LOCUS       Exported                 900 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Human OCT4 genomic targeting donor.
ACCESSION   .
VERSION     .
KEYWORDS    OCT4-2A-eGFP-PGK-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Hockemeyer D, Wang H, Kiani S, Lai CS, Gao Q, Cassady JP, Cost GJ, 
            Zhang L, Santiago Y, Miller JC, Zeitler B, Cherone JM, Meng X, 
            Hinkley SJ, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Genetic engineering of human pluripotent cells using TALE nucleases.
  JOURNAL   Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927.
  PUBMED    21738127
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(21..77)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(864..880)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(864..880)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(877..899)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 ctctcgccct tgctcaccat tggcccggga ttctcttcga catcccctgc ttgtttcaac
       61 agggagaagt tagtggcgct agcgtttgaa tgcatgggag agcccagagt ggtgacggag
      121 acagggggaa aggcttcccc ctcagggaaa gggaccgagg agtacagtgc agtgaagtga
      181 gggctcccat agcctggggt accaaaatgg ggccctgggg ccagaggaaa ggacactggt
      241 ccccctgaga aaggagaccc agcagcctca aaatcctctc gttgtgcata gtcgctgctt
      301 gatcgcttgc ccttctggcg ccggttacag aaccacactc ggaccacctg caagtgaatg
      361 acagaaagga gaatgacatt agacaatgag ctgagacggg cctgactctg cttggacatt
      421 ctatccaaag ccaacagccc tagagcagtt agaggaggac attagagaat gagctgagac
      481 aggcctgact gcttggacat tctgtccaaa gccaacagcc ctagagcagt tggaggagcc
      541 agagctaggg aaagcgaggt ggtgacaggg gaaagagatg gagcccgcag agagacatgg
      601 cactcacatc cttctcgagc ccaagctgct gggcgatgtg gctgatctgc tgcagtgtgg
      661 gtttcgggca ctgcaggaac aaattctcca ggttgcctct cactcggttc tcgatactgg
      721 ttcgctttct ctttcgggcc tgcacgaggg tttctgcttt gcatatctgt gcaggtggga
      781 cctgcaggaa aagggcgaat tccagcacac tggcggccgt tactagtgga tccgagctcg
      841 gtaccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
//
