LOCUS Exported 12503 bp ds-DNA circular SYN 16-AUG-2022 DEFINITION Human FGFR1 labeled with CD3-epsilon ITAMs for monitoring with CD3-epsilon/ZtSH2 pYtag biosensor. ACCESSION . VERSION . KEYWORDS pHR FGFR1-CD3-epsilon-FusionRed SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12503) AUTHORS Farahani PE, Yang X, Mesev E, Fomby K, Bashor C, Nelson CM, Toettcher JE TITLE pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells JOURNAL bioRxiv (2022) REFERENCE 2 (bases 1 to 12503) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 16, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12503 /organism="synthetic DNA construct" /mol_type="other DNA" intron 1173..1238 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 1368..1388 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 1813..1947 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1850..1869) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1904..1923 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 2115..2748 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 2795..2920 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3417..3650 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 3835..3879 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 4164..4281 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 4434..4841 /label=SFFV promoter /note="spleen focus-forming virus long terminal repeat (LTR) promoter" CDS complement(5286..5303) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 7676..8371 /codon_start=1 /product="monomeric red fluorescent protein optimized for use as a fusion tag (Shemiakina et al., 2012)" /label=FusionRed /note="mammalian codon-optimized" /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY STGGAGDGGK" misc_feature 8481..9069 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8534..8554) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8952..8963) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(9072..9088) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9073..9089) /label=pBluescriptKS /note="For pBluescript vector" LTR 9179..9412 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" promoter complement(9437..9455) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(9438..9455) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(9745..9764) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(9978..9997) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 10097..10119 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(10157..10175) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 10243..10347 /gene="bla" /label=AmpR promoter CDS 10348..11208 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10566..10585) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 11379..11967 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11868..11887 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 12121..12138 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(12208..12228) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 12213..39 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 12393..25 /label=SV40 ori /note="SV40 origin of replication" primer_bind 12455..12474 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" ORIGIN 1 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc 61 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa 121 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca 181 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat 241 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag 301 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta 361 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc 421 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc 481 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt 541 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg 601 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg 661 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg 721 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc 781 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc 841 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc 901 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg 961 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc 1021 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat 1081 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg 1141 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat 1201 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg 1261 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc 1321 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga 1381 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg 1441 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac 1501 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt 1561 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata 1621 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat 1681 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt 1741 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca 1801 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 1861 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 1921 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc 1981 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa 2041 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact 2101 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct 2161 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg 2221 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa 2281 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg 2341 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca 2401 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta 2461 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg 2521 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt 2581 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2641 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2701 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2761 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc 2821 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac 2881 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat 2941 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa 3001 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga 3061 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc 3121 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat 3181 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa 3241 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg 3301 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 3361 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 3421 gctttgttcc ttgggttctt gggagcagca ggaagcacta taggcgcagc gtcaatgacg 3481 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 3541 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 3601 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 3661 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 3721 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 3781 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 3841 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 3901 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 3961 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 4021 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 4081 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc 4141 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca 4201 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa 4261 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggatc 4321 gataagcttg atatcgaatt cctgcagccc cgataaaata aaagatttta tttagtctcc 4381 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct gcagtaacgc 4441 cattttgcaa ggcatggaaa aataccaaac caagaataga gaagttcaga tcaagggcgg 4501 gtacatgaaa atagctaacg ttgggccaaa caggatatct gcggtgagca gtttcggccc 4561 cggcccgggg ccaagaacag atggtcaccg cagtttcggc cccggcccga ggccaagaac 4621 agatggtccc cagatatggc ccaaccctca gcagtttctt aagacccatc agatgtttcc 4681 aggctccccc aaggacctga aatgaccctg cgccttattt gaattaacca atcagcctgc 4741 ttctcgcttc tgttcgcgcg cttctgcttc ccgagctcta taaaagagct cacaacccct 4801 cactcggcgc gccagtcctc cgacagactg agtcgcccgg gggggatctg gagctctcga 4861 gaattctcac gcgtcaagtg gagcaaggca ggtggacagt ggatccatgt