LOCUS Exported 11116 bp ds-DNA circular SYN 06-SEP-2022 DEFINITION Gateway destination clone of PKMYT1 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics . ACCESSION . VERSION . KEYWORDS p667-UBC-PKMYT1-V5-TurboID_IDG-K SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11116) TITLE Major lab plasmids for proteomics-based protein-interaction and protein-proximity network generation REFERENCE 2 (bases 1 to 11116) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 6, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11116 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..22 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 37..67 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 75..91 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 80..102 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 99..115 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 99..115 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 134..154 /label=T3 /note="T3 promoter, forward primer" promoter 136..154 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 182..408 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 409..589 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 636..761 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1254..1487 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1672..1716 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 1983..2099 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2131..2151 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" promoter 2151..3362 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 3199..3218 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" protein_bind 3363..3387 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(4885..4909) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 4911..4952 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" CDS 4959..5915 /codon_start=1 /product="mutant of the E. coli biotin ligase BioID engineered for improved catalytic efficiency (Branon et al., 2018)" /label=TurboID /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK" protein_bind complement(5921..5941) /gene="mutant version of attB" /label=attB3 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" misc_feature 5970..6558 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6023..6043) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6441..6452) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6607..6630) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 6991..7010 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 7079..7678 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7079..7098) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7575..7595 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 7816..8049 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 8121..8242 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8158..8177) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8212..8231 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 8282..8417 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8344..8363 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(8437..8456) /label=T7 /note="T7 promoter, forward primer" promoter complement(8438..8456) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8466..8483) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8466..8482) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8475..8497) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 8624..9079 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8711..8730) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8921..8942 /label=F1ori-F /note="F1 origin, forward primer" promoter 9105..9209 /gene="bla" /label=AmpR promoter CDS 9210..10070 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9428..9447) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10241..10829 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10730..10749 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10983..11000 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta 181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat 241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat 301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac 1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca 1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct 1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg 1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg 1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag 1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt 1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa 1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat 1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta 1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta 2101 tcgtccagtg tggtggaatt ctgcagatat caactttgta tagaaaagtt ggcctccgcg 2161 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga 2221 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg 2281 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt 2341 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc 2401 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa 2461 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt 2521 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct 2581 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt 2641 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg 2701 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa 2761 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg 2821 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt 2881 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg 2941 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac 3001 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt 3061 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc 3121 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta 3181 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg 3241 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt 3301 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag 3361 acacaagttt gtacaaaaaa gttggcacca tgctagaacg gcctcctgca ctggccatgc 3421 ccatgcccac ggagggcacc ccgccacctc tgagtggcac ccccatccca gtcccagcct 3481 acttccgcca cgcagaacct ggattctccc tcaagaggcc cagggggctc agccggagcc 3541 tcccacctcc gccccctgcc aagggcagca ttcccatcag ccgcctcttc cctcctcgga 3601 ccccaggctg gcaccagctg cagccccggc gggtgtcatt ccggggcgag gcctcagaga 3661 ctctgcagag ccctgggtat gacccaagcc ggccagagtc cttcttccag cagagcttcc 3721 agaggctcag ccgcctgggc catggctcct acggagaggt cttcaaggtg cgctccaagg 3781 aggacggccg gctctatgcg gtaaagcgtt ccatgtcacc attccggggc cccaaggacc 3841 gggcccgcaa gttggccgag gtgggcagcc acgagaaggt ggggcagcac ccatgctgcg 3901 tgcggctgga gcaggcctgg gaggagggcg gcatcctgta cctgcagacg gagctgtgcg 3961 ggcccagcct gcagcaacac tgtgaggcct ggggtgccag cctgcctgag gcccaggtct 4021 ggggctacct gcgggacacg ctgcttgccc tggcccatct gcacagccag ggcctggtgc 4081 accttgatgt caagcctgcc aacatcttcc tggggccccg gggccgctgc aagctgggtg 4141 acttcggact gctggtggag ctgggtacag caggagctgg tgaggtccag gagggagacc 4201 cccgctacat ggcccccgag ctgctgcagg gctcctatgg gacagcagcg gatgtgttca 4261 gtctgggcct caccatcctg