LOCUS       Exported               13444 bp ds-DNA     circular SYN 13-DEC-2022
DEFINITION  episomal vector expressing mCherry + human SOX2A61V + KLF4.
ACCESSION   .
VERSION     .
KEYWORDS    pCXLE-mCherry-t2a-SOX2AV-p2a-KLF4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   MacCarthy CM, Malik V, Wu G, Velychko T, Keshet G, Jauch R, Cojocaru
            V, Scholer HR, Velychko S
  TITLE     Enhancing Sox/Oct cooperativity induces higher-grade developmental 
            reset
  JOURNAL   bioRxiv 2022.09.23.509242
REFERENCE   2  (bases 1 to 13444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 13, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13444
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..662
                     /label=chicken beta-actin promoter
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1744..2451
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1889..1907)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2157..2176
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2467..2520
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3481..3537
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    5022..5610
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5075..5095)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5493..5504)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5701..5720)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5766..5821
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5820..5839)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6182..6198)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6182..6198)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6195..6217)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6206..6222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6230..6260)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6275..6296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    complement(6385..6418)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             6879..8804
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    complement(8972..9005)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      9192..10981
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(11522..11539)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11693..12281)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11773..11792)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12452..13312)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13075..13094
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13313..13417)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcgcccttc
     1741 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     1801 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     1861 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     1921 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     1981 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     2041 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     2101 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     2161 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     2221 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     2281 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     2341 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     2401 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtataa ggcatgcggc
     2461 tccggcgagg gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggccca
     2521 atgtacaaca tgatggagac ggagctgaag ccgccgggcc cgcagcaaac ttcggggggc
     2581 ggcggcggca actccaccgc ggcggcggcc ggcggcaacc agaaaaacag cccggaccgc
     2641 gtcaagcggc ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg caagatggcc
     2701 caggagaacc ccaagatgca caactcggag atcagcaagc gcctgggcgc cgagtggaaa
     2761 cttttgtcgg agacggagaa gcggccgttc atcgacgagg ctaagcggct gcgagtgctg
     2821 cacatgaagg agcacccgga ttataaatac cggccccggc ggaaaaccaa gacgctcatg
     2881 aagaaggata agtacacgct gcccggcggg ctgctggccc ccggcggcaa tagcatggcg
     2941 agcggggtcg gggtgggcgc cggcctgggc gcgggcgtga accagcgcat ggacagttac
     3001 gcgcacatga acggctggag caacggcagc tacagcatga tgcaggacca gctgggctac