ggagctggaa 4921 gtgcctcctc ttctgggctg tgctggtcac agccacactc tgcaccgcta ggccgtcccc 4981 gaccttgcct gaacaagccc agccctgggg agcccctgtg gaagtggagt ccttcctggt 5041 ccaccccggt gacctgctgc agcttcgctg tcggctgcgg gacgatgtgc agagcatcaa 5101 ctggctgcgg gacggggtgc agctggcgga aagcaaccgc acccgcatca caggggagga 5161 ggtggaggtg caggactccg tgcccgcaga ctccggcctc tatgcttgcg taaccagcag 5221 cccctcgggc agtgacacca cctacttctc cgtcaatgtt tcagatgctc tcccctcctc 5281 ggaggatgat gatgatgatg atgactcctc ttcagaggag aaagaaacag ataacaccaa 5341 accaaaccgt atgcccgtag ctccatattg gacatcccca gaaaagatgg aaaagaaatt 5401 gcatgcagtg ccggctgcca agacagtgaa gttcaaatgc ccttccagtg ggaccccaaa 5461 ccccacactg cgctggttga aaaatggcaa agaattcaaa cctgaccaca gaattggagg 5521 ctacaaggtc cgttatgcca cctggagcat cataatggac tctgtggtgc cctctgacaa 5581 gggcaactac acctgcattg tggagaatga gtacggcagc atcaaccaca cataccagct 5641 ggatgtcgtg gagcggtccc ctcaccggcc catcctgcaa gcagggttgc ccgccaacaa 5701 aacagtggcc ctgggtagca acgtggagtt catgtgtaag gtgtacagtg acccgcagcc 5761 gcacatccag tggctaaagc acatcgaggt gaatgggagc aagattggcc cagacaacct 5821 gccttatgtc cagatcttga agactgctgg agttaatacc accgacaaag agatggaggt 5881 gcttcactta agaaatgtct cctttgagga cgcaggggag tatacgtgct tggcgggtaa 5941 ctctatcgga ctctcccatc actctgcatg gttgaccgtt ctggaagccc tggaagagag 6001 gccggcagtg atgacctcgc ccctgtacct ggagatcatc atctattgca caggggcctt 6061 cctcatctcc tgcatggtgg ggtcggtcat cgtctacaag atgaagagtg gtaccaagaa 6121 gagtgacttc cacagccaga tggctgtgca caagctggcc aagagcatcc ctctgcgcag 6181 acaggtgtct gctgactcca gtgcatccat gaactctggg gttcttctgg ttcggccatc 6241 acggctctcc tccagtggga ctcccatgct agcaggggtc tctgagtatg agcttcccga 6301 agaccctcgc tgggagctgc ctcgggacag actggtctta ggcaaacccc tgggagaggg 6361 ctgctttggg caggtggtgt tggcagaggc tatcgggctg gacaaggaca aacccaaccg 6421 tgtgaccaaa gtggctgtga agatgttgaa gtcggacgca acagagaaag acttgtcaga 6481 cctgatctca gaaatggaga tgatgaagat gatcgggaag cataagaata tcatcaacct 6541 gctgggggcc tgcacgcagg atggtccctt gtatgtcatc gtggagtatg cctccaaggg 6601 caacctgcgg gagtacctgc aggcccggag gcccccaggg ctggaatact gctacaaccc 6661 cagccacaac ccagaggagc agctctcctc caaggacctg gtgtcctgcg cctaccaggt 6721 ggcccgaggc atggagtatc tggcctccaa gaagtgcata caccgagacc tggcagccag 6781 gaatgtcctg gtgacagagg acaatgtgat gaagatagca gactttggcc tcgcacggga 6841 cattcaccac atcgactact ataaaaagac aaccaacggc cgactgcctg tgaagtggat 6901 ggcacccgag gcattatttg accggatcta cacccaccag agtgatgtgt ggtctttcgg 6961 ggtgctcctg tgggagatct tcactctggg cggctcccca taccccggtg tgcctgtgga 7021 ggaacttttc aagctgctga aggagggtca ccgcatggac aagcccagta actgcaccaa 7081 cgagctgtac atgatgatgc gggactgctg gcatgcagtg ccctcacaga gacccacctt 7141 caagcagctg gtggaagacc tggaccgcat cgtggccttg acctccaacc aggagtacct 7201 ggacctgtcc atgcccctgg accagtactc ccccagcttt cccgacaccc ggagctctac 7261 gtgctcctca ggggaggatt ccgtcttctc tcatgagccg ctgcccgagg agccctgcct 7321 gccccgacac ccagcccagc ttgccaatgg cggactcaaa cgccgcggct ctggttctcc 7381 acctcccgtc cctaatcccg attatgagcc cattcggaaa ggtcaaagag atttgtatag 7441 tggcctcaat caaagaagaa tcgggtcagg ttccggcccg ccgccagtcc caaaccccga 7501 ctatgaacca atcaggaaag gccaacggga tttgtactca ggattgaatc agcgcaggat 7561 aggttccggc tccgggccac cacccgtccc taacccagat tacgaaccca tacgcaaggg 7621 acaacgcgat ctttacagcg gattgaacca acgacgaata ggatctggtt ctggcatggt 7681 gagcgagctg attaaggaga acatgcccat gaagctgtac atggagggca ccgtgaacaa 7741 ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat 7801 gagaatcaag gtcgtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag 7861 cttcatgtac ggcagcagaa ccttcatcaa gcaccctccg ggcatccccg acttctttaa 7921 gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt 7981 gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaaggt 8041 tagaggggtg aacttcccag ccaacggccc tgtgatgcag aagaaaacac tcggctggga 8101 ggcctccacc gagacgatgt accccgctga cggcggcctg gaaggcgcat gtgacatggc 8161 cctgaagctc gtgggcgggg gccacctgat ctgcaacctt gagaccacat acagatccaa 8221 gaaacccgct acgaacctca agatgcccgg cgtctacaac gtggaccaca gactggaaag 8281 aatcaaggag gccgacgatg agacctacgt cgagcagcac gaggtggctg tggccagata 8341 ctctactggt ggcgctggtg atggaggtaa agcggccacg cgtgcggccg caactcccac 8401 ctgcaacatg cgtgactgac tgaggccgcg actctagagt cgacctgcag gcatgcaagc 8461 ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg 8521 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 8581 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 8641 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 8701 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 8761 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 8821 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat 8881 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 8941 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 9001 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 9061 cctccccgca tcgataccgt cgacctcgag ggaattaatt cgagctcggt acctttaaga 9121 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 9181 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc 9241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 9301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 9361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag 9421 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta 9481 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg 9541 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg 9601 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa 9661 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg 9721 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg 9781 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg 9841 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca 9901 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc 9961 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac 10021 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga 10081 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa 10141 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 10201 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 10261 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 10321 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 10381 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 10441 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 10501 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 10561 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 10621 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 10681 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 10741 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 10801 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 10861 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 10921 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 10981 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 11041 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 11101 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 11161 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 11221 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 11281 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 11341 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 11401 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 11461 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 11521 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 11581 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 11641 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 11701 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 11761 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 11821 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 11881 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 11941 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 12001 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 12061 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 12121 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 12181 cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc 12241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 12301 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 12361 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 12421 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 12481 cggcctctga gctattccag aag //