LOCUS Exported 11924 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS CX-nestinGFPas SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11924) AUTHORS Eberhardt M, Salmon P, von Mach MA, Hengstler JG, Brulport M, Linscheid P, Seboek D, Oberholzer J, Barbero A, Martin I, Muller B, Trono D, Zulewski H TITLE Multipotential nestin and Isl-1 positive mesenchymal stem cells isolated from human pancreatic islets. JOURNAL Biochem Biophys Res Commun. 2006 Jul 7;345(3):1167-76. Epub 2006 May 11. PUBMED 16713999 REFERENCE 2 (bases 1 to 11924) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11924 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 82..461 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 462..665 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 616..636 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 680..860 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 904..1029 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1526..1759 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1944..1988 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2255..2372 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" primer_bind 2754..2773 /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" polyA_signal 2772..2827 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind 2873..2892 /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" CDS complement(4898..5617) /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS complement(4901..5617) /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(4943..4964) /label=EGFP-C /note="EGFP, forward primer" primer_bind 5292..5311 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5551..5572 /label=EGFP-N /note="EGFP, reverse primer" regulatory 5614..5623 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6148..6177 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 6151..6180 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 6154..6183 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 6157..6186 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 6160..6189 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 6163..6192 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 6166..6195 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 6169..6198 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 6172..6201 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 6175..6204 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 6178..6207 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" primer_bind complement(8347..8363) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8347..8363) /label=pBluescriptSK /note="For pBluescript vector" protein_bind 8532..8565 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." LTR 8585..8765 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" promoter complement(8790..8808) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(8791..8808) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(9066..9085) /label=T7 /note="T7 promoter, forward primer" promoter complement(9067..9085) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9095..9112) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(9095..9111) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9104..9126) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9256..9711 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9343..9362) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9553..9574 /label=F1ori-F /note="F1 origin, forward primer" promoter 9740..9844 /gene="bla" /label=AmpR promoter CDS 9845..10705 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10063..10082) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10876..11464 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11365..11384 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11618..11635 /label=L4440 /note="L4440 vector, forward primer" protein_bind 11752..11773 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11788..11818 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11826..11842 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11831..11853 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 11850..11866 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 11850..11866 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 11885..11905 /label=T3 /note="T3 promoter, forward primer" promoter 11887..11905 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" ORIGIN 1 aagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat 61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta 121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 661 gagctcgttt agtgaaccgg ggtctctctg gttagaccag atctgagcct gggagctctc 721 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 781 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 841 agtgtggaaa atctctagca gtggcgcccg aacagggacc tgaaagcgaa agggaaacca 901 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg 961 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc 1021 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg 1081 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa 1141 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga 1201 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta 1261 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac 1321 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga 1381 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata 1441 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc 1501 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag 1561 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg 1621 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc 