LOCUS Exported 10431 bp ds-DNA circular SYN 21-SEP-2022 DEFINITION MAC-tagged gene expression of human IGF1R. ACCESSION . VERSION . KEYWORDS MAC_C_IGF1R SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10431) TITLE MAC-tagged gene expression REFERENCE 2 (bases 1 to 10431) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 21, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10431 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(155..174) /label=F1ori-R /note="F1 origin, reverse primer" protein_bind 306..353 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS 361..1383 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE " polyA_signal 1513..1634 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1550..1569) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1604..1623 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(1683..1699) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1683..1699) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1696..1718) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1707..1723 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1731..1761) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1776..1797 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(1914..1931) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2085..2673) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2165..2184) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2844..3704) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3467..3486 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3705..3809) /gene="bla" /label=AmpR promoter primer_bind complement(3884..3903) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 4075..4454 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4455..4658 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4609..4629 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 4660..4678 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 4681..4699 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 4709..4733 /label=LNCX /note="Human CMV promoter, forward primer" protein_bind 4876..4900 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(9008..9032) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 9079..9102 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" CDS 9139..9162 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" CDS 9193..9219 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 9220..10179 /codon_start=1 /product="promiscuous R118G mutant of the E. coli biotin protein ligase (Cronan, 2005)" /label=BirA(R118G) /note="also known as BirA* or BioID" /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK" primer_bind complement(10223..10240) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 10229..22 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" ORIGIN 1 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg 61 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 121 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 181 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 241 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 301 acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttccttggcc 361 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc 421 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta 481 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt 541 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg 601 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa 661 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg 721 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc 781 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac 841 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg 901 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc 961 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg 1021 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt 1081 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg 1141 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc 1201 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc 1261 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt 1321 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa 1381 tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga 1441 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc 1501 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 1561 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 1621 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca 1681 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 1741 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 1801 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 1861 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 