LOCUS Exported 825 bp ds-DNA linear SYN 10-JUL-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS TAL034 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 825) AUTHORS Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR TITLE Targeted gene disruption in somatic zebrafish cells using engineered TALENs. JOURNAL Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. PUBMED 21822241 REFERENCE 2 (bases 1 to 825) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 10, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..825 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 19..41 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 33..50 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 34..50 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(261..277) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(261..277) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(274..296) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 285..301 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(309..339) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 354..375 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(492..509) /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(743..762) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc 61 tcggtacctc gcgaatgcat ctagagaaga caactgactc ccgatcaagt tgtagcgatt 121 gcgaataaca atggagggaa acaagcattg gagactgtcc aacggctcct tcccgtgttg 181 tgtcaagccc acggtctgaa gagaccggat cccgggcccg tcgactgcag aggcctgcat 241 gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 301 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 361 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 421 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 481 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 541 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 601 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 661 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 721 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 781 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcc //