LOCUS       Exported                9973 bp ds-DNA     circular SYN 03-NOV-2022
DEFINITION  AAVS1 donor vector for genomic targeting.
ACCESSION   .
VERSION     .
KEYWORDS    AAVS1-Neo-M2rtTA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9973)
  AUTHORS   DeKelver RC, Choi VM, Moehle EA, Paschon DE, Hockemeyer D, Meijsing 
            SH, Sancak Y, Cui X, Steine EJ, Miller JC, Tam P, Bartsevich VV, 
            Meng X, Rupniewski I, Gopalan SM, Sun HC, Pitz KJ, Rock JM, Zhang L,
            Davis GD, Rebar EJ, Cheeseman IM, Yamamoto KR, Sabatini DM, Jaenisch
            R, Gregory PD, Urnov FD
  TITLE     Functional genomics, proteomics, and regulatory DNA analysis in 
            isogenic settings using zinc finger nuclease-driven transgenesis 
            into a safe harbor locus in the human genome.
  JOURNAL   Genome Res. 2010 Aug;20(8):1133-42. doi: 10.1101/gr.106773.110. Epub
            2010 May 27.
  PUBMED    20508142
REFERENCE   2  (bases 1 to 9973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 3, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9973
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     intron          110..1126
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1050..1072
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1134..1153
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1226..1972
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    1982..2116
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2006..2025)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2060..2079
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    2456..3292
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(3326..3345)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3327..3345)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3352..3369)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3352..3368)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3361..3383)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             3506..3808
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3687..3706
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(5205..6065)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5828..5847
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6189..6777
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6678..6697
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6931..6948
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7065..7086
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7101..7131
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7139..7155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7144..7166
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7163..7179
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7163..7179
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7200..7218
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    7253..8056
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    8063..8088
                     /label=SA
                     /note="splice acceptor site"
     CDS             8112..8165
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8181..8984
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    8994..9455
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     enhancer        9553..9856
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gsggrgaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
       61 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
      121 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
      181 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
      241 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
      301 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
      361 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
      421 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc
      481 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
      541 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg
      601 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct
      661 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
      721 ggccggggrg ggctcggggg rggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
      781 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
      841 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
      901 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
      961 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     1021 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     1081 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     1141 gctggttatt gtgctgtctc atcattttgg caaagaattc ggcttgatcg aagctagtgg
     1201 atcccccggg ctgcaggaat tcaccatgtc tagactggac aagagcaaag tcataaacgg
     1261 cgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga aactcgctca
     1321 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
     1381 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
     1441 cgagtcatgg caagactttc tgcggaacaa cgccaagtca ttccgctgtg ctctcctctc
     1501 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
     1561 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
     1621 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
     1681 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
     1741 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
     1801 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccgg ccgacgccct
     1861 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat
     1921 gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa
     1981 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     2041 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     2101 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     2161 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     2221 tcctgcaagc ctcgtcgtcc tggccggacc acgctatctg tgcaaggtcc ccggccccgg
     2281 acgcgcgctc catgagcaga gcgcccgccg ccgaggcgaa gactcgggcg gcgccctgcc
     2341 cgtcccacca ggtcaacagg cggtaaccgg cctcttcatc gggaatgcgc gcgaccttca
     2401 gcatcgccgg catgtccccc tggcggacgg gaagtatcca gctcgaccaa gctttactag
     2461 ggacaggatt ggtgacagaa aagccccatc cttaggcctc ctccttccta gtctcctgat
     2521 attgggtcta acccccacct cctgttaggc agattcctta tctggtgaca cacccccatt
     2581 tcctggagcc atctctctcc ttgccagaac ctctaaggtt tgcttacgat ggagccagag
     2641 aggatcctgg gagggagagc ttggcagggg gtgggaggga agggggggat gcgtgacctg
     2701 cccggttctc agtggccacc ctgcgctacc ctctcccaga acctgagctg ctctgacgcg
     2761 gctgtctggt gcgtttcact gatcctggtg ctgcagcttc cttacacttc ccaagaggag
     2821 aagcagtttg gaaaaacaaa atcagaataa gttggtcctg agttctaact ttggctcttc
     2881 acctttctag tccccaattt atattgttcc tccgtgcgtc agttttacct gtgagataag
     2941 gccagtagcc agccccgtcc tggcagggct gtggtgagga ggggggtgtc cgtgtggaaa
     3001 actccctttg tgagaatggt gcgtcctagg tgttcaccag gtcgtggccg cctctactcc
     3061 ctttctcttt ctccatcctt ctttccttaa agagtcccca gtgctatctg ggacatattc
     3121 ctccgcccag agcagggtcc cgcttcccta aggccctgct ctgggcttct gggtttgagt
     3181 ccttggcaag cccaggagag gcgctcaggc ttccctgtcc cccttcctcg tccaccatct
     3241 catgcccctg gctctcctgc cccttcccta caggggttcc tggctctgct ctaagggcga
     3301 attcgcggcc gctaaattca attcgcccta tagtgagtcg tattacaatt cactggccgt