1681 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc 1741 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg 1801 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga 1861 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg 1921 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg 1981 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa 2041 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata 2101 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac 2161 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc 2221 gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg 2281 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat 2341 tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgattgcg 2401 gccggccgcg gtacctgggc cccccctcga ggtcgaggtc gagggatctc cataagagaa 2461 gagggacagc tatgactggt gagtagtcag gagaggagga aaaatctggc tagtaaaaca 2521 tgtaaggaaa attttaggga tgttaaagaa aaaaataaca caaaacaaaa tataaaaaaa 2581 atctaacctc aagtcaaggc ttttctatgg aataaggaat ggacagcagg gggctgtttc 2641 atatactgat gacctcttta tagccaacct ttgttcatgg cagccagcat atgggcatat 2701 gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc cctcccatat 2761 gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa aataaatttc 2821 ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata atttttggca 2881 gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc agccaccacc 2941 ttctgatagg cagcctgcac ctgaggagtg aatttcgacg gtatcgataa gctgatcttc 3001 caaggagagc agttggctcc gcctcttacc cagaaagcca agagaagcct gggaacctcg 3061 tccaggtgtc tgcaaccgag agttctcagc ctccagcaga gtcctggtgg ggagtgggga 3121 gatagggtca gctccagctg aggtagcatg tcctgccact gcaggatcaa tctctattgt 3181 gaccattgtc atataaaagc cacacagtca tatacccaca gatatatact tagccaaccc 3241 atatttgaga cacagggaga ccccacatgc agattcccac agtcggaggc agggccaaat 3301 gaattgctaa cacttatatc agactcctca gatcagtctc cgcctcccca cccaaggcca 3361 aggccgatga cctcatcctc tgggagggag gccgattctc atgctaatta ttgccttttg 3421 tccacactac catctggagg gcctaagaag ggagggctcc tcaggggaag tgggaattct 3481 caggctgttc ccaggggatg gctctctctc tgcccccaga gctggtaaca gacaaaagca 3541 aatgaattca gctccccttc tccaaatcct tttcagacct caaacgccag tggttacatt 3601 cctcagagct gcctggaccc ttcccctcag aggactgact ggggctaaag ccctcatctc 3661 aggatcacaa actcttcagg gcagaggtct taacgttcat ggccctgcct cagtggccta 3721 tggagctgat tgaaggagat gagggaaatc agttcacatg ggattcggaa ctagtctgtt 3781 tccattcatg gcctctgagc acatctggtt ctcaaagtac cctttcctct gtcctcatgc 3841 ggcatcctgc cttatgcatt cagtctttcc tagacactca ctggctgtcc ttctctgggc 3901 cctccagctc ctctcctaga cctgtctctg tctttcctct ttaccgctgg attctggaaa 3961 aggggggcat cctgtcctag ccccatactc tggagtgata tcctcagccc tcaaccgtgg 4021 gactccaact ggggcctttt cccacaagag acacagacag aggctgtgaa cataccacca 4081 gagccgttcc actcagccgt cctgaggcag ccaagaaaag ggcagagaac tggggcagga 4141 ccagtgacat gagggcttga aaggaagcaa aaaagctagg aggccagctc ttacccaaaa 4201 cacctgaggc ccaggcgagg tggcaggtta gcatgatcag gcaaacctgc aaggcctctc 4261 ttctgagact gcccaggtgt ccccaggatg ttggcataga cactggagat tgcctgcctt 4321 ccaaagaact catctgtctg gtctcctccc tgaagactga gctgtctttt taagcatcac 4381 cgtctcttct ggtcctgaac ctgggtgggc caagctgcca agccagacct gttccacctc 4441 tgggactcat aagcctccat cccagacaaa tacattacat aagtaaatgt tataaaacgt 4501 tttaagtgac tccgagggct ggacagacgg ctcagtggtt aagaatgctt gctgcttttt 4561 gctgagaaca ggggtttggt tcacagcacc agtgtggagc tgcacacaac ccattgcctt 4621 cctctgggct cttaggacac ctgtacacat gtggtacata cacaaataca cataaatagg 4681 acagttttac aaagcgatag ctgacagtca cagaaagtac aaagccaagt ttctgggccg 4741 cagaagaagc cgaggacagt cagcactgtg tacctgtatg tagccacctc cagactaagg 4801 gacatcttga ggtgtgccag ttgctgccca ccttccagga tcagcttgat atcgaattcc 4861 tgcagcccag cttgcatgcc tgcaggtcga ggccgcttta cttgtacagc tcgtccatgc 4921 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt 4981 tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg 5041 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct 5101 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga 5161 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct 5221 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca 5281 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt 5341 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc 5401 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct 5461 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct 5521 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca 5581 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt ggatctcgac 5641 tggtctggcc cccgagggca agcggacgtg gagcactaga gaagggagtg gcccgtggag 5701 cttttagggg ttgaaaagaa gtagcggcga cagtgaccca gcggaagcgg agaactccag 5761 cagggagctg gagcgagggt attcatactc ccgccggctg ggcggaaaag gggcggcccc 5821 ccaggcctta accctttagc gttcagtgca acacgcaacc ggggttcctg cttcatccgg 5881 gtgcagaaga gaagaaccaa agtgcagccc ctccctgagg gatccggtcc ggtgcgggtg 5941 attttctgag agaatcttga gatacagcca gacaccccgg aacaccctat gatgcacaga 6001 actgggcctg cggacctcct ttctctacac actcaaattc tctgagactc ggcttcttat 6061 ttatagaatg gcttggtgtt ggaatcctca tgaactgttg gaaaaacgga cggtgcagtg 6121 ttttgtgtaa aacagtaaat attcacacac caccaccacc accaccacca ccaccaccac 6181 caccaccacc accaccacca ccaccacctg tccccaggga ggcaacattg cagagagaca 6241 ctgcccgaag caagaaaaac ccactggtac tgggttttcc tgagctccga agcccgaaga 6301 aggattaagg ccagcctggg cagtaaacac cccccacccc tttcttcaga tggttggtat 6361 cgctatgtga atcaggctgg tctcgaaatc gtagagatgc tcctgcttta gcctccctag 6421 tgctgggatt aaaggtctgt gccaacaccc tgcccagcca gcccagttga tgtaacagac 6481 acaatgcaca gttcgtaccc gcagagggac gtcctccccc tcctcatccc gcatcaggaa 6541 aaggaaaatg gcaagggacg tcatacgttc aagaaagttt ttctcctttg ttgttcgtac 6601 actcttgcag tttactcagt tagaaagcta cgcggctctc