LOCUS       Exported               16577 bp ds-DNA     circular SYN 22-SEP-2022
DEFINITION  Fluorescent reporter for circadian rhythms.
ACCESSION   .
VERSION     .
KEYWORDS    PB-REVERBA-mScarlet-NLS-PEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16577)
  AUTHORS   Zhang ZB, Sinha J, Bahrami-Nejad Z, Teruel MN
  TITLE     The circadian clock mediates daily bursts of cell differentiation by
            periodically restricting cell-differentiation commitment.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Aug 16;119(33):e2204470119. doi: 
            10.1073/pnas.2204470119. Epub 2022 Aug 8.
  PUBMED    35939672
REFERENCE   2  (bases 1 to 16577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 22, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16577
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             4558..5253
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(4691..4709)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4959..4978
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5257..5277
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5544..5665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    5670..5717
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(5832..5851)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5843..6658
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTYGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5916..5935)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     protein_bind    6963..7010
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        10735..11234
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10770..10793)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11167..11186
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             11261..11659
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(11734..11754)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    11796..11917
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   12680..12714
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(13205..13221)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13205..13221)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13218..13240)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13229..13245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(13253..13283)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13298..13319
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13436..13453)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13607..14195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13687..13706)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14366..15226)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14989..15008
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15227..15331)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(15357..15812)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15494..15515)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15706..15725
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15939..15961
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15953..15970
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15954..15970
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   16100..16162
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ttatccgcca tctcagggac gggacggtca caagaaataa gcagtgaatg cctgaccttg
       61 agccagtccc tgagatggcg gtcccttttc ttcttcagta agaggaggga agatcctcac
      121 tccctcctcg cttagtagga gcttgctttg cagctcaagt cagcttcaaa ttcacgacag
      181 tcctcctacc tcagcctctc aaggacggtg attgcaggca cgagccacta cgccctgcca
      241 attcggggag ttgaggggtg ggagttaaat gatcctcatg ttctccctaa actgtgggct
      301 tcttctgcta tcatgaagcc ctgcagagag cagacccccc tctctctatg aggacctcag
      361 agcactttcc ctttgttctc ctttttattt cccagacaaa gggaaatgac tcaccccaaa
      421 gtcacttgag tgggcagtgt ggtgtcatat aagcaaacag ggagtcccta gggatatccc