     3061 ccgcagcacc cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat gcaccgctac
     3121 gacgtgagcg ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg
     3181 cccacctaca gcatgtccta ctcgcagcag ggcacccctg gcatggctct tggctccatg
     3241 ggttcggtgg tcaagtccga ggccagctcc agcccccctg tggttacctc ttcctcccac
     3301 tccagggcgc cctgccaggc cggggacctc cgggacatga tcagcatgta tctccccggc
     3361 gccgaggtgc cggaacccgc cgcccccagc agacttcaca tgtcccagca ctaccagagc
     3421 ggcccggtgc ccggcacggc cattaacggc acactgcccc tctcacacat gggcaccggt
     3481 gccaccaact tcagcctgct gaagcaggcc ggcgacgtgg aggagaaccc cggccccatg
     3541 aacaattcgc ccttcaccat ggctgtcagt gacgcgctgc tcccatcttt ctccacgttc
     3601 gcgtctggcc cggcgggaag ggagaagaca ctgcgtcaag caggtgcccc gaataaccgc
     3661 tggcgggagg agctctccca catgaagcga cttcccccag tgcttcccgg ccgcccctat
     3721 gacctggcgg cggcgaccgt ggccacagac ctggagagcg gcggagccgg tgcggcttgc
     3781 ggcggtagca acctggcgcc cctacctcgg agagagaccg aggagttcaa cgatctcctg
     3841 gacctggact ttattctctc caattcgctg acccatcctc cggagtcagt ggccgccacc
     3901 gtgtcctcgt cagcgtcagc ctcctcttcg tcgtcgccgt cgagcagcgg ccctgccagc
     3961 gcgccctcca cctgcagctt cacctatccg atccgggccg ggaacgaccc gggcgtggcg
     4021 ccgggcggca cgggcggagg cctcctctat ggcagggagt ccgctccccc tccgacggct
     4081 cccttcaacc tggcggacat caacgacgtg agcccctcgg gcggcttcgt ggccgagctc
     4141 ctgcggccag aattggaccc ggtgtacatt ccgccgcagc agccgcagcc gccaggtggc
     4201 gggctgatgg gcaagttcgt gctgaaggcg tcgctgagcg cccctggcag cgagtacggc
     4261 agcccgtcgg tcatcagcgt cagcaaaggc agccctgacg gcagccaccc ggtggtggtg
     4321 gcgccctaca acggcgggcc gccgcgcacg tgccccaaga tcaagcagga ggcggtctct
     4381 tcgtgcaccc acttgggcgc tggaccccct ctcagcaatg gccaccggcc ggctgcacac
     4441 gacttccccc tggggcggca gctccccagc aggactaccc cgaccctggg tcttgaggaa
     4501 gtgctgagca gcagggactg tcaccctgcc ctgccgcttc ctcccggctt ccatccccac
     4561 ccggggccca attacccatc cttcctgccc gatcagatgc agccgcaagt cccgccgctc
     4621 cattaccaag agctcatgcc acccggttcc tgcatgccag aggagcccaa gccaaagagg
     4681 ggaagacgat cgtggccccg gaaaaggacc gccacccaca cttgtgatta cgcgggctgc
     4741 ggcaaaacct acacaaagag ttcccatctc aaggcacacc tgcgaaccca cacaggtgag
     4801 aaaccttacc actgtgactg ggacggctgt ggatggaaat tcgcccgctc agatgaactg
     4861 accaggcact accgtaaaca cacggggcac cgcccgttcc agtgccaaaa atgcgaccga
     4921 gcattttcca ggtcggacca cctcgcctta cacatgaaga gacattttta aaagggcgaa
     4981 ttgcctgcag gaattcaagc ttcggggact agtcatatga taatcaacct ctggattaca
     5041 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5101 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5161 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5221 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5281 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5341 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5401 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     5461 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5521 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5581 gtcggatctc cctttgggcc gcctccccgc atcggtacaa ttcactcctc aggtgcaggc
     5641 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga
     5701 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     5761 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     5821 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     5881 gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg
     5941 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac
     6001 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa
     6061 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat
     6121 agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat
     6181 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6241 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6301 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atctcaattc
     6361 cgatcatatt caataaccct taatataact tcgtataatg tatgctatac gaagttatta
     6421 ggtctgaaga ggagtttacg tccagccaag cttaggatca attctcatgt ttgacagctt
     6481 atcatcgata agctgatcct cacaggccgc acccagcttt tcttccgttg ccccagtagc
