LOCUS       Exported                 880 bp ds-DNA     linear   SYN 10-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pB1H2w2-zif268
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA
  TITLE     A systematic characterization of factors that regulate Drosophila 
            segmentation via a bacterial one-hybrid system.
  JOURNAL   Nucleic Acids Res. 2008 Mar 10. ():.
  PUBMED    18332042
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(479..502)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(512..781)
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGR"
ORIGIN
        1 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
       61 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
      121 tattacgcca ggatcttcag cggagcggga cttgtcggcc tttttctagt ctctagacgt
      181 ccttctgtct taaatggatt ttggtatgcc tcttgcgttc atcactcctg gcaaacttcc
      241 tcccacaaat gtcacaggca aaaggcttct cgcctgtgtg ggtgcggatg tgggtggtaa
      301 ggtggtcact acgactgaag ttacgcatgc agattcgaca ctggaagggc ttctgaccgg
      361 tatggatgcg gatatggcgg gtaagctcat ccgagcgaga aaagcggcga tcgcaggact
      421 cgacagggca agcatatggg cgggtaccat gggggggtgt cttggaaccg gtccggaact
      481 tgtcgtcgtc atccttgtag tccgcggccg cacgaccttc agcaatagcg gtaacggctt
      541 gtaattcagc ggcttcctgc tcttgctgtt cctggcgttc gcgaacgtcg aggatctggt
      601 tgttgatcag accttcttcg atttcgcgca gcgcgattac agtggtttta tcgttttctt
      661 ccggtacgag cgggtccttt ccgcctacct gcatctgacg agcgcgacgc gcggcgacca
      721 gtaccaggtc aaaacggtta ccaattttct ctacagcgtc ctgaacagtt acgcgtgcca
      781 tgggtatatc tccttcttgg agccgagact tacaattgtg ttttttcaaa attcttgcag
      841 gtttcgatgg cttcttgtaa taagctgcgt tgctcctctg
//
