LOCUS       Exported                 841 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pPSmOrange-Tubulin
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis JS, Verkhusha VV
  TITLE     A photoswitchable orange-to-far-red fluorescent protein, PSmOrange.
  JOURNAL   Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038/nmeth.1664.
  PUBMED    21804536
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      25..34
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             31..738
                     /codon_start=1
                     /product="orange to far-red photoswitchable variant of the 
                     monomeric fluorescent protein mOrange (Subach et al., 
                     2011)"
                     /label=PSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     638..661
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gaaccgtcag atccgctagc ggtcgccacc atggtgagca agggcgagga gaataatatg
       61 gccatcatca aggagttcat gcgcttcaag gtgcgcatgg agggcaccgt gaacggccac
      121 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggctttca gaccgctaag
      181 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc ccctctcttc
      241 acctacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttcaagctg
      301 tccttccccg agggcttcaa gtgggagcgc gtgatgaact acgaggacgg cggcgtggtg
      361 accgtgaccc aggactcctc actgcaggac ggcgagttca tctacaaggt gaagatgcgc
      421 ggcaccaact tcccctccga cggccccgtg atgcagaaga agaccatggg ctgggaggcc
      481 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcag gatgaggctg
      541 aagctgaagg acggcggcca ctacacctcc gaggtcaaga ccacctacaa ggccaagaag
      601 tccgtgcagc tgcccggcgc ctacatcgtc ggcatcaagc tggacatcac ctcccacaac
      661 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
      721 atggacgagc tgtacaaggg tggaggaggt tccggactca gatctcgagt gcgtgagtgc
      781 atctccatcc acgttggcca ggctggtgtc cagattggca atgcctgctg ggagctctac
      841 t
//
