LOCUS       Exported               12217 bp ds-DNA     circular SYN 06-JAN-2023
DEFINITION  Second-generation mammalian acoustic reporter gene derived from 
            Anabaena.
ACCESSION   .
VERSION     .
KEYWORDS    mARGAna (cassette 2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12217)
  AUTHORS   Robert C. Hurt, Marjorie T. Buss, Mengtong Duan, Katie Wong, Mei Yi 
            You, Daniel P. Sawyer, Margaret B. Swift, Przemysław Dutka, Pierina 
            Barturen-Larrea, David R. Mittelstein, Zhiyang Jin, Mohamad H. 
  TITLE     Genomically mined acoustic reporter genes for real-time in vivo 
            monitoring of tumors and tumor-homing bacteria
  JOURNAL   Nature Biotechnology, 2 January 2023
REFERENCE   2  (bases 1 to 12217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 6, 2023 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12217
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(171..1031)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1905..1967
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2401..2750
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    2409..2427
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2445..2463
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2481..2499
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2517..2535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2553..2571
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2589..2607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2625..2643
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2689..2713
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2764..2773
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3796..3852
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4615..4671
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5155..5211
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5965..6021
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6397..6453
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7150..7206
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7567..7623
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(7930..7949)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(8431..8450)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8432..8450)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    8845..9433
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8898..8918)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9316..9327)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    9453..9929
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(9652..9671)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   10702..10736
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(11227..11243)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11227..11243)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11240..11262)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11251..11267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11275..11305)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11320..11341
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11458..11475)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11629..12217)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11709..11728)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
     1801 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
     1861 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
     1921 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc
     1981 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
     2041 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
     2101 caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc
     2161 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
     2221 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
     2281 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
     2341 gagcctgcag acacctgggg ggatacgggg aaaaggcctc cacggccact agtttcactc
     2401 gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag
     2461 agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc
     2521 tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta
     2581 cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
     2641 agaacgtatg tcgaggtagg cgtgtacggt gggcgcctat aaaagcagag ctcgtttagt
     2701 gaaccgtcag atcgcctgga gcaattccac aacacttttg tcttatactt ggtacctatg
     2761 tccgccacca tggtgaccac caccaaagtg aaccacaagc gggccgtgct gagactgagg
     2821 cctggccagt ttgtcgtgac ccccgccatt gagcgggtgg ccattagagc cctgagatac
     2881 ctgaagtccg gcttccccgt gcacctgaga ggacctgccg gaaccggcaa gaccacactg
     2941 gccatgcacc tggccaactg cctggacaga cccgtgatgc tgctgttcgg cgacgaccag
     3001 ttcaagagca gcgacctgat cggcagcgag agcggctaca cccacaagaa ggtgctggac
     3061 aactacatcc acagcgtcgt gaagctggaa gatgagttca agcagaactg ggtggacagc
     3121 agactgaccc tggcctgccg ggaaggcttc accctggtgt acgacgagtt caaccggtcc
     3181 agacccgaag tgaacaacgt gctgctgagc gccctggaag agaagatcct gagcctgccc
     3241 cccagcagca accagccaga gtacctgagc gtgaaccccc agttcagagt gatcttcacc
     3301 agcaaccccg aggaatatgc cggcgtgcac agcacccagg acgccctgat ggaccggctc
     3361 gtgaccatct ccatgcccga gcccgatgag atcacccaga ccgagatcct gatccagaaa
     3421 acaaacatcg accgcgagag cgccaacttc atcgtgcggc tcgtgaagtc cttcagactg
