat gaaagcccga atgaagaaga agaagcaccg
     5281 gtcgaagatg atgccgatta tgaaccaccc cccagcaatg acgaggggag tggcggatca
     5341 ggttccggag aggatgacgg tgattatgag agtccaaacg aggaagaaga agctcccgtt
     5401 gaggacgatg cggactatga gcccccacct tctaacgatg aaggatcagg ttcaggcatg
     5461 agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac
     5521 catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg
     5581 agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc
     5641 ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag
     5701 cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg
     5761 ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc
     5821 agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag
     5881 gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc
     5941 ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata tagatccaag
     6001 aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga
     6061 atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac
     6121 tgcgacctcc ctagcaaact ggggcacaag cttaatgcgg ccgcaactcc cacctgcaac
     6181 atgcgtgact gactgaggcc gcgactctag agtcgacctg caggcatgca agcttgatat
     6241 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6301 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6361 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6421 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6481 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     6541 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     6601 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     6661 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     6721 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     6781 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     6841 gcatcgatac cgtcgacctc gagggaatta attcgagctc ggtaccttta agaccaatga
     6901 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6961 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     7021 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7081 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7141 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat
     7201 tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta
     7261 attgtagccg cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact
     7321 gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac
     7381 cttctgagtt tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag
     7441 ggtcatcgct agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     7501 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     7561 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     7621 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     7681 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt
     7741 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     7801 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     7861 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     7921 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     7981 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     8041 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8101 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     8161 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     8221 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8281 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8341 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8401 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8461 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8521 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8581 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8641 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8701 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8761 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8821 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8881 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8941 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     9001 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     9061 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9781 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9841 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     9901 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9961 cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
    10021 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
    10081 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    10141 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
    10201 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
    10261 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
    10321 tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga
    10381 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc
    10441 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
    10501 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
    10561 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
    10621 gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
    10681 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
    10741 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
    10801 tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
    10861 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga
    10921 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
    10981 acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag
    11041 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
    11101 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
    11161 cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
    11221 gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta
    11281 ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc
    11341 gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt
    11401 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
    11461 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
    11521 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
    11581 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
    11641 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
    11701 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
    11761 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
    11821 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
    11881 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
    11941 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
    12001 cacatttgta gaggttttac ttgctttaaa aaacctccca cacccccccc tgaacctgaa
    12061 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    12121 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    12181 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    12241 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
    12301 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
    12361 ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac
    12421 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
    12481 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
    12541 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
    12601 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
    12661 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
    12721 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
    12781 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
    12841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
    12901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
    12961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    13021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
    13081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
    13141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
    13201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
    13261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
    13321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
    13381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
    13441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
    13501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg
    13561 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
    13621 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
    13681 agcagtggga ataggagctt tgttccttg
//
