LOCUS Exported 8648 bp ds-DNA circular SYN 18-AUG-2022 DEFINITION Expression of human 41BBL, CD86 and HLA-A201 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells. ACCESSION . VERSION . KEYWORDS pSBbi 41BBL CD86 HLA-A201 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8648) TITLE McLellan Lab Plasmids 2022 REFERENCE 2 (bases 1 to 8648) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 18, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8648 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(43..60) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 49..273 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" promoter 705..809 /gene="bla" /label=AmpR promoter CDS 810..1670 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1028..1047) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1841..2429 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2330..2349 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2583..2600 /label=L4440 /note="L4440 vector, forward primer" primer_bind 2974..2993 /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" polyA_signal 2992..3047 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind 3093..3112 /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" CDS complement(3812..3868) /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS complement(4865..4921) /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" regulatory 5689..5698 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter 6329..7510 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" primer_bind 7458..7478 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" ORIGIN 1 aggccaagct tggggatcaa ttctctagag ctcgctgatc agcctcgact gtgccttcta 61 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca 121 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc 181 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata 241 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg 301 cctactagat agcggacccc ttaccgaaac atcgccgcat tctgcagagg agtcgaactt 361 agtgtatgta aacttctgac ccactggaat tgtgatacag tgaattataa gtgaaataat 421 ctgtctgtaa acaattgttg gaaaaatgac ttgtgtcatg cacaaagtag atgtcctaac 481 tgacttgcca aaactattgt ttgttaacaa gaaatttgtg gagtagttga aaaacgagtt 541 ttaatgactc caacttaagt gtatgtaaac ttccgacttc aactgtatag ggatccaatt 601 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg 661 cggtatttca caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 721 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 781 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 841 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 901 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 961 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 1021 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 1081 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 1141 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 1201 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 1261 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 1321 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 1381 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 1441 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 1501 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 1561 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 1621 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 1681 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 1741 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 1801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 1861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 1921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 1981 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 2041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 