     3481 gccacaggcg ccgagaaaac cagcggcctg agaagctgcc tgatgatcgc caaagtgtgc
     3541 gccgacaaca acatccccgt gaccaccgag agcctggact tccccgatat cgccatcgac
     3601 atcctgttca accgcagcca cctgagcatg agcgagtcca ccaacatctt tctggaactg
     3661 ctggataagt tcagcgccga ggaactggaa atcctgaaca acagagtgac cggcgacaac
     3721 gacttcctga tcgacaacag ccagttcgtg tcccagcagc tggccggaca gcccaacgga
     3781 gcgccaggtt ccggggctac taacttcagc ctccttaaac aggctggcga tgtggaagaa
     3841 aatcccggac cagtgctgcc caccagaccc cagaccaaca gcagccggac catcaacacc
     3901 agcacccagg gcagcaccct ggccgacatc ctggaaagag tgctggacaa gggcatcgtg
     3961 atcgccgggg acatcagcat ctctatcgcc agcaccgagc tggtgcacat ccggatcaga
     4021 ctgctgatct ccagcgtgga caaggccaaa gagatgggca tcaactggtg ggagagcgac
     4081 ccctacctga gcaccaaggc ccagagactg gtggaagaga accagcagct ccagcacaga
     4141 ctggaaagcc tggaagccaa gctgaacagc ctgaccagca gcagcgtgaa agaggaaatc
     4201 cccctggccg ccgacgtgaa ggacgacctg tatcagacca gcgccaagat ccccagcccc
     4261 gtggataccc ctatcgaggt gctggacttc caggcccagt ctagcggcgg cacaccccct
     4321 tacgtgaaca cctctatgga aatactggat tttcaggctc agacctccgc cgagagcagc
     4381 agccctgtgg gctctaccgt ggaaatactg gacttccagg cacagacaag cgaggaatcc
     4441 agctcccccg tggtgtccac agtggaaata ctggatttcc aggcccagac ttctgaagag
     4501 tccagctctc cagtgggaag cactgtggaa atactggact ttcaggccca gaccagtgaa
     4561 gagatcccca gctctgtgga ccccgccatc gatgtgggtg ccccgggatc tggcgcaaca
     4621 aattttagtc tcctcaagca ggcaggagat gtcgaggaaa accctggacc cgtggtgtgc
     4681 acccctgccg agaacttcaa caacagcctg acaatcgcca gcaagcccaa gaacgaggcc
     4741 ggactggctc ctctgctgct gaccgtgctg gaactcgtgc ggcagctgat ggaagcccaa
     4801 gtgatccggc ggatggaaga ggacctgctg agcgagcccg acctggaaag agccgccgat
     4861 agcctccaga agctggaaga acagatcctg cacctgtgcg agatgttcga ggtggacccc
     4921 gccgacctga acatcaacct gggcgagatc ggcaccctgc tgcctagcag cggcagctac
     4981 tatcctggcc agccaagcag cagaccctct gtgctggaac tgctggaccg gctgctgaac
     5041 accggcatcg tggtggacgg cgagattgac ctgggaatcg cccagatcga cctgatccac
     5101 gccaagctga gactggtgct gacctccaag cccatcgggg cacctggctc aggagcgacc
     5161 aacttctcat tgctcaaaca agccggagat gttgaggaga atccaggccc tgtgagcatc
     5221 cccctgtacc tgtacggcat cttccccaac accatccccg agacactgga actggaaggc
     5281 ctggacaagc agcccgtgca cagccaggtg gtggacgagt tctgcttcct gtacagcgag
     5341 gcccggcagg aaaagtacct ggccagcaga cggaacctgc tgacccacga gaaggtgctg
     5401 gaacagacca tgcacgccgg cttcagagtg ctgctgcccc tgagatttgg cctggtcgtg
     5461 aaggactggg agacaatcat gagccagctg atcaaccccc acaaggacca gctgaaccag
     5521 ctgttccaga agctggccgg caagcgggaa gtgtccatca agattttctg ggacgccaag
     5581 gccgagcttc agaccatgat ggaaagccac caggacctga agcagcagcg ggacaacatg
     5641 gaaggcaaga aactgagcat ggaagaagtg atccagatcg ggcagctgat cgagatcaac
     5701 ctgctggccc ggaagcaggc cgtgatcgag gtgttcagcc aggaactgaa ccccttcgcc
     5761 caggaaatcg tggtgtccga ccccatgacc gaggaaatga tctacaacgc cgccttcctg
     5821 atcccctggg agagcgagag cgagttcagc gagcgggtgg aagtgatcga ccagaagttc
     5881 ggcgaccggc tgcggatcag atacaacaac ttcaccgccc cctacacctt tgcccagctg
     5941 gactctggcg ctcccgggtc aggtgccact aatttttcat tgttgaagca agctggggat
     6001 gttgaagaga acccagggcc tgtgctgacc aaactcctgc tgctgcccat catgggaccc
     6061 ctgaatggcg tcgtgtggat cgccgagcag atccaggaac ggaccaacac cgagttcgac
     6121 gcccaggaaa acctgcacaa acagctgctg agcctccagc tgagcttcga catcggcgag
     6181 atcggagagg aagagttcga gattcaggaa gaggaaatcc tgctgaagat ccaggccctg
     6241 gaagaagagg cccggctgga actggaagcc gagcaggaag aagccagact ggaactggaa
     6301 gctgaacagg aagatttcga gtacccacca cagttcaccg ccgaagtgaa caaggatcag
     6361 catctggtgc tgctgcccgg tgccccggga tctggcgcta ctaacttcag cctccttaaa
     6421 caggctggcg atgtggaaga aaatcccgga ccagtggaac tggaaaacct gtacacctac
     6481 gcctttctgg aaatccccag cagccccctg atcctgcctc agggcgctgc taatcaggtg
     6541 gtgctgatca acggcaccga gctggccgcc atcgtggaac ctggcatctt cctggaatcc
     6601 ttccagaaca acgacgagaa gatcatccag atggccctga gccacgacag agtgatctgc
     6661 gagctgttcc agcagatcac cgtgctgccc ctgagattcg gcacctactt caccagcacc
     6721 aacaacctgc tgaaccacct gaagtcccac gagaaagagt accagaacaa gctggaaaag
     6781 atcaacggga agaacgagtt caccctgaag ctgatccccc ggatgatcga ggaaatcgtg
     6841 ccctctgagg gcggaggcaa ggactacttc ctggccaaga agcagcgcta ccagaatcag
     6901 aacaacttct ctatcgccca ggccgccgag aagcagaacc tgatcgacct gatcaccaaa
     6961 gtgaaccagc tgcccgtggt ggtgcaggaa caggaagaac agatccaaat ctacctgctg
     7021 gtgtcctgcc aggataagac cctgctgctg gaacagttcc tgacctggca gaaagcctgc
     7081 cccaagtggg atctgctgct gggcgactgc ctgcccccct accactttat cggagcgcca
     7141 ggttccgggg caacaaattt tagtctcctc aagcaggcag gagatgtcga ggaaaaccct
     7201 ggacccgtga tcaagaacat ccaggtgttc tttatgaaaa ccatcagcaa ccggtccatc
     7261 agccgggcca agatcagcac catgcccaga cctaagagcg acgccagcag ccagctggac
     7321 ctgtacaaga tggtcaccga gaagcagaga atccagcggg atatgtacag catcaaagaa
     7381 cggatgggcc tgctccagca gcggctggac atcctgaacc agcagatcga ggccaccgag
     7441 aaaaccatcc acaagctgcg gcagccccac agcaacaccg cccagaatat cgtgcggagc
     7501 aacatcttcg tggaaagcaa caactaccag accttcgagg tggaatacgg ggcacctggc
     7561 tcaggagcca ctaatttttc attgttgaag caagctgggg atgttgaaga gaacccaggg