     6541 atctctgtct ggtgaccttg aagaggaaga ggaggggtcc cgagaatccc catccctacc
     6601 gtccagcaaa aagggggacg aggaatttga ggcctggctt gaggctcagg acgcaaatct
     6661 tgaggatgtt cagcgggagt tttccgggct gcgagtaatt ggtgatgagg acgaggatgg
     6721 ttcggaggat ggggaatttt cagacctgga tctgtctgac agcgaccatg aaggggatga
     6781 gggtgggggg gctgttggag ggggcaggag tctgcactcc ctgtattcac tgagcgtcgt
     6841 ctaataaaga tgtctattga tctcttttag tgtgaatcat gtctgacgag gggccaggta
     6901 caggacctgg aaatggccta ggagagaagg gagacacatc tggaccagaa ggctccggcg
     6961 gcagtggacc tcaaagaaga gggggtgata accatggacg aggacgggga agaggacgag
     7021 gacgaggagg cggaagacca ggagccccgg gcggctcagg atcagggcca agacatagag
     7081 atggtgtccg gagaccccaa aaacgtccaa gttgcattgg ctgcaaaggg acccacggtg
     7141 gaacaggagc aggagcagga gcgggagggg caggagcagg aggggcagga gcaggaggag
     7201 gggcaggagc aggaggaggg gcaggagggg caggaggggc aggaggggca ggagcaggag
     7261 gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggagca ggaggagggg
     7321 caggagcagg aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag
     7381 gggcaggagc aggaggaggg gcaggagcag gaggaggggc aggaggggca ggagcaggag
     7441 gaggggcagg aggggcagga ggggcaggag caggaggagg ggcaggagca ggaggggcag
     7501 gaggggcagg aggggcagga gcaggagggg caggagcagg aggaggggca ggaggggcag
     7561 gaggggcagg agcaggaggg gcaggagcag gaggggcagg agcaggaggg gcaggagcag
     7621 gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggag
     7681 gggcaggagc aggaggaggg gcaggagggg caggagcagg aggaggggca ggaggggcag
     7741 gagcaggagg ggcaggaggg gcaggagcag gaggggcagg aggggcagga gcaggagggg
     7801 caggaggggc aggagcagga ggaggggcag gagcaggagg ggcaggagca ggaggtggag
     7861 gccggggtcg aggaggcagt ggaggccggg gtcgaggagg tagtggaggc cggggtcgag
     7921 gaggtagtgg aggccgccgg ggtagaggac gtgaaagagc caggggggga agtcgtgaaa
     7981 gagccagggg gagaggtcgt ggacgtggag aaaagaggcc caggagtccc agtagtcagt
     8041 catcatcatc cgggtctcca ccgcgcaggc cccctccagg tagaaggcca tttttccacc
     8101 ctgtagggga agccgattat tttgaatacc accaagaagg tggcccagat ggtgagcctg
     8161 acgtgccccc gggagcgata gagcagggcc ccgcagatga cccaggagaa ggcccaagca
     8221 ctggaccccg gggtcagggt gatggaggca ggcgcaaaaa aggagggtgg tttggaaagc
     8281 atcgtggtca aggaggttcc aacccgaaat ttgagaacat tgcagaaggt ttaagagctc
     8341 tcctggctag gagtcacgta gaaaggacta ccgacgaagg aacttgggtc gccggtgtgt
     8401 tcgtatatgg aggtagtaag acctcccttt acaacctaag gcgaggaact gcccttgcta
     8461 ttccacaatg tcgtcttaca ccattgagtc gtctcccctt tggaatggcc cctggacccg
     8521 gcccacaacc tggcccgcta agggagtcca ttgtctgtta tttcatggtc tttttacaaa
     8581 ctcatatatt tgctgaggtt ttgaaggatg cgattaagga ccttgttatg acaaagcccg
     8641 ctcctacctg caatatcagg gtgactgtgt gcagctttga cgatggagta gatttgcctc
     8701 cctggtttcc acctatggtg gaaggggctg ccgcggaggg tgatgacgga gatgacggag
     8761 atgaaggagg tgatggagat gagggtgagg aagggcagga gtgatgtaac ttgttaggag
     8821 acgccctcaa tcgtattaaa agccgtgtat tcccccgcac taaagaataa atccccagta
     8881 gacatcatgc gtgctgttgg tgtatttctg gccatctgtc ttgtcaccat tttcgtcctc
     8941 ccaacatggg gcaattgccg gaacccttaa tataacttcg tataatgtat gctatacgaa
     9001 gttattaggt ccctcgaaga ggttcactag cggatctcaa ttgggcatac ccatgttgtc
     9061 acgtcactca gctccgcgct caacaccttc tcgcgttgga aaacattagc gacatttacc
     9121 tggtgagcaa tcagacatgc gacggcttta gcctggcctc cttaaattca cctaagaatg
     9181 ggagcaacca gcaggaaaag gacaagcagc gaaaattcac gcccccttgg gaggtggcgg
     9241 catatgcaaa ggatagcact cccactctac tactgggtat catatgctga ctgtatatgc
     9301 atgaggatag catatgctac ccggatacag attaggatag catatactac ccagatatag
     9361 attaggatag catatgctac ccagatatag attaggatag cctatgctac ccagatataa
     9421 attaggatag catatactac ccagatatag attaggatag catatgctac ccagatatag
     9481 attaggatag cctatgctac ccagatatag attaggatag catatgctac ccagatatag
     9541 attaggatag catatgctat ccagatattt gggtagtata tgctacccag atataaatta
     9601 ggatagcata tactacccta atctctatta ggatagcata tgctacccgg atacagatta
     9661 ggatagcata tactacccag atatagatta ggatagcata tgctacccag atatagatta
     9721 ggatagccta tgctacccag atataaatta ggatagcata tactacccag atatagatta
     9781 ggatagcata tgctacccag atatagatta ggatagccta tgctacccag atatagatta