2101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 2161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 2221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 2281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 2341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 2401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 2461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 2521 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 2581 gcagcgagtc agtgagcgag gaagcggatc cctatacagt tgaagtcgga agtttacata 2641 caccttagcc aaatacattt aaactcactt tttcacaatt cctgacattt aatcctagta 2701 aaaattccct gtcttaggtc agttaggatc accactttat tttaagaatg tgaaatatca 2761 gaataatagt agagagaatg attcatttca gcttttattt ctttcatcac attcccagtg 2821 ggtcagaagt ttacatacac tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg 2881 tcccgagtag ctagttcatg gcagccagca tatggcatat gttgccaaac tctaaaccaa 2941 atactcattc tgatgtttta aatgatttgc cctcccatat gtccttccga gtgagagaca 3001 caaaaaattc caacacacta ttgcaatgaa aataaatttc ctttattagc cagaagtcag 3061 atgctcaagg ggcttcatga tgtccccata atttttggca gagggaaaaa gatctcagtg 3121 gtatttgtga gccagggcat tggccacacc agccaccacc ttctgatagg cagcctgcac 3181 ctgaggagtg aattcacgac aggccttcga atcaggcacc gggctttcgg gtcatacacc 3241 atgtgcgcgg tccctcaggg acttcaacat ccgctgtgac ggtaaacccg aggcgctcat 3301 agaaaggcag gtttctcggg gctgaggttt ccaagaacgc tggtacgcct gccctctccg 3361 ctgcctctac accgggcagc actactgcag aaccaagacc tttcccctga tgatctggag 3421 acacgcccac tgttgcaaga aaccacgcag gctccttagg cctgtggggt gccagaagac 3481 cctccatttg ctgctgagcg gccaatcgac ttccgctcaa ttcggccatg cgaggaccga 3541 tctcagcgaa gaccgctccg gcctccactg actcaggagt tgtccagacc gctaccgcag 3601 caccatcatc agcaacccaa acttttccga tatcaagtcc aaccctagtc aaaaacagct 3661 cttgcagctc cgtcactctc tcgatgtgcc tatctgggtc cacagtgtgt cgcgtggccg 3721 gataatcggc gaaagcagca gcaagtgtcc gaaccgccct gggaacatca tcgcgtgttg 3781 ccagtctaac tgtaggcttg tattctgtca tggggccagg attctcctcg acgtcaccag 3841 cctgcttcag caggctgaag ttagtagctc cggatccaaa acatgtatca cttttgtcgc 3901 atgaagatgt cttcgaactt ttaaaaacac gctgggcttc atcagatctt tcaggtatat 3961 ggattttttc tcttttcttg gtctgttcac tctcttccct ctccattgtg ttggttccac 4021 atttataaga gttgcgaggc cgcttcttct tcttccattt ccatagaatt agacagaaaa 4081 ccatcacaca tataataact gttggaagta cagctgtaat ccaaggaatg tggtctgggg 4141 gaggctgagg gtcctcaagc tctatagaga aaggtgaaga taaaagccgc gtcttgtcag 4201 tttccagaat acagaagatg gtcatattgc tcgtaacatc agggaatgaa acagacaagc 4261 tgatggaaac gtcgtacagt tctgtgacat tatcttgaga tttctgcata ataccatcat 4321 actcgatagt tgaattcttg gttcttagca aaacactcat cttcttaggt tctgggtaac 4381 cgtgtataga tgagcaggtc aaatttatgt acacattttc tgttatatta gaaattggta 4441 ctatttcagg ttgactgaag ttagcaagca ctgacagttc agaattcatc tggtggatgc 4501 gaatcattcc tgtgggcttt ttgtgatgga tgatacattg atacaagccc ttgtccttga 4561 tctgaagatt gtgaagtctc agggtccaac tgtccgaatc aaaacttgtg cggcccatat 4621 acttggaatg aacactgtca aatttctctt tgcctaagta tacctcattc agaaccaagt 4681 tttcctggtc ctgccaaaat actactagct cactcaggct ttggttttga gagtttgcaa 4741 attggcatgg caggtctgca gtctcattga aataagcttg aatcttcaga ggagcagcac 4801 cagagagcag gaaggccatc acaaagagaa tgttactcag tcccatagtg cactggggat 4861 ccatggggcc aggattctcc tcgacgtcac cagcctgctt cagcaggctg aagttagtag 4921 ctccggatcc ttccgacctc ggtgaaggga gtccggctgg gatttcgggg gtcacccgga 4981 agagtcccaa gactgtggcg ccctgggtaa gctgccaggc atggcgtgcc ctggcctcag 5041 tgtgaagatg gacgcccagg cgctggccgg cactcaggtg cagcaagcgg ccctggaaac 5101 cgaaggccga gttccgagcc tcggaggagg cgggtggcag gtccacggtc aaagccaggg 5161 cggcggcccc agcagcagag cgcagtggct gcaggtgcag cgcaagtgaa acggagcctg 5221 agccctcgcc ggccaccacg cgccgcagct ctagttgaaa gaagacatag tagactccag 5281 ccttggccac caccagctcc ttcgtgtcct ctttgtagct caggcccccc gtcagggaca 5341 