      481 cgtgctatgc cagtcctacc cgtgggggct ggggttaggg tgaagaagag aaaggggttt
      541 tatttacact gcttgtcgcc caccttgtca ccatagcaaa agctgcttaa gttcttgatt
      601 taagggaaat ctctcctccc agcttgtctt tctcccccta gtcaccacta acctcagggt
      661 gaggtcagtt ctccaatcac gtgaagctct cacgtttgct aggtttgcag aaagggcctt
      721 ttagctttga tcttccacgg agcagtaaag cttgccaggc cctccccagg aattcacatg
      781 cccttgccat acaaggcttt ccaaacacgc caccctgact cttcagaaaa ccccacccca
      841 gtccactttc agctcctccc cgctgccagc aagcactttt gcaaagcaga aaaggaatag
      901 gcacactcca cctacattgt cagtagacta caaatcccaa caatcctggc ggtgcgcagg
      961 cgcacagatc tcaacgtgcc ggctgctgga aaagtgtgtc actggggcac gaggcgctcc
     1021 ctggaatcac atggtacctg ctccagtgcc gcgtgcggcc cgggaaccct gggctgctgg
     1081 cgcctgcgca gagccctctg tcccagggaa aggctcgggc aaaaggcggt tgagattggc
     1141 agagtgaaat attactgctg aggaaacgta gcagggcaca cgtctctctt tgcgtcctgg
     1201 tgcctcattt ctccatcacc tacttacttc ctggttgcag cttctcttcc tttgggactt
     1261 ttgcacccgg agctccagat tcattaccct gcttcactgc ggagccttca ggagtggcat
     1321 ccggtgcact gcagagaccc gactctcctt gctaccttat agccagaact gctgcaggct
     1381 gattcttcac acacacactc tctgctcttc ccatgcaaat cagatctcag gtgcctcagc
     1441 gtcctaccct tctggagggc tgcagtatag ccaccccaag accttactgc tcagtgcctg
     1501 gaatcctgat tgcgaactgc ggggttcact cgtctccctc agccattgcc cagggggcga
     1561 gagaggccat cacaacctcc agtttgtgtc aaggtccagt ttgaatgacc gctttcagct
     1621 ggtgaagaca tgacgaccct ggactccaat aacaacacag gtactgagat tcttatcttt
     1681 gctctgtcac tgtctccatt tccatccaag gcttatgaga ggaactttgt aagggattgg
     1741 ggattccctc tggctggtgt ttgttactgt gctaagatga aagccactag gtaagatttc
     1801 taaagagggt tattacggct gggaatgtgc ctaaaacgca tcactagcac cccctgagtt
     1861 cccctcagtc cgagaacgga tgaaatggag ggtggatacc agtggcgagt ggaagagttt
     1921 agggagactc agcattttga agcagtctct ggacacgtcc ccatttgccc actccttgca
     1981 gctatagtat gcctgtctct tttccctttt ctacagttct agcctacttc caggtagtct
     2041 tgggaagtgg ggcgatccgg gctggtgggt ttgcgtgact gggtcagagt tcacagggga
     2101 atcctcagcg gcagtgggga gcttcatttg aggagtcggg tgcttgggac tcatctccag
     2161 gcagggcacc cacagcgact tagcctgggg agggctcaca tggctgcaga gccgggccca
     2221 cgtgctgcat ttgttttcat tcagtagaag ccgtggccgc catctcattg gtcattgcct
     2281 cccggcctcg ttacataatg agctccgccc ctcaagttgc cggagcaacg cttgtcgcct
     2341 tctctcctcc cctcagttgt cccggcccag cttggtttta gggccagcct acgacccagg
     2401 acgcccggga ggggcggggc gagggcgggg ttttccttgc gacagaaggg aagcggagaa
     2461 ggcggtgctt cgagttccgg ggttcctcgt tggggtgcca cgtgcgaggg gcacacgtgg
     2521 agtggggacg tgaggccagc tgagggccag gctcccgccc cgcccttctc cgcctagatc
     2581 tgggtgaagc tgggctgact caccgccccc gcctggcttc ttttcctctc agactgcctg
     2641 gcccttaccc tctatgaccg agacttgagt aatatggggg cacccctgac ttgtttggcc
     2701 tcaagtttat acgtcccagc taaacctcac accagattat tcttataccc ctttattcta
     2761 atcatgccta cattcatttt ctgcattcag actcactttt ccatgttcca aagtttactg
     2821 ttctccgagt atgactttgc atgccatgaa tgtcttctcc cccccaaaca ggaatacctt
     2881 agaatcaagg ttgacaacgt actactttgt gtcgtcgccc agggtttttt ccccccactc
     2941 atagaaagga aaactgagac caagagacaa gagttgattt cctctatatt ggaaagctaa
     3001 tcggtagcaa tacacatcta gatggagagc tagggtcttt tgagcattgc ccatcacttc
     3061 aaagttggaa gacttttttt tttagtgact ttctcatgcc ctctttcagg attaactagg
     3121 tcaagttcat ttaggtccac ataaataacc catcctccaa cccagccgtg aaaccatagc
     3181 cgggtaacca ttctgtgact tattttacag gcagggttca ccctgaccaa ccttgagcta
     3241 ttttaaggtt gccctgcctg gtttagtgtg tgtcagtggg tgagcaagac atgtgcttct
     3301 gtcgactcgg tggcatggca tgtgtcacct cacacacgtg tctgaggatc ctcccctttc
     3361 cccactccac ccatggccgg ccaggctaag tacttggcag aggtgtggca gacaagtggc
     3421 ccttctcttc tgtgcatacc acaggagccg aatcacctgg cccgcaatgt aaccagaggg
     3481 acagagccgc tggctgagtg gctatagagc taaagttacc accgggctga gaaggaaggg