1921 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 1981 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 2041 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 2101 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 2161 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 2221 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 2281 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 2341 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 2401 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 2461 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 2521 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 2581 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 2641 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 2701 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 2761 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 2821 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 2881 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 2941 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 3001 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 3061 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 3121 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 3181 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 3241 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 3301 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 3361 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 3421 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 3481 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 3541 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 3601 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 3661 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 3721 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 3781 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 3841 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 3901 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 3961 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 4021 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 4081 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 4141 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 4201 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 4261 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 4321 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 4381 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 4441 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 4501 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 4561 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 4621 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 4681 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 4741 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 4801 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga 4861 attctgcaga tatcaacaag tttgtacaaa aaagcaggct ccaccatgaa gtctggctcc 4921 ggaggagggt ccccgacctc gctgtggggg ctcctgtttc tctccgccgc gctctcgctc 4981 tggccgacga gtggagaaat ctgcgggcca ggcatcgaca tccgcaacga ctatcagcag 5041 ctgaagcgcc tggagaactg cacggtgatc gagggctacc tccacatcct gctcatctcc 5101 aaggccgagg actaccgcag ctaccgcttc cccaagctca cggtcattac cgagtacttg 5161 ctgctgttcc gagtggctgg cctcgagagc ctcggagacc tcttccccaa cctcacggtc 5221 atccgcggct ggaaactctt ctacaactac gccctggtca tcttcgagat gaccaatctc 5281 aaggatattg ggctttacaa cctgaggaac attactcggg gggccatcag gattgagaaa 5341 aatgctgacc tctgttacct ctccactgtg gactggtccc tgatcctgga tgcggtgtcc 5401 aataactaca ttgtggggaa taagccccca aaggaatgtg gggacctgtg tccagggacc 5461 atggaggaga agccgatgtg tgagaagacc accatcaaca atgagtacaa ctaccgctgc 5521 tggaccacaa accgctgcca gaaaatgtgc ccaagcacgt gtgggaagcg ggcgtgcacc 5581 gagaacaatg agtgctgcca ccccgagtgc ctgggcagct gcagcgcgcc tgacaacgac 5641 acggcctgtg tagcttgccg ccactactac tatgccggtg tctgtgtgcc tgcctgcccg 5701 cccaacacct acaggtttga gggctggcgc tgtgtggacc gtgacttctg cgccaacatc 5761 ctcagcgccg agagcagcga ctccgagggg tttgtgatcc acgacggcga gtgcatgcag 5821 gagtgcccct cgggcttcat ccgcaacggc agccagagca tgtactgcat cccttgtgaa 5881 ggtccttgcc cgaaggtctg tgaggaagaa aagaaaacaa agaccattga ttctgttact 5941 tctgctcaga tgctccaagg atgcaccatc ttcaagggca atttgctcat taacatccga 6001 cgggggaata acattgcttc agagctggag aacttcatgg ggctcatcga ggtggtgacg 6061 ggctacgtga agatccgcca ttctcatgcc ttggtctcct tgtccttcct aaaaaacctt 6121 cgcctcatcc taggagagga gcagctagaa gggaattact ccttctacgt cctcgacaac 6181 cagaacttgc agcaactgtg ggactgggac caccgcaacc tgaccatcaa agcagggaaa 6241 atgtactttg ctttcaatcc caaattatgt gtttccgaaa tttaccgcat ggaggaagtg 6301 acggggacta aagggcgcca aagcaaaggg gacataaaca ccaggaacaa cggggagaga 6361 gcctcctgtg aaagtgacgt cctgcatttc acctccacca ccacgtcgaa