LOCUS       Exported                6737 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Human V5-IRF3-pcDNA3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Zhu J, Smith K, Hsieh PN, Mburu YK, Chattopadhyay S, Sen GC, Sarkar 
            SN
  TITLE     High-throughput screening for TLR3-IFN regulatory factor 3 signaling
            pathway modulators identifies several antipsychotic drugs as TLR 
            inhibitors.
  JOURNAL   J Immunol. 2010 May 15;184(10):5768-76. Epub 2010 Apr 9.
  PUBMED    20382888
REFERENCE   2  (bases 1 to 6737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             8..49
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        complement(1346..1364)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1347..1364)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1384..1401)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1390..1614
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1660..2088
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1747..1766)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1957..1978
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2097..2117)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2102..2432
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2283..2418
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2345..2364
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2499..3293
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2553..2572)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3163..3182
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3467..3588
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3504..3523)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3558..3577
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3637..3653)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3637..3653)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3650..3672)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3661..3677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3685..3715)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3730..3751
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3868..3885)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4039..4627)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4119..4138)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4798..5658)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5421..5440
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5659..5763)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5838..5857)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6029..6408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6409..6612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6563..6583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6657..6676
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6657..6675
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 caccatgggt aagcctatcc ctaaccctct cctcggtctc gattctacgg gaaccccaaa
       61 gccacggatc ctgccctggc tggtgtcgca gctggacctg gggcaactgg agggcgtggc
      121 ctgggtgaac aagagccgca cgcgcttccg catcccttgg aagcacggcc tacggcagga
      181 tgcacagcag gaggatttcg gaatcttcca ggcctgggcc gaggccactg gtgcatatgt
      241 tcccgggagg gataagccag acctgccaac ctggaagagg aatttccgct ctgccctcaa
      301 ccgcaaagaa gggttgcgtt tagcagagga ccggagcaag gaccctcacg acccacataa
      361 aatctacgag tttgtgaact caggagttgg ggacttttcc cagccagaca cctctccgga
      421 caccaatggt ggaggcagta cttctgatac ccaggaagac attctggatg agttactggg
      481 taacatggtg ttggccccac tcccagatcc gggaccccca agcctggctg tagcccctga
      541 gccctgccct cagcccctgc ggagccccag cttggacaat cccactccct tcccaaacct
      601 ggggccctct gagaacccac tgaagcggct gttggtgccg ggggaagagt gggagttcga
      661 ggtgacagcc ttctaccggg gccgccaagt cttccagcag accatctcct gcccggaggg
      721 cctgcggctg gtggggtccg aagtgggaga caggacgctg cctggatggc cagtcacact
      781 gccagaccct ggcatgtccc tgacagacag gggagtgatg agctacgtga ggcatgtgct
      841 gagctgcctg ggtgggggac tggctctctg gcgggccggg cagtggctct gggcccagcg
      901 gctggggcac tgccacacat actgggcagt gagcgaggag ctgctcccca acagcgggca
      961 tgggcctgat ggcgaggtcc ccaaggacaa ggaaggaggc gtgtttgacc tggggccctt
     1021 cattgtagat ctgattacct tcacggaagg aagcggacgc tcaccacgct atgccctctg
     1081 gttctgtgtg ggggagtcat ggccccagga ccagccgtgg accaagaggc tcgtgatggt
     1141 caaggttgtg cccacgtgcc tcagggcctt ggtagaaatg gcccgggtag ggggtgcctc
     1201 ctccctggag aatactgtgg acctgcacat ttccaacagc cacccactct ccctcacctc
     1261 cgaccagtac aaggcctacc tgcaggactt ggtggagggc atggatttcc agggccctgg
     1321 ggagagctga tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
     1381 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     1441 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     1501 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     1561 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     1621 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     1681 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     1741 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     1801 aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc
     1861 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     1921 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     1981 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg
     2041 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     2101 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa
     2161 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     2221 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     2281 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     2341 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     2401 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     2461 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     2521 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     2581 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     2641 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     2701 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     2761 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     2821 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     2881 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     2941 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     3001 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     3061 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     3121 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     3181 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     3241 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     3301 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     3361 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     3421 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     3481 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3541 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     3601 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     3661 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3721 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3781 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3841 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3901 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3961 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4021 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4081 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4141 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4201 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     4261 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4321 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4381 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4441 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     4501 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4561 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4621 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4681 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4741 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4801 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4861 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4921 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4981 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5041 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5101 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5161 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5221 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5281 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5341 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     5401 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     5461 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5521 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5581 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5641 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5701 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5761 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     5821 ctatggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     5881 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     5941 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     6001 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     6061 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     6121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     6181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggactattta
     6241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     6301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     6361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     6421 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     6481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     6541 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     6601 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
     6661 acgactcact atagggagac ccaagcttgg taccgagctc ggatccacta gtaacggccg
     6721 ccagtgtgct ggaattc
//
