LOCUS       Exported                4332 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pGEMTEZ-Kir2.1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Yu CR, Power J, Barnea G, O'Donnell S, Brown HE, Osborne J, Axel R, 
            Gogos JA
  TITLE     Spontaneous neural activity is required for the establishment and 
            maintenance of the olfactory sensory map.
  JOURNAL   Neuron. 2004 May 27;42(4):553-66.
  PUBMED    15157418
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      33..42
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      37..46
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(1457..1475)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1458..1475)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1493..1509)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1493..1509)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1506..1528)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1517..1533
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1541..1571)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1586..1607
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1724..1741)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1895..2483)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1975..1994)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2654..3514)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3277..3296
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3515..3619)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3697..4152)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3834..3855)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4046..4065
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4278..4300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4292..4309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4293..4309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4316..3
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4316..2
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 cgcgcgctcg agaccatggg cagtgtgaga accaaccgct acagcatcgt ctcttcggag
      121 gaagatggca tgaagctggc cactatggca gttgccaatg gctttgggaa tggcaagagt
      181 aaagtccata cccgacaaca gtgcaggagc cgctttgtga agaaagatgg tcattgcaat
      241 gttcagttta tcaacgtggg tgagaaggga cagaggtacc tggcagacat ctttactacc
      301 tgtgtcgaca tccgctggag gtggatgctg gttatcttct gtcttgcctt cgtgctctcc
      361 tggctgttct ttggctgtgt gttttggttg atagccctgc tccatgggga tctagatact
      421 tctaaagtga gcaaagcatg cgtgtcggag gtcaacagct tcacggctgc cttcctcttc
      481 tccatcgaga cccagacaac cattggctat ggtttcaggt gtgtgacaga cgagtgccca
      541 attgctgtct tcatggtggt attccagtca attgtaggct gcatcattga cgccttcatc
      601 attggtgcag tcatggcgaa gatggcaaag ccaaagaaga gaaatgagac tcttgtcttc
      661 agtcacaatg ctgtgattgc catgagggat ggcaaactct gcttgatgtg gagagtgggt
      721 aaccttcgaa agagccacct tgtggaagct catgtccggg cacagcttct caaatctagg
      781 atcacttcag aaggggagta tatccctttg gaccagatag acatcaatgt tggttttgat
      841 agtggaattg accgcatatt tctagtgtcc cccatcacta tcgttcacga aatagatgaa
      901 gacagccctt tatatgactt gagtaagcag gacattgaca atgcagactt tgaaattgtt
      961 gtcatactgg aaggcatggt ggaggcgact gccatgacaa ctcaatgccg gagttcgtat
     1021 ctggccaatg aaattctctg gggtcaccgc tatgagccag tgctctttga agagaaacac
     1081 tactataaag tagactattc aagattccat aagacttatg aagtacctaa cacccccctt
     1141 tgtagtgcca gagacttagc agagaagaaa tacatccttt caaatgcaaa ttcattttgc
     1201 tatgaaaatg aagttgccct aacaagcaaa gaggaagagg aggatagtga gaacggagtc
     1261 ccagagagca caagcacaga ctcacctcct ggcatagatc tccacaacca ggcaagcgta
     1321 cctctagagc ccaggccctt aaggcgagaa tcggagatat gaccctctag acgcgcgatc
     1381 actagtgaat tcgcggccgc ctgcaggtcg accatatggg agagctccca acgcgttgga
     1441 tgcatagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc
     1501 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     1561 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     1621 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     1681 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1741 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1801 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1861 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1921 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1981 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2041 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2101 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2161 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2221 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2281 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2341 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2401 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2461 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2521 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2581 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2641 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2701 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2761 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     2821 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     2881 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     2941 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3001 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3061 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     3121 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     3181 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3241 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3301 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3361 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3421 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3481 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3541 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3601 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata
     3661 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt
     3721 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     3781 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     3841 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     3901 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     3961 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     4021 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     4081 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     4141 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     4201 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     4261 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata
     4321 cgactcacta ta
//
