LOCUS Exported 14537 bp ds-DNA circular SYN 15-DEC-2022 DEFINITION episomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCs. ACCESSION . VERSION . KEYWORDS pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14537) AUTHORS MacCarthy CM, Malik V, Wu G, Velychko T, Keshet G, Jauch R, Cojocaru V, Scholer HR, Velychko S TITLE Enhancing Sox/Oct cooperativity induces higher-grade developmental reset JOURNAL bioRxiv 2022.09.23.509242 REFERENCE 2 (bases 1 to 14537) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 15, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..14537 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 26..614 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(79..99) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(497..508) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(705..724) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 770..825 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(824..843) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(1186..1202) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1186..1202) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1199..1221) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1210..1226 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1234..1264) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1279..1300 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." protein_bind complement(1389..1422) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS 1883..3808 /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /label=EBNA1 /note="crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGA GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG GGAGGAGAGGGGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" protein_bind complement(3976..4009) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." rep_origin 4196..5985 /label=oriP /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" primer_bind complement(6526..6543) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6697..7285) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6777..6796) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7456..8316) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8079..8098 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8317..8421) /gene="bla" /label=AmpR promoter enhancer 8452..8831 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 8833..9110 /label=chicken beta-actin promoter intron 9111..10127 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 10051..10073 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 10135..10154 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" CDS 11269..11325 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" misc_feature 12754..13321 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 12771..13323 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(12915..12932) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 13142..13161 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" ORIGIN 1 aagcttcggg gactagtcat atgataatca acctctggat tacaaaattt gtgaaagatt 61 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 121 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 181 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 241 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 301 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 361 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 421 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 481 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 541 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg 601 ggccgcctcc ccgcatcggt acaattcact cctcaggtgc aggctgccta tcagaaggtg 661 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc 721 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa 781 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat 841 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat 901 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa 961 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt 1021 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt 1081 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc 1141 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc 1201 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 1261 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 1321 cgctttccag tcgggaaacc tgtcgtgcca gcggatctca attccgatca tattcaataa 1381 cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtctg aagaggagtt 1441 tacgtccagc caagcttagg atcaattctc atgtttgaca gcttatcatc gataagctga 1501 tcctcacagg ccgcacccag cttttcttcc gttgccccag tagcatctct gtctggtgac 1561 cttgaagagg aagaggaggg gtcccgagaa tccccatccc taccgtccag caaaaagggg 1621 gacgaggaat ttgaggcctg gcttgaggct caggacgcaa atcttgagga tgttcagcgg 1681 gagttttccg ggctgcgagt