gaagtggcat gcaacatgga gctgccccac ggtggggagg 4321 gctggcagca gctgcgccag ggctacctgc cccctgagtt cactgccggt ctgtcttccg 4381 agctgcgttc tgtccttgtc atgatgctgg agccagaccc caagctgcgg gccacggccg 4441 aggccctgct ggcactgcct gtgttgaggc agccgcgggc ctggggtgtg ctgtggtgca 4501 tggcagcgga ggccctgagc cgagggtggg ccctgtggca ggccctgctt gccctgctct 4561 gctggctctg gcatgggctg gctcaccctg ccagctggct acagcccctg ggcccgccag 4621 ccaccccgcc tggctcacca ccctgcagtt tgctcctgga cagcagcctc tccagcaact 4681 gggatgacga cagcctaggg ccttcactct cccctgaggc tgtcctggcc cggactgtgg 4741 ggagcacctc caccccccgg agcaggtgca cacccaggga tgccctggac ctaagtgaca 4801 tcaactcaga gcctcctcgg ggctccttcc cctcctttga gcctcggaac ctcctcagcc 4861 tgtttgagga caccctagac ccaaccccaa ctttcttgta caaagtgggc ggcaagccca 4921 tccccaaccc cctgctgggc ctggacagca ccgctagcaa agacaatact gtgcctctga 4981 agctgatcgc tctcctggct aatggcgagt tccatagtgg cgaacagctg ggagaaaccc 5041 tgggcatgtc cagggccgct atcaacaagc acattcagac tctgcgcgac tggggcgtgg 5101 acgtgttcac cgtgcccgga aagggctact ctctgcccga gcctatcccg ctgctgaacg 5161 ctaaacagat tctgggacag ctggacggcg ggagcgtggc agtcctgcct gtggtcgact 5221 ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa gagtggggat gcttgcattg 5281 cagaatatca gcaggcaggg agaggaagca gagggaggaa atggttctct ccttttggag 5341 ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg accagcagca atcggcctgg 5401 gcccggtcat cggaattgtc atggcagaag cgctgcgaaa gctgggagca gacaaggtgc 5461 gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa gctggcaggc atcctggtgg 5521 agctggccgg aataacaggc gatgctgcac agatcgtcat tggcgccggg attaacgtgg 5581 ctatgaggcg cgtggaggaa agcgtggtca atcagggctg gatcacactg caggaagcag 5641 ggattaacct ggacaggaat actctggccg ctacgctgat ccgagagctg cgggcagccc 5701 tggaactgtt cgagcaggaa ggcctggctc catatctgcc acggtgggag aagctggata 5761 acttcatcaa tagacccgtg aagctgatca ttggggacaa agagattttc gggattagcc 5821 gggggattga taaacaggga gccctgctgc tggaacagga cggagttatc aaaccctgga 5881 tgggcggaga aatcagtctg cggtctgccg aaaagtaagc caactttatt atacatagtt 5941 gatatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa 6001 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 6061 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 6121 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 6181 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 6241 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 6301 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 6361 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 6421 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 6481 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 6541 tttgggccgc ctccccgcct ggaattctac cgggtagggg aggcgctttt cccaaggcag 6601 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg 6661 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc 6721 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg 6781 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc 6841 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc 6901 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggnnnnnn nggggcgggg 6961 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg 7021 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat 7081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 7141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 7201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 7261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 7321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 7381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 7441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 7501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 7561 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 7621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg 7681 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catctcgagg 7741 gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 7801 aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg 7861 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 7921 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 7981 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 8041 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 8101 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca 8161 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 8221 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc 8281 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 8341 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 8401 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg 8461 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 8521 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 8581 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc 8641 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 8701 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 8761 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 8821 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 8881 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 8941 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 9001 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 9061 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 9121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 9181 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 9241 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 9301 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 9361 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 9421 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 9481 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 9541 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 9601 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 9661 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 9721 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 9781 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 9841 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 9901 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 9961 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 10021 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 10081 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 10141 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 10201 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 10261 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 10321 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 10381 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 10441 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 10501 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 10561 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 10621 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 10681 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 10741 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 10801 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 10861 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 10921 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 10981 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 11041 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 11101 gtgagcgcaa cgcaat //