LOCUS       Exported                8424 bp ds-DNA     circular SYN 19-OCT-2022
DEFINITION  Polycistronic human KLF4, MYC and SOX2 were cloned into the pMXs 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    pMXs-KMS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Wang L, Huang D, Huang C, Yin Y, Vali K, Zhang M, Tang Y
  TITLE     Enhanced human somatic cell reprogramming efficiency by fusion of 
            the MYC transactivation domain and OCT4.
  JOURNAL   Stem Cell Res. 2017 Dec;25:88-97. doi: 10.1016/j.scr.2017.10.014. 
            Epub 2017 Oct 26.
  PUBMED    29125994
REFERENCE   2  (bases 1 to 8424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 19, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             3343..3399
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3400..4716
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     CDS             4726..4785
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     LTR             5929..6398
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6528..6545)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6699..7287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6779..6798)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7451..8311)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8074..8093
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8312..8416)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccatggctg tcagcgacgc gctgctccca tctttctcca cgttcgcgtc tggcccggcg
     1981 ggaagggaga agacactgcg tcaagcaggt gccccgaata accgctggcg ggaggagctc
     2041 tcccacatga agcgacttcc cccagtgctt cccggccgcc cctatgacct ggcggcggcg
     2101 accgtggcca cagacctgga gagcggcgga gccggtgcgg cttgcggcgg tagcaacctg
     2161 gcgcccctac ctcggagaga gaccgaggag ttcaacgatc tcctggacct ggactttatt
     2221 ctctccaatt cgctgaccca tcctccggag tcagtggccg ccaccgtgtc ctcgtcagcg
     2281 tcagcctcct cttcgtcgtc gccgtcgagc agcggccctg ccagcgcgcc ctccacctgc
     2341 agcttcacct atccgatccg ggccgggaac gacccgggcg tggcgccggg cggcacgggc
     2401 ggaggcctcc tctatggcag ggagtccgct ccccctccga cggctccctt caacctggcg
     2461 gacatcaacg acgtgagccc ctcgggcggc ttcgtggccg agctcctgcg gccagaattg
     2521 gacccggtgt acattccgcc gcagcagccg cagccgccag gtggcgggct gatgggcaag
     2581 ttcgtgctga aggcgtcgct gagcgcccct ggcagcgagt acggcagccc gtcggtcatc
     2641 agcgtcagca aaggcagccc tgacggcagc cacccggtgg tggtggcgcc ctacaacggc
     2701 gggccgccgc gcacgtgccc caagatcaag caggaggcgg tctcttcgtg cacccacttg
     2761 ggcgctggac cccctctcag caatggccac cggccggctg cacacgactt ccccctgggg
     2821 cggcagctcc ccagcaggac taccccgacc ctgggtcttg aggaagtgct gagcagcagg
     2881 gactgtcacc ctgccctgcc gcttcctccc ggcttccatc cccacccggg gcccaattac
     2941 ccatccttcc tgcccgatca gatgcagccg caagtcccgc cgctccatta ccaagagctc
     3001 atgccacccg gttcctgcat gccagaggag cccaagccaa agaggggaag acgatcgtgg
     3061 ccccggaaaa ggaccgccac ccacacttgt gattacgcgg gctgcggcaa aacctacaca
     3121 aagagttccc atctcaaggc acacctgcga acccacacag gtgagaaacc ttaccactgt
     3181 gactgggacg gctgtggatg gaaattcgcc cgctcagatg aactgaccag gcactaccgt
     3241 aaacacacgg ggcaccgccc gttccagtgc caaaaatgcg accgagcatt ttccaggtcg
     3301 gaccacctcg ccttacacat gaagaggcat tttggatctg gagcaacaaa cttctcacta
     3361 ctcaaacaag caggtgacgt ggaggagaat cccggcccta tgcccctcaa cgttagcttc
     3421 accaacagga actatgacct cgactacgac tcggtgcagc cgtatttcta ctgcgacgag
     3481 gaggagaact tctaccagca gcagcagcag agcgagctgc agcccccggc gcccagcgag
     3541 gatatctgga agaaattcga gctgctgccc accccgcccc tgtcccctag ccgccgctcc
     3601 gggctctgct cgccctccta cgttgcggtc acacccttct cccttcgggg agacaacgac
     3661 ggcggtggcg ggagcttctc cacggccgac cagctggaga tggtgaccga gctgctggga
     3721 ggagacatgg tgaaccagag tttcatctgc gacccggacg acgagacctt catcaaaaac
     3781 atcatcatcc aggactgtat gtggagcggc ttctcggccg ccgccaagct cgtctcagag
     3841 aagctggcct cctaccaggc tgcgcgcaaa gacagcggca gcccgaaccc cgcccgcggc
     3901 cacagcgtct gctccacctc cagcttgtac ctgcaggatc tgagcgccgc cgcctcagag
     3961 tgcatcgacc cctcggtggt cttcccctac cctctcaacg acagcagctc gcccaagtcc
     4021 tgcgcctcgc aagactccag cgccttctct ccgtcctcgg attctctgct ctcctcgacg
     4081 gagtcctccc cgcagggcag ccccgagccc ctggtgctcc atgaggagac accgcccacc
     4141 accagcagcg actctgagga ggaacaagaa gatgaggaag aaatcgatgt tgtttctgtg
     4201 gaaaagaggc aggctcctgg caaaaggtca gagtctggat caccttctgc tggaggccac
     4261 agcaaacctc ctcacagccc actggtcctc aagaggtgcc acgtctccac acatcagcac
     4321 aactacgcag cgcctccctc cactcggaag gactatcctg ctgccaagag ggtcaagttg
