LOCUS Exported 866 bp ds-DNA linear SYN 10-AUG-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS TAL024 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 866) AUTHORS Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR TITLE Targeted gene disruption in somatic zebrafish cells using engineered TALENs. JOURNAL Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. PUBMED 21822241 REFERENCE 2 (bases 1 to 866) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 10, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..866 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 14..36 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 28..45 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 29..45 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(256..272) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(256..272) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(269..291) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 280..296 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(304..334) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 349..370 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(487..504) /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(738..757) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt 61 acctcgcgaa tgcatctaga gaagacaatt gacgcctgca caagtggtcg ccatcgccaa 121 caacaacggc ggtaagcagg cgctggaaac agtacagcgc ctgctgcctg tactgtgcca 181 ggatcatgga ctgaagagac cggatcccgg gcccgtcgac tgcagaggcc tgcatgcaag 241 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 301 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 361 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 421 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 481 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 541 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 601 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 661 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 721 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 781 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 841 ggcgctttct catagctcac gctgta //