aattggtgat gaggacgagg atggttcgga ggatggggaa 1741 ttttcagacc tggatctgtc tgacagcgac catgaagggg atgagggtgg gggggctgtt 1801 ggagggggca ggagtctgca ctccctgtat tcactgagcg tcgtctaata aagatgtcta 1861 ttgatctctt ttagtgtgaa tcatgtctga cgaggggcca ggtacaggac ctggaaatgg 1921 cctaggagag aagggagaca catctggacc agaaggctcc ggcggcagtg gacctcaaag 1981 aagagggggt gataaccatg gacgaggacg gggaagagga cgaggacgag gaggcggaag 2041 accaggagcc ccgggcggct caggatcagg gccaagacat agagatggtg tccggagacc 2101 ccaaaaacgt ccaagttgca ttggctgcaa agggacccac ggtggaacag gagcaggagc 2161 aggagcggga ggggcaggag caggaggggc aggagcagga ggaggggcag gagcaggagg 2221 aggggcagga ggggcaggag gggcaggagg ggcaggagca ggaggagggg caggagcagg 2281 aggaggggca ggaggggcag gaggggcagg agcaggagga ggggcaggag caggaggagg 2341 ggcaggaggg gcaggagcag gaggaggggc aggaggcgca ggaggggcag gagcaggagg 2401 aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggagggg caggaggggc 2461 aggaggggca ggagcaggag gaggggcagg agcaggaggg gcaggagggg caggaggggc 2521 aggagcagga ggggcaggag caggggcagg agcaggaggg gcaggagggg caggagcagg 2581 aggggcagga gcaggagggg caggagcagg aggggcagga gcaggagggg caggaggggc 2641 aggagcagga ggggcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg 2701 aggggcagga ggggcaggag caggaggagg ggcaggaggg gcaggagcag gagggggagg 2761 aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc 2821 aggaggaggg gcaggagcag gaggggcagg agcaggaggt ggaggccggg gtcgaggagg 2881 cagtggaggc cggggtcgag gaggtagtgg aggccggggt cgaggaggta gtggaggccg 2941 ccggggtaga ggacgtgaaa gagccagggg gggaagtcgt gaaagagcca gggggagagg 3001 tcgtggacgt ggagaaaaga ggcccaggag tcccagtagt cagtcatcat catccgggtc 3061 tccaccgcgc aggccccctc caggtagaag gccatttttc caccctgtag gggaagccga 3121 ttattttgaa taccaccaag aaggtggccc agatggtgag cctgacgtgc ccccgggagc 3181 gatagagcag ggccccgcag atgacccagg agaaggccca agcactggac cccggggtca 3241 gggtgatgga ggcaggcgca aaaaaggagg gtggtttgga aagcatcgtg gtcaaggagg 3301 ttccaacccg aaatttgaga acattgcaga aggtttaaga gctctcctgg ctaggagtca 3361 cgtagaaagg actaccgacg aaggaacttg ggtcgccggt gtgttcgtat atggaggtag 3421 taagacctcc ctttacaacc taaggcgagg aactgccctt gctattccac aatgtcgtct 3481 tacaccattg agtcgtctcc cctttggaat ggcccctgga cccggcccac aacctggccc 3541 gctaagggag tccattgtct gttatttcat ggtcttttta caaactcata tatttgctga 3601 ggttttgaag gatgcgatta aggaccttgt tatgacaaag cccgctccta cctgcaatat 3661 cagggtgact gtgtgcagct ttgacgatgg agtagatttg cctccctggt ttccacctat 3721 ggtggaaggg gctgccgcgg agggtgatga cggagatgac ggagatgaag gaggtgatgg 3781 agatgagggt gaggaagggc aggagtgatg taacttgtta ggagacgccc tcaatcgtat 3841 taaaagccgt gtattccccc gcactaaaga ataaatcccc agtagacatc atgcgtgctg 3901 ttggtgtatt tctggccatc tgtcttgtca ccattttcgt cctcccaaca tggggcaatt 3961 gccggaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt aggtccctcg 4021 aagaggttca ctagcggatc tcaattgggc atacccatgt tgtcacgtca ctcagctccg 4081 cgctcaacac cttctcgcgt tggaaaacat tagcgacatt tacctggtga gcaatcagac 4141 atgcgacggc tttagcctgg cctccttaaa ttcacctaag aatgggagca accagcagga 4201 aaaggacaag cagcgaaaat tcacgccccc ttgggaggtg gcggcatatg caaaggatag 4261 cactcccact ctactactgg gtatcatatg ctgactgtat atgcatgagg atagcatatg 4321 ctacccggat acagattagg atagcatata ctacccagat atagattagg atagcatatg 4381 ctacccagat atagattagg atagcctatg ctacccagat ataaattagg atagcatata 4441 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg 4501 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcatatg 4561 ctatccagat atttgggtag tatatgctac ccagatataa attaggatag catatactac 4621 cctaatctct attaggatag catatgctac ccggatacag attaggatag catatactac 4681 ccagatatag attaggatag catatgctac ccagatatag attaggatag cctatgctac 4741 ccagatataa attaggatag catatactac ccagatatag attaggatag catatgctac 4801 ccagatatag attaggatag cctatgctac ccagatatag attaggatag catatgctat 4861 ccagatattt gggtagtata tgctacccat ggcaacatta gcccaccgtg ctctcagcga 4921 cctcgtgaat atgaggacca acaaccctgt gcttggcgct caggcgcaag tgtgtgtaat 4981 ttgtcctcca gatcgcagca atcgcgcccc tatcttggcc cgcccaccta cttatgcagg 5041 tattccccgg ggtgccatta gtggttttgt gggcaagtgg tttgaccgca gtggttagcg 5101 gggttacaat cagccaagtt attacaccct tattttacag tccaaaaccg cagggcggcg 5161 tgtgggggct gacgcgtgcc cccactccac aatttcaaaa aaaagagtgg ccacttgtct 5221 ttgtttatgg gccccattgg cgtggagccc cgtttaattt tcgggggtgt tagagacaac 5281 cagtggagtc cgctgctgtc ggcgtccact ctctttcccc ttgttacaaa tagagtgtaa 5341 caacatggtt cacctgtctt ggtccctgcc tgggacacat cttaataacc ccagtatcat 5401 attgcactag gattatgtgt tgcccatagc cataaattcg tgtgagatgg acatccagtc 5461 tttacggctt gtccccaccc catggatttc tattgttaaa gatattcaga atgtttcatt 5521 cctacactag tatttattgc ccaaggggtt tgtgagggtt atattggtgt catagcacaa 5581 tgccaccact gaaccccccg tccaaatttt attctggggg cgtcacctga aaccttgttt 5641 tcgagcacct cacatacacc ttactgttca caactcagca gttattctat tagctaaacg 5701 aaggagaatg aagaagcagg cgaagattca ggagagttca ctgcccgctc