LOCUS       Exported                 897 bp ds-DNA     linear   SYN 07-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    JDS74
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR
  TITLE     Targeted gene disruption in somatic zebrafish cells using engineered
            TALENs.
  JOURNAL   Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934.
  PUBMED    21822241
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             80..145
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             152..172
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcaccatgg actacaaaga ccatgacggt gattataaag atcatgacat
      121 cgattacaag gatgacgatg acaagatggc ccccaagaag aagaggaagg tgggcattca
      181 ccgcggggta cctatggtgg acttgaggac actcggttat tcgcaacagc aacaggagaa
      241 aatcaagcct aaggtcagga gcaccgtcgc gcaacaccac gaggcgcttg tggggcatgg
      301 cttcactcat gcgcatattg tcgcgctttc acagcaccct gcggcgcttg ggacggtggc
      361 tgtcaaatac caagatatga ttgcggccct gcccgaagcc acgcacgagg caattgtagg
      421 ggtcggtaaa cagtggtcgg gagcgcgagc acttgaggcg ctgctgactg tggcgggtga
      481 gcttaggggg cctccgctcc agctcgacac cgggcagctg ctgaagatcg cgaagagagg
      541 gggagtaaca gcggtagagg cagtgcacgc ctggcgcaat gcgctcaccg gggccccctt
      601 gaacagagac gattaatgcg tctcgctgac acccgaacag gtggtcgcca ttgctaataa
      661 taacggagga cggccagcct tggagtccat cgtagcccaa ttgtccaggc ccgatcccgc
      721 gttggctgcg ttaacgaatg accatctggt ggcgttggca tgtcttggtg gacgacccgc
      781 gctcgatgca gtcaaaaagg gtctgcctca tgctcccgca ttgatcaaaa gaaccaaccg
      841 gcggattccc gagagaactt cccatcgagt cgcgggatcc caactagtca aaagtga
//
