LOCUS       Exported                8383 bp ds-DNA     circular SYN 07-SEP-2022
DEFINITION  Expresses codon-optimized full length SARS-CoV-2 BA.4.6 Spike.
ACCESSION   .
VERSION     .
KEYWORDS    pCAGGS SARS-CoV-2 BA.4.6 Spike
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8383)
  TITLE     codon-optimized SARS-CoV-2 Spike variants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8383
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gap             30..47
                     /estimated_length=18
     primer_bind     800..822
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     884..903
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(4753..4772)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4818..4873
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4872..4891)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5234..5250)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5234..5250)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5247..5269)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5258..5274
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5282..5312)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5327..5348
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5407..5602
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5453..5588
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5515..5534
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    5608..5742
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5645..5664)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5699..5718
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5810..5827)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5981..6569)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6061..6080)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6740..7600)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7363..7382
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7601..7705)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7736..8115
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggggggggg gggggggggg gggggggggn nnnnnnnnnn nnnnnnnggg gggggggggg
       61 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
      121 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
      181 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
      241 gggggggggg ggggggggng gggggggggg gggggggggg gggggggggg ggngnngggg
      301 gtgtgggcgc gtcggksggg skgsrrscss scstgsrccc ccctccccga gttgctgagc
      361 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg
      421 gcggggggtg gcggcrggkg ggggtgcsgg gcggggsggg gccgccksgg gcncggggrg
      481 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
      541 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
      601 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
      661 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
      721 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
      781 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
      841 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
      901 gtgctgtctc atcattttgg caaagaattc gccaccatgt tcgtcttcct cgtcctcctc
      961 cccctcgtct cctcccagtg cgtcaacctc atcacccgca cccagagcta caccaactcc
     1021 ttcacccgcg gcgtctacta ccccgacaag gtcttccgct cctccgtcct ccactccacc
     1081 caggacctct tcctcccctt cttctccaac gtcacctggt tccacgccat ctccggcacc
     1141 aacggcacca agcgcttcga caaccccgtc ctccccttca acgacggcgt ctacttcgcc
     1201 tccaccgaga agtccaacat catccgcggc tggatcttcg gcaccaccct cgactccaag
     1261 acccagtccc tcctcatcgt caacaacgcc accaacgtcg tcatcaaggt ctgcgagttc
     1321 cagttctgca acgacccctt cctcgacgtc tactaccaca agaacaacaa gtcctggatg
     1381 gagtccgagt tccgcgtcta ctcctccgcc aacaactgca ccttcgagta cgtctcccag
     1441 cccttcctca tggacctcga gggcaagcag ggcaacttca agaacctccg cgagttcgtc
     1501 ttcaagaaca tcgacggcta cttcaagatc tactccaagc acacccccat caacctcggc
     1561 cgcgacctcc cccagggctt ctccgccctc gagcccctcg tcgacctccc catcggcatc
     1621 aacatcaccc gcttccagac cctcctcgcc ctccaccgct cctacctcac ccccggcgac
     1681 tcctcctccg gctggaccgc cggcgccgcc gcctactacg tcggctacct ccagccccgc
     1741 accttcctcc tcaagtacaa cgagaacggc accatcaccg acgccgtcga ctgcgccctc
     1801 gaccccctct ccgagaccaa gtgcaccctc aagtccttca ccgtcgagaa gggcatctac
     1861 cagacctcca acttccgcgt ccagcccacc gagtccatcg tccgcttccc caacatcacc
     1921 aacctctgcc ccttcgacga ggtcttcaac gccaccagct tcgcctccgt ctacgcctgg
     1981 aaccgcaagc gcatctccaa ctgcgtcgcc gactactccg tcctctacaa cttcgcccct
     2041 ttcttcgcct tcaagtgcta cggcgtctcc cccaccaagc tcaacgacct ctgcttcacc
     2101 aacgtctacg ccgactcctt cgtcatccgc ggcaacgagg tcagccagat cgcccccggc
     2161 cagaccggca acatcgccga ctacaactac aagctccccg acgacttcac cggctgcgtc
     2221 atcgcctgga actccaacaa gctcgactcc aaggtcggcg gcaactacaa ctacaggtac
     2281 cgcctcttcc gcaagtccaa cctcaagccc ttcgagcgcg acatctccac cgagatctac
     2341 caggccggca acaaaccctg caacggcgtc gccggcgtga actgctactt ccccctccag
     2401 tcctacggct tcaggcccac ctacggcgtc ggccaccagc cctaccgcgt cgtcgtcctc
     2461 tccttcgagc tcctccacgc ccccgccacc gtctgcggcc ccaagaagtc caccaacctc
     2521 gtcaagaaca agtgcgtcaa cttcaacttc aacggcctca ccggcaccgg cgtcctcacc
     2581 gagtccaaca agaagttcct ccccttccag cagttcggcc gcgacatcgc cgacaccacc
     2641 gacgccgtcc gcgaccccca gaccctcgag atcctcgaca tcaccccctg ctccttcggc
     2701 ggcgtctccg tcatcacccc cggcaccaac acctccaacc aggtcgccgt cctctaccag
     2761 ggtgtcaact gcaccgaggt ccccgtcgcc atccacgccg accagctcac ccccacctgg
     2821 cgcgtctact ccaccggctc caacgtcttc cagacccgcg ccggctgcct catcggcgcc
     2881 gagtacgtca acagctccta cgagtgcgac atccccatcg gcgccggcat ctgcgcctcc
     2941 taccagaccc agaccaagtc ccaccgccgc gcccgctccg tcgcctccca gtccatcatc
     3001 gcctacacca tgtccctcgg cgccgagaac tccgtcgcct actccaacaa ctccatcgcc
