LOCUS Exported 5804 bp ds-DNA circular SYN 28-MAR-2023 DEFINITION Express NIR-GECO1 preferentially in neurons. ACCESSION . VERSION . KEYWORDS pAAV-hSyn-NES-NIR-GECO1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5804) AUTHORS Qian Y, Piatkevich KD, Mc Larney B, Abdelfattah AS, Mehta S, Murdock MH, Gottschalk S, Molina RS, Zhang W, Chen Y, Wu J, Drobizhev M, Hughes TE, Zhang J, Schreiter ER, Shoham S, Razansky D, Boyden ES, Campbell RE TITLE A genetically encoded near-infrared fluorescent calcium ion indicator. JOURNAL Nat Methods. 2019 Feb;16(2):171-174. doi: 10.1038/s41592-018-0294-6. Epub 2019 Jan 21. PUBMED 30664778 REFERENCE 2 (bases 1 to 5804) AUTHORS . TITLE Direct Submission JOURNAL Exported Mar 28, 2023 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5804 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..448 /label=hSyn promoter /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)" misc_feature 2031..2619 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2084..2104) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2502..2513) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(2622..2638) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2623..2639) /label=pBluescriptKS /note="For pBluescript vector" polyA_signal 2668..2789 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2679..2698) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2733..2752 /label=SV40pA-R /note="SV40 polyA, reverse primer" repeat_region 2951..3080 /label=AAV2 ITR primer_bind complement(3146..3163) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3317..3905) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3397..3416) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4076..4936) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4699..4718 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4937..5041) /gene="bla" /label=AmpR promoter rep_origin complement(5067..5522) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5204..5225) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 5416..5435 /label=F1ori-R /note="F1 origin, reverse primer" repeat_region 5594..5723 /label=AAV2 ITR ORIGIN 1 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga 61 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga 121 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt caacaccgcg 181 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc 241 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg 301 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg 361 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg 421 aggagtcgtg tcgtgcctga gagcgcagtc gaattcgggg atccatgctg cagaacgagc 481 ttgctcttaa gttggctgga cttgatatta acaagactgg aggaggttct atgtcggtac 541 cgctgactac ctcagcattc ggccacgcgt ttctggctaa ctgtgaacgt gagcagatcc 601 acctggcggg ctccattcag ccgcacggta tcctgctggc tgttaaagag cctgacaacg 661 tggtgatcca ggcttctatt aacgctgcgg agttcctgaa caccaacttt gttgttggcc 721 gtccgctgcg tgacctgggc ggcgatctgc ctttgcagat cctgccgcac ctgaacggcc 781 cgctgcacct ggctccgatg accctgcgtt gtactgtggg ttctccgccg cgtcgtgtgg 841 actgtaccat tcatcgaccg tctaacggcg gcctgatcgt agaactggaa ccagcaacca 901 aggccactaa cattgcgccg gctctggtcg gtgcgcttca tcgtatcact tcttcatcct 961 ccctgatggg cctgtgtgac gaaaccgcga ctattttccg tgagattact ggcttcgacc 1021 gtgtgatggt aatgcgtctc ggcgcgcttg acgatctgac tgaagagcag atcgcagaga 1081 ttaaagaggc tttctcccta tttgacaagg acggggacgg gacgataaca accaaggagc 1141 tggggacggt gttccggtct ctggggcaga accccacaga agcagagctg caggacatga 1201 tcaatgaagt agatgccgat ggcgacggca cattcgactt ccctgagttc ctgacgatga 1261 tggcaaggaa aatgaatgac tcagacagtg aagaggaaat tagagaagcg ttccgcgtgt 1321 ttgataagga cggcaatggc tacatcggcg cagcagagct tcgccacgtg atgacagacc 1381 ttggtgagaa gttaactgat gaggaggttg atgaaatgat cagggtagca gacaacgatg 1441 gggatggtca ggtaaactac gaagagtttg tacaaatgat gacagcgaag ggtggcggag 1501 gttctgtaga ttcatcacgt cgtaagtgga ataaggcagg tcacgcagtc agagctatag 1561 gtcggctgag ctcgcgtagg catgatttgc tgtccgaatg tcgtcgtgcg gacctggaag 1621 cgttcctggg taaccgctac ccggcgtcta ctattccgca gatcgctcgt cgcctgtacg 1681 aacttaaccg tgttcgcctg ctggtagatg tgaactatac tccggttccg ctacagccgc 1741 gcatcagccc gctgaacggt cgtgatctgg atatgtccct gtcttgcctg cgctctatgt 1801 ccccgatcca ccagaaatac atgcaggaca tgggcgttgg cgcaaccctg gtttgctctc 1861 tgatggtgtc tggtcgtctg tggggtctga tcgtttgcca ccactacgaa ccgcgcttcg 1921 ttccgtccca cattcgcgct gctggcgaag cgctggcgga aacttgtgcg aaccgcatcg 1981 cgacgctgga gagctttgca cagtctcagt ccaaatgaaa gcttatcgat aatcaacctc 2041 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 2101 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 2161 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 2221 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 2281 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 2341 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 2401 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg 2461 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 2521 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 2581 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgac 2641 ccgggcggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa 2701 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg 2761 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc 2821 aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta 2881 aaatcgataa ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc 2941 attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg 3001 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca 3061 gtgagcgagc gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 3121 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 3181 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 3241 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 3301 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 3361 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 3421 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 3481 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 3541 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 3601 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 3661 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 3721 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 3781 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 3841 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 3901 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 3961 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 4021 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 4081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 4141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 4201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 4261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 4321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 4381 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 4441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 4501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 4561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 4621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 4681 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 4741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 4801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 4861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 4921 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 4981 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 5041 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc 5101 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 5161 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 5221 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 5281 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 5341 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 5401 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 5461 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 5521 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 5581 gctattacgc cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg 5641 tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc 5701 caactccatc actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac 5761 gtagccatgc tctaggaaga tctctgcaga gggccctgcg tatg //