     7621 cctgtggtgt ccaagggcga ggaactgttc accggcgtgg tgcccatcct ggtggaactg
     7681 gatggcgacg tgaacggcca caagttcagc gtgtccggcg agggcgaagg cgacgccaca
     7741 tacggaaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccttggcct
     7801 accctcgtga ccacactgac ctacggcgtg cagtgcttcg ccagataccc cgaccacatg
     7861 aagcagcacg atttcttcaa gagcgccatg cccgagggct acgtgcagga acggaccatc
     7921 ttcttcaagg acgacggcaa ctacaagaca agagccgaag tgaagttcga gggcgacacc
     7981 ctcgtgaacc ggatcgagct gaagggcatc gacttcaaag aggatggcaa catcctgggc
     8041 cacaagctgg agtacaacta caacagccac aaggtgtaca tcaccgccga caagcagaaa
     8101 aacggcatca aagtgaactt caagacccgg cacaacatcg aggacggcag cgtgcagctg
     8161 gccgaccact accagcagaa cacccccatc ggagatggcc ccgtgctgct gcccgacaac
     8221 cactacctga gcacacaaag cgccctgagc aaggacccca acgagaagcg ggaccacatg
     8281 gtgctgctgg aatttgtgac cgccgctggc atcaccctgg gcatggacga gctgtacaag
     8341 tgactcgagt ctagagggcc ccgtggctgt aatctagagg atccctcgag gggcccaagc
     8401 ttacgcgtgc atgcgacgtc atagctctct ccctatagtg agtcgtatta taagctagct
     8461 tgggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     8521 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactagc
     8581 tgcatatgct tgctgcttga gagttttgct tactgagtat gatttatgaa aatattatac
     8641 acaggagcta gtgattctaa ttgtttgtgt attttagatt cacagtccca aggctcattt
     8701 caggcccctc agtcctcaca gtctgttcat gatcataatc agccatacca catttgtaga
     8761 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     8821 tgcaattgtt gttgttcgtg gctgaatcaa cctctggatt acaaaatttg tgaaagattg
     8881 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     8941 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     9001 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     9061 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     9121 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     9181 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     9241 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     9301 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     9361 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     9421 gccgcctccc cgccagagac aattaacttc gcgggtggca tccctgtgac ccctccccag
     9481 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
     9541 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
     9601 tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg
     9661 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
     9721 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
     9781 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
     9841 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
     9901 cgtgaaccac tgctcccttc cctgtccttg aagttctcat catgtctgga attgactcaa
     9961 atgatgtcaa ttagtctatc agaagctcat ctggtctccc ttccggggga caagacatcc
    10021 ctgtttaata tttaaacagc agtgttccca aactgggttc ttatatccct tgctctggtc
    10081 aaccaggttg cagggtttcc tgtcctcaca ggaacgaagt ccctaaagaa acagtggcag
    10141 ccaggtttag ccccggaatt gactggattc cttttttagg gcccattggt atggcttttt
    10201 ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag
    10261 cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg
    10321 cggggggagc gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg
    10381 gagggacgta attacatccc tgggggcttt gggggggggc tgtccctgat atctataaca
    10441 agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat
    10501 cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc
    10561 ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc
    10621 tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga
    10681 gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa
    10741 tctagctgca tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct
    10801 ggcgctatct gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg
    10861 catggaaaga gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt
    10921 ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc
    10981 aggccacctg ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc
    11041 cgaagcgcat cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc
    11101 agattaatga cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct
    11161 ggatgccgca gaaatggaca tggatacccc gtgagttacc cggcgggcgc gcttggcgta
    11221 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    11281 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    11341 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    11401 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    11461 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    11521 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    11581 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    11641 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    11701 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    11761 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    11821 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    11881 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    11941 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    12001 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    12061 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    12121 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    12181 caagcagcag attacgcgca gaaaaaaagg atctcaa
//