     3061 atccccacca acttcaccat ctccgtcacc accgagatcc tccccgtctc catgaccaag
     3121 acctccgtcg actgcaccat gtacatctgc ggcgactcca ccgagtgctc caacctcctc
     3181 ctccagtacg gctccttctg cacccagctc aagcgcgccc tcaccggcat cgccgtcgag
     3241 caggacaaga acacccagga ggtcttcgcc caggtcaagc agatctacaa gacccccccc
     3301 atcaagtact tcggcggctt caacttctcc cagatcctcc ccgacccctc caagccctcc
     3361 aagcgctcct tcatcgagga cctcctcttc aacaaggtca ccctcgccga cgccggcttc
     3421 atcaagcagt acggcgactg cctcggcgac atcgccgccc gcgacctcat ctgcgcccag
     3481 aagttcaacg gcctcaccgt cctccccccc ctcctcaccg acgagatgat cgcccagtac
     3541 acctccgccc tcctcgccgg caccatcacc tccggctgga ccttcggcgc cggcgccgcc
     3601 ctccagatcc ccttcgccat gcagatggcc taccgcttca acggcatcgg cgtcacccag
     3661 aacgtcctct acgagaacca gaagctcatc gccaaccagt tcaactccgc catcggcaag
     3721 atccaggact ccctctcctc caccgcctcc gccctcggca agctccagga cgtcgtcaac
     3781 cacaacgccc aggccctcaa caccctcgtc aagcagctct cctccaagtt cggcgccatc
     3841 tcctccgtcc tcaacgacat cctgtcccgc ctcgacaagg tcgaggccga ggtccagatc
     3901 gaccgcctca tcaccggccg cctccagtcc ctccagacct acgtcaccca gcagctcatc
     3961 cgcgccgccg agatccgcgc ctccgccaac ctcgccgcca ccaagatgtc cgagtgcgtc
     4021 ctcggccagt ccaagcgcgt cgacttctgc ggcaagggct accacctcat gtccttcccc
     4081 cagtccgccc cccacggcgt cgtcttcctc cacgtcacct acgtccccgc ccaggagaag
     4141 aacttcacca ccgcccccgc catctgccac gacggcaagg cccacttccc ccgcgagggc
     4201 gtcttcgtct ccaacggcac ccactggttc gtcacccagc gcaacttcta cgagccccag
     4261 atcatcacca ccgacaacac cttcgtctcc ggcaactgcg acgtcgtcat cggcatcgtc
     4321 aacaacaccg tctacgaccc cctccagccc gagctcgact ccttcaagga ggagctcgac
     4381 aagtacttca agaaccacac ctcccccgac gtcgacctcg gcgacatctc cggcatcaac
     4441 gcctccgtcg tcaacatcca gaaggagatc gaccgcctca acgaggtcgc caagaacctc
     4501 aacgagtccc tcatcgacct ccaggagctc ggcaagtacg agcagtacat caagtggccc
     4561 tggtacatct ggctcggctt catcgccggc ctcatcgcca tcgtcatggt caccatcatg
     4621 ctctgctgca tgacctcctg ctgctcctgc ctcaagggct gctgctcctg cggctcctgc
     4681 tgcaagttcg acgaggacga ctccgagccc gtcctcaagg gcgtcaagct ccactacacc
     4741 taagctagca gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     4801 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     4861 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     4921 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     4981 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     5041 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     5101 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     5161 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
     5221 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     5281 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     5341 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5401 ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     5461 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5521 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     5581 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa
     5641 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5701 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg
     5761 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     5821 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5881 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5941 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6001 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6061 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6121 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6181 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6241 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6301 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6361 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6421 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6481 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6541 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6601 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6661 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6721 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6781 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6841 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6901 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6961 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7021 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7081 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7141 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7201 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7261 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7321 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7381 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7441 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7501 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7561 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7621 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7681 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgacat
     7741 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     7801 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     7861 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     7921 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     7981 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     8041 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     8101 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     8161 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     8221 gcgggggggg ggggggggng cgcnccyngn gggggggggg gggggggggg gggggggggg
     8281 gggggggggg gggggggggg ggggggggng ssnnnggggg ggggggggsg gggggggggg
     8341 gggggggggg gggggggggg gggggggggg gggggggggg ggg
//