     3541 gaattcctaa atccctagaa tggatacttt gtttctccct ttcatctcct gcaactcatc
     3601 tgagtcttat ctgtcacatt tctaagtctc ttgtgactca tctctcagag gccagagcca
     3661 ggctgaacag agagtccttc cctgcagtac tctatgagaa tatgtctttg ggtttgaaag
     3721 caaggtgtga ccgttttacg tttgtacacc tccattttcc tcgcactagg ctagctaccc
     3781 atcacgctgc agggtccatc actggaaagg tctcagtgta gaactagtgg ctagtttttt
     3841 ttgtgtgtgt gtgtatgaat aactgtcagc tgtccatctc cttcccaagt ctacccccct
     3901 tgtaactaag accctagctg ctgtcagtac tccccaggaa gcagctagct caggggttcc
     3961 catgtatgag atggccactg gagttacacc tcccacccaa accagaagct taatgtattg
     4021 tgcaaagcaa tggccatggg tattttgggg ccggacttgg ttatcataag tagcatgtga
     4081 tggagagttt ctaataacga ctaggttgac cggccgtgct atatttagtc tgtgtgggag
     4141 aattgcttct tactccaagg ttagggggtc taatgttctg atgatgactg tgtgaatgac
     4201 agacttgatg gctgataccc tccccttgtg ttctcttatt taggtggtgt tatcacctac
     4261 attggctcta gtggctcctc cccgagccgg accagcccgg agtccctcta cagtgacagc
     4321 tccaatggca gcttccagtc cctgactcaa ggttgtccca catacttccc accatcacct
     4381 actggctccc tcacccagga ccctgcccgc tcttttggca gtgcgccacc cagtctcagt
     4441 gatgatagct ccccttcttc tacatcatcg tcatcctctt catcctcctc ctccttctat
     4501 aacgggagcc ccccaggaag tctacaagtg gccatggaag acagcagccg agtgtccatg
     4561 gtgagcaagg gcgaggcagt gatcaaggag ttcatgcggt tcaaggtgca catggagggc
     4621 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     4681 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttctc ctgggacatc
     4741 ctgtcccctc agttcatgta cggctccagg gccttcatca agcaccccgc cgacatcccc
     4801 gactactata agcagtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     4861 gacggcggcg ccgtgaccgt gacccaggac acctccctgg aggacggcac cctgatctac
     4921 aaggtgaagc tccgcggcac caacttccct cctgacggcc ccgtaatgca gaagaagaca
     4981 atgggctggg aagcgtccac cgagcggttg taccccgagg acggcgtgct gaagggcgac
     5041 attaagatgg ccctgcgcct gaaggacggc ggccgctacc tggcggactt caagaccacc
     5101 tacaaggcca agaagcccgt gcagatgccc ggcgcctaca acgtcgaccg caagttggac
     5161 atcacctccc acaacgagga ctacaccgtg gtggaacagt acgaacgctc cgagggccgc
     5221 cactccaccg gcggcatgga cgagctgtac aagcctccaa aaaagaagag aaaggtagaa
     5281 gaccccttgt acaagagcca tggcttcccg ccggctgtgg ctgctcagga tgatggcacg
     5341 ctgcccatgt cctgtgccca ggagagcggg atggaccgtc accctgcagc ctgtgctcct
     5401 gctaggatca atgtgtagat gcgcggccgc gactctagat cataatcagc cataccacat
     5461 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     5521 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     5581 gcaatagcat cacaaatttc acaaataaag catttttttc gctgcattct agttgtggtt
     5641 tgtccaaact catcaatgta tcttaaaacg aagttcctat tccgaagttc ctattctcta
     5701 gaaagtatag gaacttcgtt atcggatcct agtaagccac gttgtgtctc aaaatctctg
     5761 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     5821 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc
     5881 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     5941 cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt
     6001 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     6061 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     6121 tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata
     6181 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     6241 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     6301 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     6361 gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt
     6421 cgtcacttat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     6481 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     6541 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     6601 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     6661 agaattggtt aattggttgt agcactggca gagcattacg ctgacttgac gggacggcgg
