LOCUS Exported 8371 bp ds-DNA circular SYN 24-MAY-2023 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSVL(D2m) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8371) AUTHORS Kuo MY, Chao M, Taylor J TITLE Initiation of replication of the human hepatitis delta virus genome from cloned DNA: role of delta antigen. JOURNAL J Virol. 1989 May . 63(5):1945-50. PUBMED 2649689 REFERENCE 2 (bases 1 to 8371) AUTHORS . TITLE Direct Submission JOURNAL Exported May 24, 2023 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8371 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 1..589 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 490..509 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1276..1295 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" polyA_signal 1460..1594 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1497..1516) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1551..1570 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" promoter complement(4973..4991) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(4974..4991) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(4999..5016) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4999..5015) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5008..5030) /label=M13/pUC Forward /note="In lacZ gene" promoter 6314..6671 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(6337..6357) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 6522..6657 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6584..6603 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(7150..7168) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7236..7340 /gene="bla" /label=AmpR promoter CDS 7341..8201 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7559..7578) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 601 cgcggccttt ttacggttcc tggccttttg ctggccttga agctgtccct gatggtcgtc 661 atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag 721 aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc 781 cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc 841 gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag 901 gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc 961 cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt 1021 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg 1081 acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt 1141 gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc 1201 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc 1261 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc 1321 ggtgatgccg gccacgatgc gtccggcgta gaggatcata atcagccata ccacatttgt 1381 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat 1441 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa 1501 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 1561 caaactcatc aatgtatctt atcatgtctg gatccccggg taccgagctc gaattctcgg 1621 aaagaaggat aaggatggag agggggctcc cccggcgaag agggcccgaa cggaccagat 1681 ggaggtagac tccggacctg ggaagaggcc tctcagggga ggattcaccg acaaggagag 1741 gcaggatcac cgacgaagga aggccctcga gaacaagaag aagcagctat cggcgggagg 1801 caagaacctc agcaaggagg aagaagagga actcaggagg ttgaccgagg aagacgagag 1861 aagggaaaga agagtagccg gcccgccggt tgggggtgtg aaccccctcg aaggtggatc 1921 gaggggagcg cccgggggcg gcttcgtccc caatctgcag ggagtcccgg agtccccctt 1981 ctctcggacc ggggaggggc tggacatcag gggaaaccag ggatttccat aggatatact 2041 cttcccagcc gatccgccct tttctcccca gagttgtcga ccccagtgaa taaagcgggt 2101 ttccactcac aggtttgcgt ctcgcgtcct tctttcctct tcgggtcggc atggcatctc 2161 cacctcctcg cggtccgacc tgggcatccg aaggaggacg cacgtccact cggatggcta 2221 agggagagcc acttttctct cgattctcta tcggaatcta gagagatttg tgggtcccat 2281 tcgccattac cgaggggacg gtcccctcgg aatgttgccc agccggcgcc agcgaggagg 2341 ctgggaccat gccggccatc aggtaagaaa ggatggaacg cggaccctgc agagtggggt 2401 cccgccattc ctgggcgacc cttggggggg gagtcggaat cgagcatcgg gaagggcatc 2461 ccatggctcc actggtcccc ggtgttccca gcaccccctc cggtcacttt cgaagggggt 2521 ccggggtccc gctagatggg gacgataagt cgagttcccc gggataagcc tcactcgtcc 2581 cctctcgggg ggcggaacac ccaccggcta gccccgttgc tttctttgct ttcctcctcg 2641 cttcggtctc cccctactcc tagcatctcc tcctatcgct atggtcttac tcctaccgct 2701 cgaagcgcct cttgttcgct gaaggggtcc tctggaggtg atttctctgc tcatctccga 2761 gtggattcct ccctctgagt gctactcaac ccttcgggcc ggagtgctct ccagatctgg 2821 agattgggcc cctcggatcg ggaggcccgc ccctttttct tccaccttcc tctttcccct 2881 tccagagatt ctccggcgtt gtggggatct cgtccgcttc ctaagacctc tctttgcttc 2941 ttgggagtag tttctccgac gttccaatgc tctttaccgt gacatcccct ctcgggagct 3001 gatcctcccc ccgcgtctcc tcgctcggaa cttggctcag gctcgggctc gggcggcgag 3061 tccagcagtc tcctctttac agaaaagagt aagagtactg aggactgccg cctctagccg 3121 agatgagccg gtccgagtcg aggaagaacc gcggagggag agaagagatc ctcgagcagt 3181 gggtggccgg aagaaagaag ttagaggaac tcgagagaga cctccggaag acaaagaaga 3241 aactcaagaa gatagaggac gaaaatccct ggctggggaa catcaggaat tctcggaaag 3301 aaggataagg atggagaggg ggctcccccg gcgaagaggg cccgaacgga ccagatggag 3361 gtagactccg gacctgggaa gaggcctctc aggggaggat tcaccgacaa ggagaggcag 3421 gatcaccgac gaaggaaggc cctcgagaac aagaagaagc agctatcggc gggaggcaag 3481 aacctcagca aggaggaaga agaggaactc aggaggttga ccgaggaaga cgagagaagg 3541 gaaagaagag tagccggccc gccggttggg ggtgtgaacc ccctcgaagg tggatcgagg 3601 ggagcgcccg ggggcggctt cgtccccaat ctgcagggag tcccggagtc ccccttctct 3661 cggaccgggg aggggctgga catcagggga aaccagggat ttccatagga tatactcttc 3721 ccagccgatc cgcccttttc tccccagagt tgtcgacccc agtgaataaa gcgggtttcc 3781 actcacaggt ttgcgtctcg cgtccttctt tcctcttcgg gtcggcatgg catctccacc 3841 tcctcgcggt ccgacctggg catccgaagg aggacgcacg tccactcgga tggctaaggg 3901 agagccactt ttctctcgat tctctatcgg aatctagaga gatttgtggg tcccattcgc 3961 cattaccgag gggacggtcc cctcggaatg ttgcccagcc ggcgccagcg aggaggctgg 4021 gaccatgccg gccatcaggt aagaaaggat ggaacgcgga ccctgcagag tggggtcccg 4081 ccattcctgg gcgacccttg gggggggagt cggaatcgag catcgggaag ggcatcccat 4141 ggctccactg gtccccggtg ttcccagcac cccctccggt cactttcgaa gggggtccgg 4201 ggtcccgcta gatggggacg ataagtcgag ttccccggga taagcctcac tcgtcccctc 4261 tcggggggcg gaacacccac cggctagccc cgttgctttc tttgctttcc tcctcgcttc 4321 ggtctccccc tactcctagc atctcctcct atcgctatgg tcttactcct accgctcgaa 4381 gcgcctcttg ttcgctgaag gggtcctctg gaggtgattt ctctgctcat ctccgagtgg 4441 attcctccct ctgagtgcta ctcaaccctt cgggccggag tgctctccag atctggagat 4501 tgggcccctc ggatcgggag gcccgcccct ttttcttcca ccttcctctt tccccttcca 4561 gagattctcc ggcgttgtgg ggatctcgtc cgcttcctaa gacctctctt tgcttcttgg 4621 gagtagtttc tccgacgttc caatgctctt taccgtgaca tcccctctcg ggagctgatc 4681 ctccccccgc gtctcctcgc tcggaacttg gctcaggctc gggctcgggc ggcgagtcca 4741 gcagtctcct ctttacagaa aagagtaaga gtactgagga ctgccgcctc tagccgagat 4801 gagccggtcc gagtcgagga agaaccgcgg agggagagaa gagatcctcg agcagtgggt 4861 ggccggaaga aagaagttag aggaactcga gagagacctc cggaagacaa agaagaaact 4921 caagaagata gaggacgaaa atccctggct ggggaacatc aggaattcgt attctatagt 4981 gtcacctaaa tccaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 5041 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagggtct agactcgagg 5101 gatcgatccg gtttagagca gaagtaacac ttccgtacag gcctagaagt aaaggcaaca 5161 tccactgagg agcagttctt tgatttgcac caccaggagc ctcaaatttt tcaataaatt 5221 cacctgactg cacattagga ctttggcttt gagcttccca cctctcagtt acttgctgaa 5281 tactgtctgc ttcatcaata ttatcatagg tgtgcccaaa tgatatttgc aacccttccc 5341 tgttggctac ttgtctcacc attgtaggcc taatgggaga caaagtagag tagtaatctt 5401 gtaaagagtt ataccaatta acaggagcat taattactgt ccaagtagtt tcctctaaaa 5461 accttgccaa actgtccctt aaatatcttt gggttcttct ttcaagctcc tgtgaggtga 5521 gcctaggaat gtcattttgt attacacgcc aaaaagcttg agaaatggca ttaaaaagtg 5581 ttggacccca atgtctgggg tcaagatact gaacactgtg aacaaaggtt tgtactccag 5641 gaaataaaat atcataataa tcatctggcc tatacaaatc tacagccatt cctggttgtt 5701 gatataaacc aacagtagaa actttgtgat cccagtcact aaaaaatcta taccccactt 5761 gagcaacagc gctcacacca gtcacagttt gcagtaaagc tgcaaatcca gctatagcag 5821 caggagcccc agatatcaca gcataggcct gtggagtgag gcctatagca gcaattgcct 5881 cagaggttgt taggccttca acagtagcaa cagatgcaag ttgcacttca attgcagcag 5941 cggcctctcc agcagcaatt tcagctactg aaaatccagt agcagcagca gcttcagaca 6001 cagtagcaat taggtccccc aacagtgtta aagcagcacc catggacctg aaataaaaga 6061 caaaaagact aaacttacca gttaactttc tggtttttca gttaaccttt ctggtttttt 6121 gcgtttcccg tcaacagtat cttccccttc acaaaattgc agcaaaagct ctaaaacaaa 6181 cacaaaaagg cgttgagctg tttttttact ttcagtccat gacctacgaa ccttaacgga 6241 ggcctggcgt gacagccggc gcagcaccat ggcctgaaat aacctctgaa agaggaactt 6301 ggttaggtac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg 6361 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 6421 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 6481 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 6541 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 6601 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 6661 gcttttgcaa aaagctttgc aaagatggat aaagttttaa acagagagga atctttgcag 6721 ctaatggacc ttctaggtct tgaaaggagt gcctggggga atattcctct gatgagaaag 6781 gcatatttaa aaaaatgcaa ggagtttcat cctgataaag gaggagatga agaaaaaatg 6841 aagaaaatga atactctgta caagaaaatg gaagatggag taaaatatgc tcatcaacct 6901 gactttggag gcttctggga tgcaactgag gtatttgctt cttccttaaa tcctggtgtt 6961 gatgcaatgt actgcaaaca atggcctgag tgtgcaaaga aaatgtctgc taactgcata 7021 tgcttgctgt gcttactgag gatgaagcat gaaaatagaa aattatacag gaaagatcca 7081 cttgtgtggg ttgattgcta ctgcttcgat gataagctgt caaacatgag aattcttgaa 7141 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 7201 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 7261 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 7321 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 7381 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 7441 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 7501 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 7561 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 7621 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 7681 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 7741 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 7801 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 7861 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg 7921 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 7981 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 8041 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 8101 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 8161 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 8221 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 8281 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 8341 cagaccccgt agaaaagatc aaaggatctt c //