cgcctgccag gcctgggtca ctgtaccagc tcaggggccc atcgatcagc agaacatttt 5401 gggccaccag ctgcgcaaac atgccctgcc gcaggtccaa gaggccggcg ggatcgtcgg 5461 gcgaaagctc gggaccctcg cggagtctcg ggctggccgc ggagccgggc gaggcgcgag 5521 ccccggacac ggcccagggg caggcgagga agacggcgca ggcggcagcg agcagcagca 5581 gcagcagcag ccccgcgacc agggcccaag gcagtacgcg gcaggcgcga gcgcggggcg 5641 cgggaggcca cggggcttcg gggtccagtg aagcgtcaga ggcgtattcc atggtggccg 5701 ccctcaggtg cagaggtttc tacagggaaa gagagaaagg ctacagttag tttgctgtga 5761 tgatgctaag tgccgaggtc agatgtctat ggattgtaca cggaatccca acgcaggaac 5821 caaaaaacac tcacccaaaa aaatggcgct aaggccgaga gaaaggcgaa gttccgtcta 5881 cggctatata ccttgaaaca ggggcggcag aagtgacgca acgcggttgc gcggacggct 5941 tcctgtgttt tcattggtcg aagcgttctg cgcgtgcgca gagattcccc agggaaggcg 6001 ggccctcttc cgaatgtcca atccggacat tcttccggtt ggacctccgc ggtcagcgct 6061 gtgcccggcg agagatcacg tggggcgcgg aggcggtgct gctggggcac ggccgtccag 6121 cctcggcggc catatttttg aggggctgtt catctcgttc acacgctctg tccgccatgt 6181 ttgtgagtgg aagcgccatt accccttcaa gcgactgaag gctgcagggc ctctggtggc 6241 ccgcatgggg agaccagacc cgccaggccc gcctttccgc actcagtccg ggcttacttt 6301 attttgtgag acagggtctc gcctagaggc tccggtgccc gtcagtgggc agagcgcaca 6361 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga 6421 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 6481 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 6541 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg 6601 ggttatggcc cttgcgtgcc ttgaattact tccacgcccc tggctgcagt acgtgattct 6661 tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc 6721 cccttcgcct cgtgcttgag ttgaggcctg gcttgggcgc tggggccgcc gcgtgcgaat 6781 ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt 6841 ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga 6901 tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca 6961 gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta 7021 gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc 7081 ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc 7141 cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga 7201 gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac 7261 ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc 7321 tttaggttgg ggggaggggt tttatgcgat ggagtttccc catactgagt gggtggagac 7381 tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt 7441 tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttaa 7501 ggtgtcgtga aaactacccc aagctggcct ctgaggccca ccatggccgt catggcgccc 7561 cgaaccctcg tcctgctact ctcgggggct ctggccctga cccagacctg ggcgggctct 7621 cactccatga ggtatttctt cacatccgtg tcccggcccg gccgcgggga gccccgcttc 7681 atcgcagtgg gctacgtgga cgacacgcag ttcgtgcggt tcgacagcga cgccgcgagc 7741 cagaggatgg agccgcgggc gccgtggata gagcaggagg gtccggagta ttgggacggg 7801 gagacacgga aagtgaaggc ccactcacag actcaccgag tggacctggg gaccctgcgc 7861 ggctactaca accagagcga ggccggttct cacaccgtcc agaggatgta tggctgcgac 7921 gtggggtcgg actggcgctt cctccgcggg taccaccagt acgcctacga cggcaaggat 7981 tacatcgccc tgaaagagga cctgcgctct tggaccgcgg cggacatggc agctcagacc 8041 accaagcaca agtgggaggc ggcccatgtg gcggagcagt tgagagccta cctggagggc 8101 acgtgcgtgg agtggctccg cagatacctg gagaacggga aggagacgct gcagcgcacg 8161 gacgccccca aaacgcatat gactcaccac gctgtctctg accatgaagc caccctgagg 8221 tgctgggccc tgagcttcta ccctgcggag atcacactga cctggcagcg ggatggggag 8281 gaccagaccc aggacacgga gctcgtggag accaggcctg caggggatgg aaccttccag 8341 aagtgggcgg ctgtggtggt gccttctgga caggagcaga gatacacctg ccatgtgcag 8401 catgagggtt tgcccaagcc cctcaccctg agatgggagc cgtcttccca gcccaccatc 8461 cccatcgtgg gcatcattgc tggcctggtt ctctttggag ctgtgatcac tggagctgtg 8521 gtcgctgctg tgatgtggag gaggaagagc tcagatagaa aaggagggag ctactctcag 8581 gctgcaagca gtgacagtgc ccagggctct gatgtgtctc tcacagcttg taaagtgtga 8641 ggcctgtc //