aggggagccc cctggtggct 6661 aacgatgcag tctggcgaag aaagaaatga agttgtggac tttgtacaag ggacccttag 6721 gcttaaatgt ttgaatgctt ggtccccagt tggagaacct attttagaag gattgggagg 6781 aatggccttg ttggaggagg tgtatcactg ggggtaggct ttgagagagg ttgcagtagc 6841 tcctttctct gcctttgtgc tagtggattg agatgtgagc tctgagtcgc tgctccagtg 6901 ccggtaggcc tgcctgttgc caagctccct cccatgatgt tcaggaccca catagtgagg 6961 agagaactga ctcgcacatg gcatactgta gcatggtgca cctgcgcacg cgcgcacaca 7021 cacacacaca gactaacaac aataacaaca agtaataaat ctaaattctt ttaaataaca 7081 acaagtaata aatctaattc ttttaaatag ctgtttgagt cgataacttg agtgtcatca 7141 aacctcgttc agtgaggcca gttaattcct ttaatcccag cactcaagag acagaataat 7201 gccaatctct gagtttaagg ccagcctcta catagcaaat gagttccagg ccagccagga 7261 ttacatagta agatcctgtc tcaaacgaaa aataaaatcc ttcagtgaat tttggcttac 7321 gattaggaca aaatttccaa caatttctga aatggtccta aacatacttc tgctgtgggc 7381 agaatcattc atacagattg gcattcccag cattgacagt tagaaaatca ggatatctga 7441 gctggagaga tgctaagtcg tcaaaagcac ttgctgctct cccggaggac ccaagtacaa 7501 ttctcagcac ccatatcagc tccagggaca tcaaaggcct ctgacctcta tgtgcatcca 7561 cacacaggta tacatgccca tgcacagaca tacacagaca cacacacaca cacacacaca 7621 cacacacaca cacacacaca gaaatagcaa taaactactt tttctttttc tttttttccg 7681 gagctgggga ccgaacccag ggcctgcgct taataggcaa gcgcttacca ctgagctaaa 7741 tccccaaccc ccataaaact actttttaaa tcaaagtatg gatcaattct gaaaaacgtt 7801 gaagatgttc taccatggct caggaggaac tgagagttca acatctttat ccaaaggaag 7861 ccaggagcag actgtcttcc aggcggctag gaggagggtc ttaaagccca ctcctacagt 7921 gacacacttt ctccaacaag gccacacctc ctaatagtgc cactccctgg gccaagcatt 7981 caaactacca cagtggccca tgcttttagt cccagcaatt aggaggtaga ggcaggcagg 8041 tctctgtgag tttgacacca gcctggccca cttagttcca ggattgccag gactatttag 8101 agagaccctg tctcaaaata aataaataaa tcccacattc ttgactctat atcctttcgg 8161 gagcaggtgg agaaattgct cagatgttaa gagcacactg gctgctcctt cagaggacct 8221 gagtgccaat tcccattgtc cacacggcag ctcacaggtg gcaggaactt cagaggatcc 8281 agtgtccttg tcggccttca ctgcatgcac agacatccac ataggccgaa ttcctgcagc 8341 ccgggggatc cactagttct agagcggcca attcgctcaa gcttcgaatt aattctgcag 8401 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 8461 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tgggatcaat 8521 tcaccatggg aataacttcg tatagcatac attatacgaa gttatgctgc tttttgcttg 8581 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 8641 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 8701 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 8761 tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg 8821 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat 8881 tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga 8941 gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg 9001 gtcagcgaac caatcagcag ggtcatcgct agcctaggct tttgcgtcga gacgtaccca 9061 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg 9121 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca 9181 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga 9241 atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 9301 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 9361 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 9421 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 9481 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 9541 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 9601 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 9661 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcccaggtgg 9721 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 9781 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 9841 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 9901 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 9961 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 10021 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 10081 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 10141 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 10201 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 10261 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 10321 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 10381 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 10441 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 10501 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 10561 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 10621 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 10681 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 10741 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 10801 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 10861 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 10921 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 10981 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta 11041 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 11101 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 11161 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 11221 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 11281 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 11341 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 11401 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 11461 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 11521 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 11581 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 11641 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 11701 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 11761 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 11821 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 11881 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgc //