LOCUS Exported 533 bp ds-DNA linear SYN 10-JUN-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pCruzHA SIRT1 G261A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 533) AUTHORS Nemoto S, Fergusson MM, Finkel T TITLE SIRT1 functionally interacts with the metabolic regulator and transcriptional coactivator PGC-1{alpha}. JOURNAL J Biol Chem. 2005 Apr 22. 280(16):16456-60. PUBMED 15716268 REFERENCE 2 (bases 1 to 533) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 10, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..533 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 28..47 /label=T7 /note="T7 promoter, forward primer" promoter 28..46 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 69..78 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 84..110 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" regulatory 261..270 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagcttc 61 tcgaggccgc caccatggga tcctaccctt acgacgttcc tgattacgct agcctcgaat 121 tcagtactgc ggccgcggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc 181 agattcgaca gagaggcagt tggaagatgg cggacgaggt ggcgctcgcc cttcaggccg 241 ccggctcccc ttccgcggcg gccgccatgg aggccgcgtc gcagccggcg gacgagccgc 301 tccgcaagag gccccgccga gacgggcctg gcctcgggcg cagcccgggc gagccgagcg 361 cagcagtggc gccggcggcc gcggggtgtg aggcggcgag cgccgcggcc ccggcggcgc 421 tgtggcggga ggcggcaggg gcggcggcga gcgcggagcg ggaggccccg gcgacggccg 481 tggccgggga cggagacaat gggtccggcc tgcggcggga gccgagggcg gcc //