LOCUS Exported 2746 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pHD_v2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2746) AUTHORS Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F TITLE A transcription activator-like effector toolbox for genome engineering. JOURNAL Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. PUBMED 22222791 REFERENCE 2 (bases 1 to 2746) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2746 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(810..901) /gene="bla" /label=AmpR promoter primer_bind 968..987 /label=pENTR-R /note="pENTR vectors, reverse primer" primer_bind complement(1748..1765) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(1748..1764) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1863..1880) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2034..2622) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2114..2133) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2746..809) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" ORIGIN 1 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata 61 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat 121 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct 181 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact 241 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag 301 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc 361 gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgag 421 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat 481 tcttctaata cctggaacgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca 541 tcaggagtac ggataaaatg cttgatggtc ggaagtggca taaattccgt cagccagttt 601 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac 661 aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca 721 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc 781 ctcgacgttt cccgttgaat atggctcata ttcttccttt ttcaatatta ttgaagcatt 841 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 901 ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata 961 cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc 1021 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc 1081 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact 1141 gggcctttcg cccgggctaa ttagggggtg tcgcccttcg ctgaactcac cccagagcag 1201 gtcgtggcaa ttgcgagcca tgacggggga aagcaggcac tcgaaaccgt ccagaggttg 1261 ctgcctgtgc tgtgccaagc gcacggacgt caaaagggcg acacaaaatt tattctaaat 1321 gcataataaa tactgataac atcttatagt ttgtattata ttttgtatta tcgttgacat 1381 gtataatttt gatatcaaaa actgattttc cctttattat tttcgagatt tattttctta 1441 attctcttta acaaactaga aatattgtat atacaaaaaa tcataaataa tagatgaata 1501 gtttaattat aggtgttcat caatcgaaaa agcaacgtat cttatttaaa gtgcgttgct 1561 tttttctcat ttataaggtt aaataattct catatatcaa gcaaagtgac aggcgccctt 1621 aaatattctg acaaatgctc tttccctaaa ctccccccat aaaaaaaccc gccgaagcgg 1681 gtttttacgt tatttgcgga ttaacgatta ctcgttatca gaaccgccca gggggcccga 1741 gcttaagact ggccgtcgtt ttacaacaca gaaagagttt gtagaaacgc aaaaaggcca 1801 tccgtcaggg gccttctgct tagtttgatg cctggcagtt ccctactctc gccttccgct 1861 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 1921 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 1981 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 2041 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 2101 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 2161 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 2221 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 2281 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 2341 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 2401 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg ggctaactac 2461 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 2521 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 2581 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 2641 tctacggggt ctgacgctca gtggaacgac gcgcgcgtaa ctcacgttaa gggattttgg 2701 tcatgagctt gcgccgtccc gtcaagtcag cgtaatgctc tgcttt //