     6721 ctttgttgaa taaatcgaac tcttgctgag ttgaaggatc agatcacgca tcttcccgac
     6781 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac
     6841 aaagctctca tcaaccgtgg ctccctcact ttccggctgg atgatggggc gattcaggcc
     6901 tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcggcggcc ggatccgata
     6961 acgaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc gttttaaggc
     7021 gtaaattgta agcgttaata atcccaggtc acccgggccc atcgagaaat cttcacctat
     7081 gcccatgaca agttaggcac ctcacctggt aacttcaatg ccaatcatgc atcaggtagc
     7141 ccttcagcta caactccaca ccgctgggag agtcagggat gcccgtctgc ccccaacgac
     7201 aacaaccttt tggcggctca gcgtcataat gaagcgctga atggtctacg ccagggccct
     7261 tcctcctacc cgcctacctg gccttctggc cccacccacc acagctgcca ccaacctaac
     7321 agcaatgggc accgtctgtg ccccacccac gtatattcgg ccccagaagg ggaggcacct
     7381 gccaacagtc tacggcaagg caacaccaag aatgttctgc tggtgagggc atggagccag
     7441 tgtggagaga gcgagggggc cttggaagat tgggactaga gaaagacagc cagtgttcct
     7501 agaggctggg ggtggggact tctgtccagg aaaggacatt ctaccccaaa atctgggttt
     7561 tacctgggaa tccttaatca ccattcggca cctgattacc gatttaggtg agagacccct
     7621 tctcaagggc cacagctctt agcccagaca gtacttggaa ccagtcctgt tggggagccc
     7681 acccagctaa catgtccctc cttactccat tctcctctta ggcatgtccc atgaacatgt
     7741 atccccatgg acgcagcggc cggactgtgc aggagatctg ggaagacttc tctatgagct
     7801 tcacgcctgc cgttcgggag gtggtagagt ttgccaaaca catcccaggc ttccgtgacc
     7861 tttctcagca cgaccaggtg accctgctta aggctggcac ctttgaggtg agcggtactt
     7921 ttatgttccc tgctcttgac tgtgggaggc ccccagtagt gtggtgactg gagtgggaag
     7981 ggcttcccag agtctttggc cctgtgttat ctctctgtct gaggagatag aacttgtaac
     8041 accgctagct cggcacctca gggtctgtgg gaagtctatc ctctaggctg gtgttgcctc
     8101 caaactcagt gcatcagagt aaccaaagag acaactctcc tctactctag gccggagctg
     8161 gtgaccttcg gttaaattac attccagaag tggcgggcac tcccacctgc atccctcatt
     8221 gaccaatcag agactgaccc ccttactgac aatctaagga aacacccctc aaagttatta
     8281 gcaggctcta ggggtcattt ggcaaaatgc ccctcccatc tcactcaaaa aatgtccacc
     8341 ctcccttcag gtgctgatgg tgcgctttgc atcgttgttc aacgtgaagg accagacagt
     8401 gatgttcctg agccgcacaa cctacagtct gcaggagctc ggtgccatgg gcatgggcga
     8461 cctgctcaat gccatgtttg acttcagcga gaagctcaac tccctggcac ttaccgagga
     8521 ggagctgggc ctattcaccg cggtggtgct tgtctctgca ggtgagccaa gctctcccct
     8581 gaccctggag gaggcacacg cagttcaatt caacatacct tttatggaga acctactcta
     8641 tgccaggccc agcactgggc actgggctac agacatgatt cagacacagt ctagcttggg
     8701 aaacagactc atgcccgact aattataaaa cagcgagaag tgtcacacaa gaagcatgaa
     8761 ccaagtaagc acagacgact atttctgccc gggagagctg gggaaggagg tgacacttga
     8821 tgtggggcct caaagggaca agataactgc caggcataga gagggggaag ggcatcccag
     8881 gcagagggaa ctacagagca aataacaagg gagcttggcc acgagtggca tgctcagggt
     8941 aggagggtgg gctcgaggag tgggaggtgg ccagcctgtg gccatgctcc cagcgtgtgg
     9001 gcgtggccct gctctagggc agaggccccc cagggagaag gagtagcgcc ctccttctcc
     9061 tttcccgttg ccctccgttg ccctccgagg ggcctcaggg agaggctgcc ttgcctgcct
     9121 ggtcctcgca gacctccgtg tcctccgtgt cagaggagga gctcagggag ctcgcctcac
     9181 tgctgtcgtc ttccccacag accgctcggg aatggagaat tccgcttcgg tggagcagct
     9241 ccaggagacg ctgctgcggg ctcttcgggc tctggtgctg aagaaccggc cctcggagac
     9301 ttcccgcttc accaagctgc tgctcaagct gccggacctg cggaccctga acaacatgca
     9361 ttccgagaag ctgctgtcct tccgggtgga cgcccagtga cccgcccggc cggccttctg
     9421 ccgctgcccc cttgtacaga atcgaactct gcacttctct ctcctttacg agacgaaaag
     9481 gaaaagcaaa ccagaatctt atttatattg ttataaaata ttccaagatg agcctctggc
     9541 cccctgagcc ttcttgtaaa taactcttcc cccaccccca ccgccatgct cccatcctcc
     9601 cctatttaaa ccactcttgc tctctccacc ctcctctggc ccctcgattt gttctgttcc
     9661 tgtctcaaat ccaatagttc acagctgagc tggcttctgt gatgtgtgct cgtttgggga
     9721 attggggtgg gggtggggga ctactgccaa gggggtgtgt ggggggggga agacacctct
