LOCUS Exported 4769 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pJMK001 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4769) AUTHORS Kralj JM, Hochbaum DR, Douglass AD, Cohen AE TITLE Electrical spiking in Escherichia coli probed with a fluorescent voltage-indicating protein. JOURNAL Science. 2011 Jul 15;333(6040):345-8. PUBMED 21764748 REFERENCE 2 (bases 1 to 4769) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4769 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..285 /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind 208..227 /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" RBS 312..334 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 1096..1113 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(1169..1186) /label=pBAD Reverse /note="For vectors with E. coli araBAD promoter, reverse primer" terminator 1339..1425 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1517..1544 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" promoter 1564..1655 /gene="bla" /label=AmpR promoter CDS 1656..2516 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1874..1893) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2687..3275 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3176..3195 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3429..3446 /label=L4440 /note="L4440 vector, forward primer" misc_feature 3461..3601 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(3527..3546) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3687..3709 /label=pGEX 3' /note="pGEX vectors, reverse primer" CDS complement(3865..4743) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" ORIGIN 1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg 301 ctagaaataa ttttgtttaa ctttaagaag gagatataca tacccatggg taaattatta 361 ctgatattag gtagtgttat tgcacttcct acatttgctg caggtggtgg tgaccttgat 421 gctagtgatt acactggtgt ttctttttgg ttagttactg ctgctttatt agcatctact 481 gtatttttct ttgttgaaag agatagagtt tctgcaaaat ggaaaacatc attaactgta 541 tctggtcttg ttactggtat tgctttctgg cattacatgt acatgagagg ggtatggatt 601 gaaactggtg attcgccaac tgtatttaga tacattaatt ggttactaac agttcctcta 661 ttaatatgtg aattctactt aattcttgct gctgcaacta atgttgctgg atcattattt 721 aagaaattac tagttggttc tcttgttatg cttgtgtttg gttacatggg tgaagcagga 781 atcatggctg catggcctgc attcattatt gggtgtttag cttgggtata catgatttat 841 gaattatggg ctggagaagg aaaatctgca tgtaatactg caagtcctgc tgtgcaatca 901 gcttacaaca caatgatgta tattatcatc tttggttggg cgatttatcc tgtaggttat 961 ttcacaggtt acctgatggg tgacggtgga tcagctccta acttaaacct tatctataac 1021 cttgctgact ttgttaacaa gattctattt ggtttaatta tatggaatgt tgctgttaaa 1081 gaatcttcta atgctcacca tcaccatcac cattgagttt aaacggtctc cagcttggct 1141 gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg 1201 gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc 1261 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag 1321 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 1381 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat 1441 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc 1501 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact 1561 ctttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 1621 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 1681 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 1741 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 1801 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 1861 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac 1921 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 1981 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 2041 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 2101 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 2161 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 2221 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 2281 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 2341 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 2401 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 2461 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 2521 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 2581 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 2641 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 2701 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 2761 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 2821 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 2881 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 2941 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 3001 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 3061 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 3121 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 3181 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 3241 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 3301 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 3361 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 3421 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc 3481 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg 3541 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc 3601 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 3661 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 3721 atcaccgaaa cgcgcgaggc agcagatcaa ttcgcgcgcg aaggcgaagc ggcatgcata 3781 atgtgcctgt caaatggacg aagcagggat tctgcaaacc ctatgctact ccgtcaagcc 3841 gtcaattgtc tgattcgtta ccaattatga caacttgacg gctacatcat tcactttttc 3901 ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga 3961 gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt 4021 ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct 4081 aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg 4141 tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc 4201 ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg 4261 cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg 4321 cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg 4381 gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc 4441 gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta 4501 gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg 4561 tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat 4621 tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc 4681 cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc 4741 catacttttc atactcccgc cattcagag //