gaatcgcatc 6421 atcataacct ggcaccggta ccggccccct gactacaggg atctcatcag cttcaccgtt 6481 tactacaagg aagcaccctt taagaatgtc acagagtatg atgggcagga tgcctgcggc 6541 tccaacagct ggaacatggt ggacgtggac ctcccgccca acaaggacgt ggagcccggc 6601 atcttactac atgggctgaa gccctggact cagtacgccg tttacgtcaa ggctgtgacc 6661 ctcaccatgg tggagaacga ccatatccgt ggggccaaga gtgagatctt gtacattcgc 6721 accaatgctt cagttccttc cattcccttg gacgttcttt cagcatcgaa ctcctcttct 6781 cagttaatcg tgaagtggaa ccctccctct ctgcccaacg gcaacctgag ttactacatt 6841 gtgcgctggc agcggcagcc tcaggacggc tacctttacc ggcacaatta ctgctccaaa 6901 gacaaaatcc ccatcaggaa gtatgccgac ggcaccatcg acattgagga ggtcacagag 6961 aaccccaaga ctgaggtgtg tggtggggag aaagggcctt gctgcgcctg ccccaaaact 7021 gaagccgaga agcaggccga gaaggaggag gctgaatacc gcaaagtctt tgagaatttc 7081 ctgcacaact ccatcttcgt gcccagacct gaaaggaagc ggagagatgt catgcaagtg 7141 gccaacacca ccatgtccag ccgaagcagg aacaccacgg ccgcagacac ctacaacatc 7201 actgacccgg aagagctgga gacagagtac cctttctttg agagcagagt ggataacaag 7261 gagagaactg tcatttctaa ccttcggcct ttcacattgt accgcatcga tatccacagc 7321 tgcaaccacg aggctgagaa gctgggctgc agcgcctcca acttcgtctt tgcaaggact 7381 atgcccgcag aaggagcaga tgacattcct gggccagtga cctgggagcc aaggcctgaa 7441 aactccatct ttttaaagtg gccggaacct gagaatccca atggattgat tctaatgtat 7501 gaaataaaat acggatcaca agttgaggat cagcgagaat gtgtgtccag acaggaatac 7561 aggaagtatg gaggggccaa gctaaaccgg ctaaacccgg ggaactacac agcccggatt 7621 caggccacat ctctctctgg gaatgggtcg tggacagatc ctgtgttctt ctatgtccag 7681 gccaaaacag gatatgaaaa cttcatccat ctgatcatcg ctctgcccgt cgctgtcctg 7741 ttgatcgtgg gagggttggt gattatgctg tacgtcttcc atagaaagag aaataacagc 7801 aggctgggga atggagtgct gtatgcctct gtgaacccgg agtacttcag cgctgctgat 7861 gtgtacgttc ctgatgagtg ggaggtggct cgggagaaga tcaccatgag ccgggaactt 7921 gggcaggggt cgtttgggat ggtctatgaa ggagttgcca agggtgtggt gaaagatgaa 7981 cctgaaacca gagtggccat taaaacagtg aacgaggccg caagcatgcg tgaaaggatt 8041 gagtttctca acgaagcttc tgtgatgaag gagttcaatt gtcaccatgt ggtgcgattg 8101 ctgggtgtgg tgtcccaagg ccagccaaca ctggtcatca tggaactgat gacacggggc 8161 gatctcaaaa gttatctccg gtctctgagg ccagaaatgg agaataatcc agtcctagca 8221 cctccaagcc tgagcaagat gattcagatg gccggagaga ttgcagacgg catggcatac 8281 ctcaacgcca ataagttcgt ccacagagac cttgctgccc ggaattgcat ggtagccgaa 8341 gatttcacag tcaaaatcgg agattttggt atgacgcgag atatctatga gacagactat 8401 taccggaaag gagggaaagg gctgctgccc gtgcgctgga tgtctcctga gtccctcaag 8461 gatggagtct tcaccactta ctcggacgtc tggtccttcg gggtcgtcct ctgggagatc 8521 gccacactgg ccgagcagcc ctaccagggc ttgtccaacg agcaagtcct tcgcttcgtc 8581 atggagggcg gccttctgga caagccagac aactgtcctg acatgctgtt tgaactgatg 8641 cgcatgtgct ggcagtataa ccccaagatg aggccttcct tcctggagat catcagcagc 8701 atcaaagagg agatggagcc tggcttccgg gaggtctcct tctactacag cgaggagaac 8761 aagctgcccg agccggagga gctggacctg gagccagaga acatggagag cgtccccctg 8821 gacccctcgg cctcctcgtc ctccctgcca ctgcccgaca gacactcagg acacaaggcc 8881 gagaacggcc ccggccctgg ggtgctggtc ctccgcgcca gcttcgacga gagacagcct 8941 tacgcccaca tgaacggggg ccgcaagaac gagcgggcct tgccgctgcc ccagtcttcg 9001 acctgcgacc cagctttctt gtacaaagtg gttgatatca aggccgccga catcaccagc 9061 ctgtacaaga aagtggggtg gagccatccc cagttcgaga agggcggagg atctggcggc 9121 ggaagcggcg gaggaagctg gtcccaccct cagtttgaga aaggcaccga gctgggcagc 9181 accatggcca gctaccccta cgacgtgccc gactacgcca aggacaacac cgtgcccctg 9241 aagctgatcg ccctgctggc caacggcgag ttccactctg gcgagcagct gggcgagaca 9301 ctgggcatga gcagagccgc catcaacaag cacatccaga ccctgcggga ctggggcgtg 9361 gacgtgttca cagtgcccgg caagggctac agcctgcccg agcccatcca gctgctgaac 9421 gccaagcaga tcctgggcca gctggatggc ggatctgtgg ccgtgctgcc cgtgatcgac 9481 agcaccaacc agtatctgct cgaccggatc ggcgagctga agtccggcga cgcctgtatc 9541 gccgagtacc agcaggccgg cagaggcggc aggggcagaa agtggttcag ccccttcggc 9601 gccaacctgt acctgagcat gttctggaga ctggaacagg gcccagccgc cgctattggc 9661 ctgagcctgg tcatcggcat cgtgatggcc gaggtgctgc ggaagctggg cgccgacaaa 9721 gtgcgcgtga agtggcccaa cgacctgtat ctgcaggacc ggaagctggc cggcatcctg 9781 gtggaactga ccggcaaaac aggcgacgcc gcccagatcg tgatcggcgc tggcatcaac 9841 atggccatgc ggcgggtgga agagagcgtg gtcaaccagg gctggatcac cctgcaggaa 9901 gccggcatca acctggaccg gaacaccctg gccgccatgc tgatcagaga gctgagagcc 9961 gccctggaac tgttcgagca ggaaggcctg gccccctacc tgagcagatg ggagaagctg 10021 gacaacttca tcaaccggcc cgtgaaactg atcatcggcg acaaagagat cttcggcatc 10081 agccggggca tcgacaagca gggggccctg ctcctggaac aggacggcat catcaagccc 10141 tggatgggcg gcgagatcag cctgcggagc gccgagaagt gatgactcga gtctagaggg 10201 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 10261 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 10321 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 10381 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc t //