     4381 gacagtgtca gagtcctgag acagatcagc aacaaccgaa aatgcaccag ccccaggtcc
     4441 tcggacaccg aggagaatgt caagaggcga acacacaacg tcttggagcg ccagaggagg
     4501 aacgagctaa aacggagctt ttttgccctg cgtgaccaga tcccggagtt ggaaaacaat
     4561 gaaaaggccc ccaaggtagt tatccttaaa aaagccacag catacatcct gtccgtccaa
     4621 gcagaggagc aaaagctcat ttctgaagag gacttgttgc ggaaacgacg agaacagttg
     4681 aaacacaaac ttgaacagct acggaactct tgtgcgggat ctggacaatg tactaactac
     4741 gctttgttga aactcgctgg cgatgttgaa agtaaccccg gtcccatgta caacatgatg
     4801 gagacggagc tgaagccgcc gggcccgcag caaacttcgg ggggcggcgg cggcaactcc
     4861 accgcggcgg cggccggcgg caaccagaaa aacagcccgg accgcgtcaa gcggcccatg
     4921 aatgccttca tggtgtggtc ccgcgggcag cggcgcaaga tggcccagga gaaccccaag
     4981 atgcacaact cggagatcag caagcgcctg ggcgccgagt ggaaactttt gtcggagacg
     5041 gagaagcggc cgttcatcga cgaggctaag cggctgcgag cgctgcacat gaaggagcac
     5101 ccggattata aataccggcc ccggcggaaa accaagacgc tcatgaagaa ggataagtac
     5161 acgctgcccg gcgggctgct ggcccccggc ggcaatagca tggcgagcgg ggtcggggtg
     5221 ggcgccggcc tgggcgcggg cgtgaaccag cgcatggaca gttacgcgca catgaacggc
     5281 tggagcaacg gcagctacag catgatgcag gaccagctgg gctacccgca gcacccgggc
     5341 ctcaatgcgc acggcgcagc gcagatgcag cccatgcacc gctacgacgt gagcgccctg
     5401 cagtacaact ccatgaccag ctcgcagacc tacatgaacg gctcgcccac ctacagcatg
     5461 tcctactcgc agcagggcac ccctggcatg gctcttggct ccatgggttc ggtggtcaag
     5521 tccgaggcca gctccagccc ccctgtggtt acctcttcct cccactccag ggcgccctgc
     5581 caggccgggg acctccggga catgatcagc atgtatctcc ccggcgccga ggtgccggaa
     5641 cccgccgccc ccagcagact tcacatgtcc cagcactacc agagcggccc ggtgcccggc
     5701 acggccatta acggcacact gcccctctca cacatgtgag gcggccgcca gcacagtggt
     5761 cgacgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca
     5821 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat
     5881 aactgagaat agagaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc
     5941 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa
     6001 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
     6061 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga
     6121 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
     6181 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
     6241 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc
     6301 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
     6361 ctctgagtga ttgactaccc gtcagcgggg gtctttcaca tgcagcatgt atcaaaatta
     6421 atttggtttt ttttcttaag tatttacatt aaatggccat agttgcatta atgaatcggc
     6481 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6541 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6601 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6661 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6721 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6781 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6841 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6901 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6961 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7021 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7081 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7141 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7201 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7261 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7321 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7381 ttcacctaga tccttttgcg gccggccgca aatcaatcta aagtatatat gagtaaactt
     7441 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7501 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7561 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7621 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7681 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7741 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7801 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7861 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7921 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7981 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8041 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8101 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8161 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8221 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8281 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8341 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8401 aaataggggt tccgcgcaca tttc
//