     9841 ggatagcata tgctatccag atatttgggt agtatatgct acccatggca acattagccc
     9901 accgtgctct cagcgacctc gtgaatatga ggaccaacaa ccctgtgctt ggcgctcagg
     9961 cgcaagtgtg tgtaatttgt cctccagatc gcagcaatcg cgcccctatc ttggcccgcc
    10021 cacctactta tgcaggtatt ccccggggtg ccattagtgg ttttgtgggc aagtggtttg
    10081 accgcagtgg ttagcggggt tacaatcagc caagttatta cacccttatt ttacagtcca
    10141 aaaccgcagg gcggcgtgtg ggggctgacg cgtgccccca ctccacaatt tcaaaaaaaa
    10201 gagtggccac ttgtctttgt ttatgggccc cattggcgtg gagccccgtt taattttcgg
    10261 gggtgttaga gacaaccagt ggagtccgct gctgtcggcg tccactctct ttccccttgt
    10321 tacaaataga gtgtaacaac atggttcacc tgtcttggtc cctgcctggg acacatctta
    10381 ataaccccag tatcatattg cactaggatt atgtgttgcc catagccata aattcgtgtg
    10441 agatggacat ccagtcttta cggcttgtcc ccaccccatg gatttctatt gttaaagata
    10501 ttcagaatgt ttcattccta cactagtatt tattgcccaa ggggtttgtg agggttatat
    10561 tggtgtcata gcacaatgcc accactgaac cccccgtcca aattttattc tgggggcgtc
    10621 acctgaaacc ttgttttcga gcacctcaca tacaccttac tgttcacaac tcagcagtta
    10681 ttctattagc taaacgaagg agaatgaaga agcaggcgaa gattcaggag agttcactgc
    10741 ccgctccttg atcttcagcc actgcccttg tgactaaaat ggttcactac cctcgtggaa
    10801 tcctgacccc atgtaaataa aaccgtgaca gctcatgggg tgggagatat cgctgttcct
    10861 taggaccctt ttactaaccc taattcgata gcatatgctt cccgttgggt aacatatgct
    10921 attgaattag ggttagtctg gatagtatat actactaccc gggaagcata tgctacccgt
    10981 ttagggttaa caagggggcc ttataaacac tattgctaat gccctcttga gggtccgctt
    11041 atcggtagct acacaggccc ctctgattga cgttggtgta gcctcccgta gtcttcctgg
    11101 gcccctggga ggtacatgtc ccccagcatt ggtgtaagag cttcagccaa gagttacaca
    11161 taaaggcaat gttgtgttgc agtccacaga ctgcaaagtc tgctccagga tgaaagccac
    11221 tcagtgttgg caaatgtgca catccattta taaggatgtc aactacagtc agagaacccc
    11281 tttgtgtttg gtcccccccc gtgtcacatg tggaacaggg cccagttggc aagttgtacc
    11341 aaccaactga agggattaca tgcactgccc cgcgaagaag gggcagagat gtcgtagtca
    11401 ggtttagttc gtccggggcg gggatcgatc ctctagagtc gacgctagcg gatccgctgc
    11461 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    11521 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    11581 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    11641 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    11701 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    11761 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    11821 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    11881 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    11941 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    12001 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    12061 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    12121 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    12181 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    12241 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    12301 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    12361 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    12421 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    12481 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    12541 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    12601 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    12661 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    12721 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    12781 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    12841 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    12901 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    12961 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    13021 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    13081 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    13141 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    13201 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    13261 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    13321 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    13381 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    13441 ctgg
//
