LOCUS Exported 4180 bp ds-DNA circular SYN 23-SEP-2022 DEFINITION 3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259). ACCESSION . VERSION . KEYWORDS pRSV-Rev SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4180) AUTHORS Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L TITLE A third-generation lentivirus vector with a conditional packaging system. JOURNAL J Virol. 1998 Nov . 72(11):8463-71. PUBMED 9765382 REFERENCE 2 (bases 1 to 4180) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 23, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4180 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 147..166 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 400..417 /label=L4440 /note="L4440 vector, forward primer" protein_bind 534..555 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 570..600 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 608..624 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 613..635 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 632..648 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 632..648 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 793..1054 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" primer_bind 1065..1089 /label=LNCX /note="Human CMV promoter, forward primer" CDS 1183..1533 /codon_start=1 /gene="rev" /product="human immunodeficiency virus 1 (HIV-1) Rev protein" /label=Rev /note="enhances nuclear export of unspliced and incompletely spliced viral mRNAs" /translation="MAGRSGDSDEDLLKAVRLIKFLYQSNPPPNPEGTRQARRNRRRRW RERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILV ESPTILESGAKE" primer_bind complement(1617..1634) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(1617..1633) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 1965..2420 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2052..2071) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2262..2283 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2437..2456) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 2556..2578 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(2616..2634) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 2702..2806 /gene="bla" /label=AmpR promoter CDS 2807..3667 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3025..3044) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3838..246 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 61 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 121 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 181 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 241 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 301 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 361 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 421 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 481 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 541 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 601 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat 661 tcgatgtacg ggccagatat acgcgtatct gaggggacta gggtgtgttt aggcgaaaag 721 cggggcttcg gttgtacgcg gttaggagtc ccctcaggat atagtagttt cgcttttgca 781 tagggagggg gaaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat 841 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt 901 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg 961 aaccactgaa ttccgcattg cagagatatt gtatttaagt gcctagctcg atacaataaa 1021 cgccatttga ccattcacca cattggtgtg cacctccaag ctcgagctcg tttagtgaac 1081 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac 1141 cgatccagcc tcccctcgaa gctagtcgat taggcatctc ctatggcagg aagaagcgga 1201 gacagcgacg aagacctcct caaggcagtc agactcatca agtttctcta tcaaagcaac 1261 ccacctccca atcccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 1321 gagagacaga gacagatcca ttcgattagt gaacggatcc ttagcactta tctgggacga 1381 tctgcggagc ctgtgcctct tcagctacca ccgcttgaga gacttactct tgattgtaac 1441 gaggattgtg gaacttctgg gacgcagggg gtgggaagcc ctcaaatatt ggtggaatct 1501 cctacaatat tggagtcagg agctaaagaa tagtgctgtt agcttgctca atgccacagc 1561 tatagcagta gctgagggga cagatagggt tatagaagta gtacaagaag cttggcactg 1621 gccgtcgttt tacaacgtcg tgatctgagc ctgggagatc tctggctaac tagggaaccc 1681 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 1741 gtgtgactct ggtaactaga gatcaggaaa accctggcgt tacccaactt aatcgccttg 1801 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 1861 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc 1921 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg 1981 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 2041 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 2101 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 2161 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac 2221 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 2281 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat 2341 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 2401 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata 2461 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct 2521 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 2581 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata 2641 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 2701 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 2761 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 2821 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 2881 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 2941 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 3001 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 3061 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 3121 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 3181 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 3241 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 3301 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 3361 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 3421 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 3481 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 3541 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 3601 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 3661 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 3721 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 3781 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 3841 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 3901 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 3961 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 4021 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 4081 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 4141 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc //