LOCUS Exported 878 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 878) AUTHORS Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, Scharenberg AM TITLE Tracking genome engineering outcome at individual DNA breakpoints. JOURNAL Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648. PUBMED 21743461 REFERENCE 2 (bases 1 to 878) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..878 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 8..34 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 44..64 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 785..838 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" ORIGIN 1 gggcgtatac ccctacgacg tgcccgacta cgcccccggg ccccctaaga aaaagaggaa 61 ggtgaagaac atcaagaaga accaggtcat gaacctgggc cccaacagca agctgctgaa 121 ggagtacaag agccagctga tcgagctgaa catcgagcag ttcgaggccg gcatcggcct 181 gatcctgggc gacgcctaca tcaggagcag ggacgagggc aagacctact gcatgcagtt 241 cgagtggaag aacaaggcct acatggacca cgtgtgcctg ctgtacgacc agtgggtgct 301 gagccccccc cacaagaagg agagggtgaa ccacctgggc aacctggtca tcacctgggg 361 cgcccagacc ttcaagcacc aggccttcaa caagctggcc aacctgttca tcgtgaacaa 421 caagaagacc atccccaaca acctggtgga gaactacctg acccccatga gcctggccta 481 ctggttcatg gacgacggcg gcaagtggga ctacaacaag aacagcacca acaagagcat 541 cgtgctgaac acccagagct tcaccttcga ggaggtggag tacctggtga agggcctgag 601 gaacaagttc cagctgaact gctacgtgaa gatcaacaag aacaagccca tcatctacat 661 cgacagcatg agctacctga tcttctacaa cctgatcaag ccctacctga tcccccagat 721 gatgtacaag ctgcccaaca ccatcagcag cgagaccttc ctgaagggcg gcggcggatc 781 cggtgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc cgggccccat 841 ggctcgggac cctctgccat tctttccacc tctgtacc //