LOCUS Exported 5811 bp ds-DNA circular SYN 15-DEC-2022 DEFINITION Retroviral vector expressing human SOX2-17 chimeric transcription factor (super-SOX). ACCESSION . VERSION . KEYWORDS pMXs-hSOX2-17 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5811) AUTHORS MacCarthy CM, Malik V, Wu G, Velychko T, Keshet G, Jauch R, Cojocaru V, Scholer HR, Velychko S TITLE Enhancing Sox/Oct cooperativity induces higher-grade developmental reset JOURNAL bioRxiv 2022.09.23.509242 REFERENCE 2 (bases 1 to 5811) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 15, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5811 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 22..615 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 678..1035 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1094..1510 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1440..1462 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1478..1494 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" misc_feature 1520..1894 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" primer_bind 1832..1848 /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" CDS 2820..2837 /codon_start=1 /product="HQHQHQ metal affinity tag" /label=HQ tag /note="The HQ tag binds to nickel and other metal ions much like the 6xHis tag, but may allow elution at lower concentrations of imidazole (Godat et al., 2008)." /translation="HQHQHQ" CDS 2826..2843 /codon_start=1 /product="HQHQHQ metal affinity tag" /label=HQ tag /note="The HQ tag binds to nickel and other metal ions much like the 6xHis tag, but may allow elution at lower concentrations of imidazole (Godat et al., 2008)." /translation="HQHQHQ" LTR 3316..3785 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(3915..3932) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4086..4674) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4166..4185) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4838..5698) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5461..5480 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5699..5803) /gene="bla" /label=AmpR promoter ORIGIN 1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta 61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc 121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag 181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat 241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg 301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct 361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg 421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc 481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc 541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag 601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg 661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg 721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg 781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc 841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg 901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct 961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc 1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt 1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa 1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac 1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa 1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc 1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt 1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc 1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc 1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa 1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca 1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca 1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg 1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac 1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc 1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat 1921 cccagtgtgg tggtacggga attcgtttaa acatgtacaa catgatggag acggagctga 1981 agccgccggg cccgcagcaa acttcggggg gcggcggcgg caactccacc gcggcggcgg 2041 ccggcggcaa ccagaaaaac agcccggacc gcgtcaagcg gcccatgaat gccttcatgg 2101 tgtggtcccg cgggcagcgg cgcaagatgg cccaggagaa ccccaagatg cacaactcgg 2161 agatcagcaa gcgcctgggc gccgagtgga aagcgctgac gctggcggag aagcggccgt 2221 tcatcgacga ggctaagcgg ctgcgagtgc tgcacatgca ggaccacccc aactacaagt 2281 accggccgcg gcggcgcaag caggtgaagc ggctgaagcg ggtggagggc ggcttcctgc 2341 acggcctggc tgagccgcag gcggccgcgc tgggccccga gggcggccgc gtggccatgg 2401 acggcctggg cctccagttc cccgagcagg gcttccccgc cggcccgccg ctgctgcctc 2461 cgcacatggg cggccactac cgcgactgcc agagtctggg cgcgcctccg ctcgacggct 2521 acccgttgcc cacgcccgac acgtccccgc tggacggcgt ggaccccgac ccggctttct 2581 tcgccgcccc gatgcccggg gactgcccgg cggccggcac ctacagctac gcgcaggtct 2641 cggactacgc tggccccccg gagcctcccg ccggtcccat gcacccccga ctcggcccag 2701 agcccgcggg tccctcgatt ccgggcctcc tggcgccacc cagcgccctt cacgtgtact 2761 acggcgcgat gggctcgccc ggggcgggcg gcgggcgcgg cttccagatg cagccgcaac 2821 accagcacca gcaccagcac cagcaccacc ccccgggccc cggacagccg tcgccccctc 2881 cggaggcact gccctgccgg gacggcacgg accccagtca gcccgccgag ctcctcgggg 2941 aggtggaccg cacggaattt gaacagtatc tgcacttcgt gtgcaagcct gagatgggcc 3001 tcccctacca ggggcatgac tccggtgtga atctccccga cagccacggg gccatttcct 3061 cggtggtgtc cgacgccagc tccgcggtat attactgcaa ctatcctgac gtgtgaacta 3121 gtctcgagcg gccgccagca cagtggtcga cgataaaata aaagatttta tttagtctcc 3181 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc 3241 cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag 3301 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc 3361 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg 3421 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc 3481 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac 3541 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg 3601 ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg 3661 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt 3721 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc 3781 tttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa 3841 tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 3901 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 3961 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 4021 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 4081 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 4141 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 4201 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 4261 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 4321 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 4381 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 4441 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 4501 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 4561 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 4621 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 4681 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 4741 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgcggcc ggccgcaaat 4801 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 4861 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 4921 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 4981 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 5041 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 5101 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 5161 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 5221 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 5281 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 5341 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 5401 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 5461 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 5521 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 5581 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 5641 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 5701 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 5761 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt c //