LOCUS       Exported                 880 bp ds-DNA     linear   SYN 25-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    LPH2782 - Ub-K48R
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 880)
  TITLE     Linda Hicke lab unpublished Ubiquitin constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 25, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             3..230
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGRQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     terminator      343..590
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(359..377)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 ttatgcagat cttcgtcaag acgttaaccg gtaaaaccat aactctagaa gttgaatctt
       61 ccgataccat cgacaacgtt aagtcgaaaa ttcaagacaa ggaaggcatt ccacctgatc
      121 aacaaagatt gatctttgcc ggtaggcagc tcgaggacgg tagaacgctg tctgattaca
      181 acattcagaa ggagtcgacc ttacatcttg tcttaagact aagaggtggt tgaggtacca
      241 agatggcctt tggtgggttg aagaaggaaa aagacagaaa cgacttaatt acctacttga
      301 aaaaagcctg tgagtaaaca ggcccctttt cctttgtcga tatcatgtaa ttagttatgt
      361 cacgcttaca ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac
      421 aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat
      481 ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac
      541 tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc aagcttatcg
      601 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagggtaata
      661 actgatataa ttaaattgaa gctctaattt gtgagtttag tatacatgca tttacttata
      721 atacagtttt ttagttttgc tggccgcatc ttctcaaata tgcttcccag cctgcttttc
      781 tgtaacgttc accctctacc ttagcatccc ttccctttgc aaatagtcct cttccaacaa
      841 taataatgtc agatcctgta gagaccacat catccacggt
//
