LOCUS       Exported                 797 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pPSmOrange-C1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis JS, Verkhusha VV
  TITLE     A photoswitchable orange-to-far-red fluorescent protein, PSmOrange.
  JOURNAL   Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038/nmeth.1664.
  PUBMED    21804536
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      28..37
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             34..741
                     /codon_start=1
                     /product="orange to far-red photoswitchable variant of the 
                     monomeric fluorescent protein mOrange (Subach et al., 
                     2011)"
                     /label=PSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     641..664
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 agtgaaccgt cagatccgct agcggtcgcc accatggtga gcaagggcga ggagaataat
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcgca tggagggcac cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggctt tcagaccgct
      181 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtcccctctc
      241 ttcacctacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttcaag
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga actacgagga cggcggcgtg
      361 gtgaccgtga cccaggactc ctcactgcag gacggcgagt tcatctacaa ggtgaagatg
      421 cgcggcacca acttcccctc cgacggcccc gtgatgcaga agaagaccat gggctgggag
      481 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caggatgagg
      541 ctgaagctga aggacggcgg ccactacacc tccgaggtca agaccaccta caaggccaag
      601 aagtccgtgc agctgcccgg cgcctacatc gtcggcatca agctggacat cacctcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc gaattctgca
      781 gtcgacggta ccgcggg
//
