LOCUS Exported 5107 bp ds-DNA circular SYN 14-MAR-2023 DEFINITION Localization: Nucleus/Histones, Excitation: 433, Emission: 475. ACCESSION . VERSION . KEYWORDS CYPet-H2B-6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5107) TITLE Davidson Cyan Fluorescent Proteins REFERENCE 2 (bases 1 to 5107) AUTHORS . TITLE Direct Submission JOURNAL Exported Mar 14, 2023 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5107 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 597..974 /codon_start=1 /gene="HIST1H2BJ" /product="human histone H2B" /label=H2B /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP GELAKHAVSEGTKAITKYTSAK" regulatory 987..996 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 993..1712 /codon_start=1 /product="cyan fluorescent protein optimized for FRET with YPet (Nguyen and Daugherty, 2005)" /label=CyPet /note="mammalian codon-optimized" /translation="MVSKGEELFGGIVPILVELEGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK ANFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITHGMDELYK" primer_bind complement(1299..1318) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" polyA_signal 1835..1956 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1872..1891) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1926..1945 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1963..2418) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2100..2121) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2312..2331 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2445..2549 /gene="bla" /label=AmpR promoter promoter 2551..2908 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2574..2594) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2759..2894 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2821..2840 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2943..3737 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2997..3016) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3607..3626 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3925..3944) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3969..4016 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4345..4933 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4834..4853 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gccaccatgc 601 cagagccagc gaagtctgct cccgccccga aaaagggctc caagaaggcg gtgactaagg 661 cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa ggagagctat tccatctatg 721 tgtacaaggt tctgaagcag gtccaccctg acaccggcat ttcgtccaag gccatgggca 781 tcatgaattc gtttgtgaac gacattttcg agcgcatcgc aggtgaggct tcccgcctgg 841 cgcattacaa caagcgctcg accatcacct ccagggagat ccagacggcc gtgcgcctgc 901 tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg tactaaggcc atcaccaagt 961 acaccagcgc taaggatcca ccggtcgcca ccatggtgag caagggagag gaactgttcg 1021 gcggcatcgt gcccatcctg gtggagctgg agggcgacgt gaacggccac aagttcagcg 1081 tgagcggcga gggcgagggc gacgccacct acggcaagct gaccctgaag ttcatctgca 1141 ccaccggcaa gctgcccgtg ccctggccca ccctggtgac caccctgacc tggggcgtgc 1201 agtgcttcag ccggtacccc gaccacatga agcagcacga cttcttcaag agcgtgatgc 1261 ccgagggcta cgtgcaggag cggaccatct tcttcaagga cgacggcaac tacaagaccc 1321 gggccgaggt gaagttcgag ggcgacaccc tggtgaaccg gatcgagctg aagggcatcg 1381 acttcaagga ggacggcaac atcctgggcc acaagctgga gtacaactac atcagccaca 1441 acgtgtacat caccgccgac aagcagaaga acggcatcaa ggccaacttc aaggcccggc 1501 acaacatcac cgacggcagc gtgcagctgg ccgaccacta ccagcagaac acccccatcg 1561 gcgacggccc cgtgatcctg cccgacaacc actacctgag cacccagagc gccctgagca 1621 aggaccccaa cgagaagcgg gaccacatgg tgctgctgga gttcgtgacc gccgccggca 1681 tcacccacgg catggacgaa ctgtacaaat aaagcggccg cgactctaga tcataatcag 1741 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 1801 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 1861 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 1921 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata 1981 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 2041 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 2101 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 2161 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 2221 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 2281 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 2341 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 2401 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 2461 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 2521 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt 2581 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 2641 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 2701 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 2761 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 2821 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 2881 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc 2941 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 3001 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 3061 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 3121 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 3181 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 3241 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 3301 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 3361 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 3421 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg 3481 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 3541 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 3601 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 3661 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 3721 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa 3781 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 3841 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 3901 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg 3961 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa 4021 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg 4081 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc 4141 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat 4201 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 4261 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 4321 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 4381 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 4441 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 4501 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 4561 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 4621 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 4681 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 4741 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 4801 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 4861 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 4921 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 4981 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 5041 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 5101 agttccg //