cttgatcttc 5761 agccactgcc cttgtgacta aaatggttca ctaccctcgt ggaatcctga ccccatgtaa 5821 ataaaaccgt gacagctcat ggggtgggag atatcgctgt tccttaggac ccttttacta 5881 accctaattc gatagcatat gcttcccgtt gggtaacata tgctattgaa ttagggttag 5941 tctggatagt atatactact acccgggaag catatgctac ccgtttaggg ttaacaaggg 6001 ggccttataa acactattgc taatgccctc ttgagggtcc gcttatcggt agctacacag 6061 gcccctctga ttgacgttgg tgtagcctcc cgtagtcttc ctgggcccct gggaggtaca 6121 tgtcccccag cattggtgta agagcttcag ccaagagtta cacataaagg caatgttgtg 6181 ttgcagtcca cagactgcaa agtctgctcc aggatgaaag ccactcagtg ttggcaaatg 6241 tgcacatcca tttataagga tgtcaactac agtcagagaa cccctttgtg tttggtcccc 6301 ccccgtgtca catgtggaac agggcccagt tggcaagttg taccaaccaa ctgaagggat 6361 tacatgcact gccccgcgaa gaaggggcag agatgtcgta gtcaggttta gttcgtccgg 6421 ggcggggatc gatcctctag agtcgacgct agcggatccg ctgcattaat gaatcggcca 6481 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 6541 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 6601 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 6661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 6721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 6781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 6841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 6901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 6961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 7141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 7201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 7261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 7321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 7381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 7441 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 7501 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 7561 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 7621 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 7681 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 7741 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 7801 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 7861 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 7921 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 7981 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 8041 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 8101 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 8161 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 8221 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 8281 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 8341 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 8401 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt cgacattgat 8461 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 8521 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 8581 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 8641 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 8701 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 8761 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 8821 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct 8881 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg 8941 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga 9001 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg 9061 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg 9121 cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct 9181 gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta 9241 attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg 9301 ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt 9361 gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg 9421 gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc 9481 cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg 9541 ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc 9601 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc 9661 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc 9721 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag 9781 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc 9841 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg 9901 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg 9961 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg 10021 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct 10081 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg 10141 tgctggttat tgtgctgtct catcattttg gcaaagaatt cgcggccgcg tttaaacatg 10201 gctggacacc tggcttcaga cttcgccttc tcacccccac caggtggggg tgatgggtca 10261 gcagggctgg agccgggctg ggtggattct cgaacctggc taagcttcca agggcctcca 10321 ggtgggcctg gaatcggacc aggctcagag gtattgggga tctccccatg tccgcccgca 10381 tacgagttct gcggagggat ggcatactgt ggacctcagg ttggactggg cctagtcccc 10441 caagttggcg tggagacttt gcagcctgag