LOCUS Exported 4776 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBAD/HisB-PAmKate SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4776) AUTHORS Gunewardene MS, Subach FV, Gould TJ, Penoncello GP, Gudheti MV, Verkhusha VV, Hess ST TITLE Superresolution imaging of multiple fluorescent proteins with highly overlapping emission spectra in living cells. JOURNAL Biophys J. 2011 Sep 21;101(6):1522-8. Epub 2011 Sep 20. PUBMED 21943434 REFERENCE 2 (bases 1 to 4776) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4776 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..285 /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind 208..227 /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" CDS 331..348 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 352..384 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS 388..411 /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /label=Xpress(TM) tag /translation="DLYDDDDK" primer_bind complement(1177..1194) /label=pBAD Reverse /note="For vectors with E. coli araBAD promoter, reverse primer" terminator 1347..1433 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1525..1552 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" promoter 1571..1662 /gene="bla" /label=AmpR promoter CDS 1663..2523 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1881..1900) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2694..3282 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3183..3202 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3436..3453 /label=L4440 /note="L4440 vector, forward primer" misc_feature 3468..3608 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(3534..3553) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3694..3716 /label=pGEX 3' /note="pGEX vectors, reverse primer" CDS complement(3872..4750) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" ORIGIN 1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc 301 taacaggagg aattaaccat ggggggttct catcatcatc atcatcatgg tatggctagc 361 atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatccgagc 421 tcgagatcta tgagcgagct gatcaccgag aacatgcaca tgaagctgta catggagggc 481 accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc 541 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc 601 ctggctacca gcttcatgta cggcagcaag accttcatca accacaccca gggcatcccc 661 gacttctgga agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa 721 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac 781 aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca 841 ctcggctggg aggccaacac cgagatgctg taccccgctg acggcggcct ggaaggcaga 901 ggggacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaactt gaagaccaca 961 tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggaccgc 1021 agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgaggtggct 1081 gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaatta agaattcgaa 1141 gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg 1201 cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga 1261 ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca 1321 tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 1381 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg 1441 gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat 1501 aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc 1561 tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 1621 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 1681 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 1741 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 1801 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 1861 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa 1921 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 1981 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 2041 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 2101 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 2161 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 2221 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 2281 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 2341 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 2401 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 2461 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 2521 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 2581 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 2641 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 2701 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 2761 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 2821 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 2881 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 2941 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 3001 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 3061 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 3121 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 3181 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 3241 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 3301 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 3361 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 3421 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat 3481 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc 3541 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca 3601 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc 3661 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt 3721 caccgtcatc accgaaacgc gcgaggcagc agatcaattc gcgcgcgaag gcgaagcggc 3781 atgcataatg tgcctgtcaa atggacgaag cagggattct gcaaacccta tgctactccg 3841 tcaagccgtc aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca 3901 ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata 3961 cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca 4021 tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta 4081 agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa 4141 catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact 4201 gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca 4261 tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt 4321 ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt 4381 catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc 4441 agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac 4501 gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt cggcaaacaa 4561 attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac 4621 ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg 4681 ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga tcattttgcg 4741 cttcagccat acttttcata ctcccgccat tcagag //