LOCUS Exported 703 bp ds-DNA circular SYN 18-JAN-2023 DEFINITION evolved sortase A (eSrtA) pentamutant with improved kinetics and activity. ACCESSION . VERSION . KEYWORDS sortase A pentamutant (eSrtA) in pET29 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 703) AUTHORS Chen I, Dorr BM, Liu DR TITLE A general strategy for the evolution of bond-forming enzymes using yeast display. JOURNAL Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11399-404. doi: 10.1073/pnas.1101046108. Epub 2011 Jun 22. PUBMED 21697512 REFERENCE 2 (bases 1 to 703) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 18, 2023 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..703 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(50..69) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 260..281 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 61 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 121 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 181 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 241 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 301 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 361 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag 421 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 481 caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac 541 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat 601 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg 661 attccgactc gtccaacatc aatacaacct attaattctg tct //