LOCUS       Exported                 792 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    LHP585 - Ub-CYC1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Shih SC, Prag G, Francis SA, Sutanto MA, Hurley JH, Hicke L
  TITLE     A ubiquitin-binding motif required for intramolecular 
            monoubiquitylation, the CUE domain.
  JOURNAL   EMBO J. 2003 Mar 17;22(6):1273-81.
  PUBMED    12628920
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             8..235
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     terminator      348..595
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(364..382)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(614..630)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(614..630)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(627..649)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    638..654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(662..692)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    707..728
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 attcattatg cagatcttcg tcaagacgtt aaccggtaaa accataactc tagaagttga
       61 atcttccgat accatcgaca acgttaagtc gaaaattcaa gacaaggaag gcattccacc
      121 tgatcaacaa agattgatct ttgccggtaa gcagctcgag gacggtagaa cgctgtctga
      181 ttacaacatt cagaaggagt cgaccttaca tcttgtctta agactaagag gtggttgagg
      241 taccaagatg gcctttggtg ggttgaagaa ggaaaaagac agaaacgact taattaccta
      301 cttgaaaaaa gcctgtgagt aaacaggccc cttttccttt gtcgatatca tgtaattagt
      361 tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt
      421 tagacaacct gaagtctagg tccctattta tttttttata gttatgttag tattaagaac
      481 gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat
      541 tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcaagct
      601 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      661 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      721 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      781 tgcattaatg aa
//