     9781 ccatctggtc agcttgtggg gtgggtactc tgccagcaga tgggagaata tggggaaaca
     9841 gcacgcaagt ctaagggggg gtgcccaagg gggttatttt catcatatat ttattacata
     9901 aatatatagt aaaatagacg agcaacaatt accataaaaa tatctttctt taaaatcctg
     9961 acccaaaaca caacggggtg aaaaattcgc acataacaga catactgatt gtcaactgtg
    10021 gggcaggagg aggagcccct caagtcaccc ctgccggtcc cacccatctc tggggaaagg
    10081 acctatctcc ttccctgttt ctagccacaa cccctccccc cacctcagca ccagcttctc
    10141 atacattcag tagcgcctct gggtagtgtc cccctcctcc acccaggagt tcctagggca
    10201 ggaagtaatt tcccccttat ggatggacag acggtgtcca tgtcacacgc cctgctctct
    10261 cccagccctg tagacccacc gcccggggaa ggggtagggg ctcctcctcc cccaccctcc
    10321 ccaagtctga gcagggagga ggctggatga ggtatgtgtg ttataagtga tggggggggg
    10381 aatactgaga tggagaggca gtggtagggc agggtagtgg tcatggctct ggcaccccct
    10441 tgtcctggcc tggccccccc aaccttctcc cagcaaccca cccctcccat caatactgca
    10501 cctgctgccc cacccggcct gccctagtgc cccgggccaa cattggggga aggcatgcta
    10561 agcacccatg ccaaggccat ccccccatta gcaccaatgg cccccaggag agggccctgt
    10621 cccccacttc catggcaggg actgggacag ctgcctctct agagcaggtg cgtggcactc
    10681 ctgagtctct ctggctaagg ccgcacgcgt atttaaattc tagagaattc taccgggtag
    10741 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
    10801 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
    10861 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
    10921 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
    10981 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
    11041 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
    11101 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
    11161 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
    11221 ctccgggcct ttcgacctgc agcccaagct tcctaggacc atggccaagc ctttgtctca
    11281 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
    11341 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
    11401 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
    11461 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
    11521 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
    11581 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
    11641 ttatgtgtgg gagggctaac ccgggcgggg cgcgtctggc aatcaacctc tggattacaa
    11701 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
    11761 cgctgcttta atgcctttgt atcatgcgtt aactaaactt gtttattgca gcttataatg
    11821 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    11881 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggaat tgactcaaat
    11941 gatgtcaatt agtctatcag aagctcatct ggtctccctt ccgggggaca agacatccct
    12001 gtttaatatt taaacagcag tgttcccaaa ctgggttctt atatcccttg ctctggtcaa
    12061 ccaggttgca gggtttcctg tcctcacagg aacgaagtcc ctaaagaaac agtggcagcc
    12121 aggtttagcc ccggaattga ctggattcct tttttagggc ccattggtat ggctttttcc
    12181 ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg
    12241 atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg
    12301 gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga
    12361 gggacgtaat tacatccctg ggggctttgg gggggggctg tccctgatat ctataacaag
    12421 aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg
    12481 tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg
    12541 tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc
    12601 caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga
    12661 gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaatc
    12721 tagctgcatc aggatcatat cgtcgggtct tttttccggc tcagtcatcg cccaagctgg
    12781 cgctatctgg gcatcgggga ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca
    12841 tggaaagagt ttgccgagga tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt
    12901 accatgatga ttcgggaagg tgtggccatg cacgccttta acggtgaact gttcgttcag
    12961 gccacctggg ataccagttc gtcgcggctt ttccggacac agttccggat ggtcagcccg
