LOCUS       Exported                6631 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Retroviral expression of canonical human GLIS1.
ACCESSION   .
VERSION     .
KEYWORDS    pMXs-GLIS1 (FLJ33315SAAN)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Maekawa M, Yamaguchi K, Nakamura T, Shibukawa R, Kodanaka I, 
            Ichisaka T, Kawamura Y, Mochizuki H, Goshima N, Yamanaka S
  TITLE     Direct reprogramming of somatic cells is promoted by maternal 
            transcription factor Glis1.
  JOURNAL   Nature. 2011  . 474(7350):225-9.
  PUBMED    21654807
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1100..1516
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1446..1468
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1484..1500
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1526..1900
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1838..1854
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1951..1975
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3857..3881)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             4076..4669
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4735..4752)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4906..5494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4986..5005)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5658..6518)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6281..6300
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6519..6623)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaag cggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggatctcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct tagaggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat atgggcccgg gccagactgt taccactccc ttaagtttga ccttaggtca
     1141 ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg
     1201 ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac
     1261 ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg
     1321 acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc
     1381 ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc
     1441 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac
     1501 tccttctcta ggcgccccca tatggccata tgagatctta tatggggcac ccccgcccct
     1561 tgtaaacttc cctgaccctg acatgacaag agttactaac agcccctctc tccaagctca
     1621 cttacaggct ctctacttag tccagcacga agtctggaga cctctggcgg cagcctacca
     1681 agaacaactg gaccgaccgg tggtacctca cccttaccga gtcggcgaca cagtgtgggt
     1741 ccgccgacac cagactaaga acctagaacc tcgctggaaa ggaccttaca cagtcctgct
     1801 gaccaccccc accgccctca aagtagacgg catcgcagct tggatacacg ccgcccacgt
     1861 gaaggctgcc gaccccgggg gtggaccatc ctctagactg ccggatctag ctagttaatt
     1921 aaggatccca gtgtggtggt acgggaaatc acaagtttgt acaaaaaagc aggcttcgaa
     1981 ggagatagaa ccatggcaga ggcccgcaca tccctgtctg cccactgtcg gggcccgctg
     2041 gccactggcc tgcacccaga cctggacctc ccgggccgaa gcctcgccac ccctgcgcct
     2101 tcctgctacc ttctgggcag cgaacccagc tctggcctgg gcctccagcc cgagacccac
     2161 ctccccgagg gcagcctgaa gcggtgctgt gtcttgggcc taccccccac ctccccagcc
     2221 tcctcctcac cctgtgcctc ctccgacgtc acctccatca tccgctcctc ccagacgtct
     2281 ctggtcacct gtgtaaatgg actccggagc ccccctctga cgggagatct ggggggccct
     2341 tccaagcggg cccggcctgg ccctgcatcg acggacagcc atgagggcag cttgcaactt
     2401 gaagcctgcc ggaaggcgag cttcctgaag caggaacccg cggatgagtt ttcagagctc
     2461 tttgggcctc accagcaggg cctgccgccc ccctatcccc tgtctcagtt gccgcctggc
     2521 ccaagccttg gaggcctggg gctgggcctg gcaggcaggg tggtggccgg gcggcaggcg
     2581 tgccgctggg tggactgctg tgcagcctat gagcagcagg aggagctggt gcggcacatc
     2641 gagaagagcc acatcgacca gcgcaagggc gaggacttca cctgcttctg ggctggctgc
     2701 gtgcgccgct acaagccctt caacgcccgc tacaagctgc tcatccacat gcgagtgcac
     2761 tcgggcgaga agcccaacaa gtgcatgttt gaaggctgca gcaaggcctt ctcacggctg
     2821 gagaacctca agatccacct gaggagccac acgggcgaga agccgtacct gtgccagcac
     2881 ccgggttgcc agaaggcctt cagcaactcc agcgaccgcg ccaagcacca gcgcacccac
     2941 ctagacacga agccgtacgc ctgtcagatc cctggctgct ccaagcgcta cacagacccc
     3001 agctccctcc gcaagcacgt caaggcccat tcagccaaag agcagcaggt gcgtaagaag
     3061 ctgcatgcgg gccctgacac cgaggccgac gtcctgaccg agtgtctggt cctgcagcag
     3121 ctccacacgt ccacacagct ggctgccagc gacggcaagg gtggctgtgg cctgggccag
     3181 gagctgctcc caggtgtgta tcctggctcc atcacccccc ataacggact tgcatcgggc
     3241 ctcctgcccc cagcgcacga cgtaccttcc aggcaccacc cgctggatgc caccaccagt
     3301 tcccaccacc atctgtcccc tctgcccatg gctgagagca cccgggatgg gttggggccc
     3361 ggcctcctct caccaatagt cagccccctg aaggggctgg ggccaccgcc gctgccccca
     3421 tcctctcaga gccattctcc ggggggccag cccttcccca cactccccag caagccgtcc
     3481 tacccaccct tccagagccc tccacccccg cctctgccca gcccacaagg ttaccagggc
     3541 agtttccact ccatccagag ttgcttcccc tatggcgact gctaccggat ggctgaacca
     3601 gcagccggtg gggacggact ggtcggggag acccacggtt tcaaccccct gcggcccaat
     3661 ggctaccaca gcctcagcac gcccttgcct gccacaggct atgaggccct ggctgaggcc
     3721 tcatgcccca cagcgctgcc acagcagcca tctgaagatg tggtgtccag cggccccgag
     3781 gactgtggct tcttccccaa tggagccttt gaccactgcc tgggccacat cccctccatc
     3841 tacacagaca cctaagaccc agctttcttg tacaaagtgg tgatattcca gctgagcgcc
     3901 ggtcgctacc attaccagtt ggtctggtgt caaaaataat aataaccggg caggccatgt
     3961 ctgcccgtat ttcgcgtaag gaaatccatt atgtactatt taaactcgag cggccgccag
     4021 cacagtggtc gacgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     4081 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     4141 aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg
     4201 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     4261 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     4321 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
     4381 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     4441 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     4501 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     4561 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     4621 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattc tgcattaatg
     4681 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     4741 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4801 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4861 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4921 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4981 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5041 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5101 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5161 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5221 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5281 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5341 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5401 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5461 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5521 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5581 aaggatcttc acctagatcc ttttgcggcc ggccgcaaat caatctaaag tatatatgag
     5641 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5701 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5761 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5821 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5881 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5941 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6001 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6061 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6121 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6181 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6241 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     6301 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6361 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6421 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6481 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6541 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6601 aataaacaaa taggggttcc gcgcacattt c
//