ggccaggcag gagcacgagt ggaaagcaac 10501 tcagagggaa cctcctctga gccctgtgcc gaccgcccca atgccgtgaa gttggagaag 10561 gtggaaccaa ctcccgagga gtcccaggac atgaaagccc tgcagaagga gctagaacag 10621 tttgccaagc tgctgaagca gaagaggatc accttggggt acacccaggc cgacgtgggg 10681 ctcaccctgg gcgttctctt tggaaaggtg ttcagccaga ccaccatctg tcgcttcgag 10741 gccttgcagc tcagccttaa gaacatgtgt aagctgcggc ccctgctgga gaagtgggtg 10801 gaggaagccg acaacaatga gaaccttcag gagatatgca aatcggagac cctggtgcag 10861 gcccggaaga gaaagcgaac tagcattgag aaccgtgtga ggtggagtct ggagaccatg 10921 tttctgaagt gcccgaagcc ctccctacag cagatcactc acatcgccaa tcagcttggg 10981 ctagagaagg atgtggttcg agtatggttc tgtaaccggc gccagaaggg caaaagatca 11041 agtattgagt attcccaacg agaagagtat gaggctacag ggacaccttt cccagggggg 11101 gctgtatcct ttcctctgcc cccaggtccc cactttggca ccccaggcta tggaagcccc 11161 cacttcacca cactctactc agtccctttt cctgagggcg aggcctttcc ctctgttccc 11221 gtcactgctc tgggctctcc catgcattca aacacgcgtg gatctggagc caccaacttt 11281 agtctcctca aacaagccgg tgacgttgaa gaaaatccag gccccatggc tgtcagcgac 11341 gctctgctcc cgtccttctc cacgttcgcg tccggcccgg cgggaaggga gaagacactg 11401 cgtccagcag gtgccccgac taaccgttgg cgtgaggaac tctctcacat gaagcgactt 11461 cccccacttc ccggccgccc ctacgacctg gcggcgacgg tggccacaga cctggagagt 11521 ggcggagctg gtgcagcttg cagcagtaac aacccggccc tcctagcccg gagggagacc 11581 gaggagttca acgacctcct ggacctagac tttatccttt ccaactcgct aacccaccag 11641 gaatcggtgg ccgccaccgt gaccacctcg gcgtcagctt catcctcgtc ttccccagcg 11701 agcagcggcc ctgccagcgc gccctccacc tgcagcttca gctatccgat ccgggccggg 11761 ggtgacccgg gcgtggctgc cagcaacaca ggtggagggc tcctctacag ccgagaatct 11821 gcgccacctc ccacggcccc cttcaacctg gcggacatca atgacgtgag cccctcgggc 11881 ggcttcgtgg ctgagctcct gcggccggag ttggacccag tatacattcc gccacagcag 11941 cctcagccgc caggtggcgg gctgatgggc aagtttgtgc tgaaggcgtc tctgaccacc 12001 cctggcagcg agtacagcag cccttcggtc atcagtgtta gcaaaggaag cccagacggc 12061 agccaccccg tggtagtggc gccctacagc ggtggcccgc cgcgcatgtg ccccaagatt 12121 aagcaagagg cggtcccgtc ctgcacggtc agccggtccc tagaggccca tttgagcgct 12181 ggaccccagc tcagcaacgg ccaccggccc aacacacacg acttccccct ggggcggcag 12241 ctccccacca ggactacccc tacactgagt cccgaggaac tgctgaacag cagggactgt 12301 caccctggcc tgcctcttcc cccaggattc catccccatc cggggcccaa ctaccctcct 12361 ttcctgccag accagatgca gtcacaagtc ccctctctcc attatcaaga gctcatgcca 12421 ccgggttcct gcctgccaga ggagcccaag ccaaagaggg gaagaaggtc gtggccccgg 12481 aaaagaacag ccacccacac ttgtgactat gcaggctgtg gcaaaaccta taccaagagt 12541 tctcatctca aggcacacct gcgaactcac acaggcgaga aaccttacca ctgtgactgg 12601 gacggctgtg ggtggaaatt cgcccgctcg gatgaactga ccaggcacta ccgcaaacac 12661 acagggcacc ggccctttca gtgccagaag tgcgacaggg ccttttccag gtcggaccac 12721 cttgccttac acatgaagag gcacttttaa agatccctcc ccccccccta acgttactgg 12781 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt 12841 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc 12901 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc 12961 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg 13021 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc 13081 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa 13141 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg 13201 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa 13261 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata 13321 atatggccac acatatgatg tataacatga tggagacgga gctgaagccg ccgggcccgc 13381 agcaagcttc ggggggcggc ggcggaggag gcaacgccac ggcggcggca accggcggca 13441 accagaagaa cagcccggac cgcgtcaaga ggcccatgaa cgccttcatg gtatggtccc 13501 gggggcagcg gcgtaagatg gcccaggaga accccaagat gcacaactcg gagatcagca 13561 agcgcctggg cgcggagtgg aaagcgttga ccttggcaga gaagcggccg ttcatcgacg 13621 aggccaagcg gctgcgcgtg ctgcatatgc aggaccaccc caactacaag taccggccgc 13681 ggcggcgcaa gcaggtgaag cgcatgaagc gggtggaggg aggcttcctg cacgctctcg 13741 tcgagcccca ggccggcgcg cttggtcccg agggcggccg cgtggccatg gatggcctgg 13801 gtctgccttt cccggagccg ggctatccgg ccggtcctcc gctgatgtct ccgcacatgg 13861 gcccccacta tcgggactgc cagggactgg gcgctcccgc gctcgacggc taccctctgc 13921 ccactccgga cacatccccg ctggatggcg tggagcagga cccggctttc tttgcagccc 13981 cgctgccagg ggactgcccg gcggccggca cctacactta cgctccagtc tcggactatg 14041 cagtgtccgt agagccgccc gctggcccca tgcgagtggg gccggacccc tcgggccctg 14101 cgatgccggg gatcctggcg ccccccagcg ctctgcacct gtactacggc gcgatgggct 14161 cgcccgccgc aagtgcgggg cgcggtttcc acgcgcaacc ccagcagccg ctgcaaccgc 14221 aggcaccgcc cccgccaccg cagcagcagc acccagcgca cggccccggg caaccttcgc 14281 cccctcccga ggctctgccc tgccgggatg gcacggaatc caaccagccc actgagctcc 14341 taggggaggt ggaccgcacg gaatttgaac agtatctgcc ctttgtgtat aagcccgaga 14401 tgggtcttcc ctaccaggga cacgactgcg gagtgaacct ctcagacagc cacggagcca 14461 tttcctccgt ggtgtccgac gctagctcag cggtctacta ttgcaactac cccgacattt 14521 gataaatcga agaattc //