    13021 aagcgcatca gcaacccgaa caataccggc gacagccgga actgccgtgc cggtgtgcag
    13081 attaatgaca gcggtgcggc gctgggatat tacgtcagcg aggacgggta tcctggctgg
    13141 atgccgcaga aatggacatg gataccccgt gagttacccg gcgggcgcgc ttggcgtaat
    13201 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
    13261 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    13321 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    13381 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
    13441 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    13501 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    13561 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    13621 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    13681 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    13741 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    13801 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    13861 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    13921 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    13981 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    14041 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    14101 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    14161 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    14221 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    14281 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    14341 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    14401 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    14461 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    14521 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    14581 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    14641 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    14701 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    14761 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    14821 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    14881 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    14941 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    15001 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    15061 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    15121 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    15181 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    15241 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    15301 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
    15361 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
    15421 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
    15481 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
    15541 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
    15601 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
    15661 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
    15721 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
    15781 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
    15841 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    15901 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    15961 gacggccagt gagcgcgcct cgttcattca cgtttttgaa cccgtggagg acgggcagac
    16021 tcgcggtgca aatgtgtttt acagcgtgat ggagcagatg aagatgctcg acacgctgca
    16081 gaacacgcag ctagattaac cctagaaaga taatcatatt gtgacgtacg ttaaagataa
    16141 tcatgtgtaa aattgacgca tgtgttttat cggtctgtat atcgaggttt atttattaat
    16201 ttgaatagat attaagtttt attatattta cacttacata ctaataataa attcaacaaa
    16261 caatttattt atgtttattt atttattaaa aaaaacaaaa actcaaaatt tcttctataa
    16321 agtaacaaaa cttttatgag ggacagcccc cccccaaagc ccccagggat gtaattacgt
    16381 ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt ccggcgctcc
    16441 ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg
    16501 gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tggggggata
    16561 cggggaaaag gcctcca
//