     3361 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     3421 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     3481 acagttgcgc agcctatacg tacggcagtt taaggtttac acctataaaa gagagagccg
     3541 ttatcgtctg tttgtggatg tacagagtga tattattgac acgccggggc gacggatggt
     3601 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     3661 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     3721 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     3781 cattaacctg atgttctggg gaatataaat gtcaggcatg agattatcaa aaaggatctt
     3841 cacctagatc cttttcacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa
     3901 tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc
     3961 ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc
     4021 ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat
     4081 ggctttcttg ccgccaagga tctgatggcg caggggatca agctctgatc aagagacagg
     4141 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4201 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4261 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4321 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4381 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4441 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4501 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4561 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4621 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4681 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat gcatggcgat gcctgcttgc
     4741 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     4801 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     4861 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     4921 tcgccttcta tcgccttctt gacgagttct tctgaattat taacgcttac aatttcctga
     4981 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatcagg tggcactttt
     5041 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5101 ccgctcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     5161 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5221 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5281 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5341 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5401 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5461 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5521 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5581 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5641 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5701 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgaatac
     5761 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5821 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5881 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5941 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     6001 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     6061 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgacc
     6121 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6181 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6241 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6301 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6361 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6421 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6481 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6541 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6601 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6661 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6721 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6781 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6841 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     6901 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     6961 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     7021 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     7081 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     7141 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctcaga
     7201 attaaccctc actaaaggga ctagtcctgc aggtttaaac gaattcgccc tttgctttct
     7261 ctgaccagca ttctctcccc tgggcctgtg ccgctttctg tctgcagctt gtggcctggg
     7321 tcacctctac ggctggccca gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc
     7381 actccctctt ccccttgctc tctgctgtgt tgctgcccaa ggatgctctt tccggagcac
     7441 ttccttctcg gcgctgcacc acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc
     7501 ctctccgggc atctctcctc cctcacccaa ccccatgccg tcttcactcg ctgggttccc
     7561 ttttccttct ccttctgggg cctgtgccat ctctcgtttc ttaggatggc cttctccgac
     7621 ggatgtctcc cttgcgtccc gcctcccctt cttgtaggcc tgcatcatca ccgtttttct
     7681 ggacaacccc aaagtacccc gtctccctgg ctttagccac ctctccatcc tcttgctttc
     7741 tttgcctgga caccccgttc tcctgtggat tcgggtcacc tctcactcct ttcatttggg
     7801 cagctcccct acccccctta cctctctagt ctgtgctagc tcttccagcc ccctgtcatg
     7861 gcatcttcca ggggtccgag agctcagcta gtcttcttcc tccaacccgg gcccctatgt
     7921 ccacttcagg acagcatgtt tgctgcctcc agggatcctg tgtccccgag ctgggaccac
     7981 cttatattcc cagggccggt taatgtggct ctggttctgg gtacttttat ctgtcccctc
     8041 caccccacag tggggcaagc ttctgacctc ttctcttcct cccacagggc ctcgagagat
     8101 ctggcagcgg agagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg
     8161 gccctaggct cgagacgcgt atgggatcgg ccattgaaca agatggattg cacgcaggtt
     8221 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     8281 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     8341 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     8401 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     8461 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     8521 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     8581 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     8641 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     8701 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     8761 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     8821 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     8881 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     8941 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaggggat ccgctgtaag
     9001 tctgcagaaa ttgatgatct attaaacaat aaagatgtcc actaaaatgg aagtttttcc
     9061 tgtcatactt tgttaagaag ggtgagaaca gagtacctac attttgaatg gaaggattgg
     9121 agctacgggg gtgggggtgg ggtgggatta gataaatgcc tgctctttac tgaaggctct
     9181 ttactattgc tttatgataa tgtttcatag ttggatatca taatttaaac aagcaaaacc
     9241 aaattaaggg ccagctcatt cctcccactc atgatctata gatctataga tctctcgtgg
     9301 gatcattgtt tttctcttga ttcccacttt gtggttctaa gtactgtggt ttccaaatgt
     9361 gtcagtttca tagcctgaag aacgagatca gcagcctctg ttccacatac acttcattct
     9421 cagtattgtt ttgccaagtt ctaattccat cagaagctgg gagctccacc gcggtggcgg
     9481 ccgctctaga actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     9541 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     9601 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     9661 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     9721 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     9781 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     9841 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
     9901 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
     9961 ggcggggggg ggg
//
