LOCUS       Exported                8621 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    K44A HA-dynamin 1 pcDNA3.1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8621)
  TITLE     wt HA-dynamin 1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      991..1000
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(4215..4232)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4221..4445
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4491..4919
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4578..4597)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4788..4809
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4928..4948)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4933..5262
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5113..5248
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5175..5194
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5329..6123
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5383..5402)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5993..6012
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6297..6418
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6334..6353)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6388..6407
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6467..6483)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6467..6483)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6480..6502)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6491..6507
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6515..6545)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6560..6581
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6698..6715)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6869..7454)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6949..6968)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7625..8485)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8248..8267
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8486..8590)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc
      961 accacactgg actagtggat ccaaggagcc gccgccatgg agtatgatgt tcctgattat
     1021 gctcatatgg gcaaccgcgg catggaagat ctcatcccgc tggtcaaccg gctgcaagac
     1081 gccttctctg ccatcggcca gaacgcggac ctcgacctgc cgcagatcgc tgtggtgggc
     1141 ggccagagcg ccggcgcgag ctcggtgctc gagaatttcg taggcaggga cttcttgcct
     1201 cgaggatctg gcattgtcac ccgacgtccc ctggtcttgc agctggtcaa tgcaaccaca
     1261 gaatatgccg agttcctgca ctgcaaggga aagaaattca ccgacttcga ggaggtgcgc
     1321 cttgagatcg aggccgagac cgacagggtc accggcacca acaagggcat ctcgccggtg
     1381 cctatcaacc tccgcgtcta ctcgccgcac gtgctgaacc tgaccctggt ggacctgccc
     1441 ggaatgacca aggtcccggt gggggaccaa cctcccgaca tcgagttcca gatccgagac
     1501 atgcttatgc agtttgtcac caaggagaac tgcctcatcc tggccgtgtc ccccgccaac
     1561 tctgacctgg ccaattctga cgccctcaag gtcgccaagg aggtggaccc ccagggccag
     1621 cgcaccatcg gggtcatcac caagctggac ctgatggacg agggcacaga tgcccgtgat
     1681 gtgctggaga acaagctgct ccccctgcgc agaggctaca ttggagtggt gaaccggagc
     1741 cagaaggaca ttgatggcaa gaaggacatt accgccgcct tggctgctga acgaaagttc
     1801 ttcctctccc atccatctta tcgccacttg gctgaccgta tgggcacgcc ctacctgcag
     1861 aaggtcctca atcagcaact gacgaaccac atccgggaca cactgccggg gctgcggaac
     1921 aagctgcaga gccagctact gtccattgag aaggaggtgg aggaatacaa gaacttccgc
     1981 cctgatgacc cagctcgcaa gaccaaggcc ctgctgcaga tggtccagca gtttgccgta
     2041 gactttgaga agcgcattga gggctcagga gatcagatcg acacctacga actgtcaggg
     2101 ggagcccgca ttaaccgaat cttccacgag cgcttccctt tcgagctggt caagatggag
     2161 tttgatgaga aggaactccg aagggagatc agctatgcta tcaagaatat ccatggcatt
     2221 agaacggggc tgtttacccc agacatggcc tttgagacca ttgtgaaaaa gcaggtgaag
     2281 aagatccgag aaccgtgtct caagtgtgtg gacatggtta tctcggagct aatcagcacc
     2341 gttagacagt gcaccaagaa gctccagcag tacccgcggc tacgggagga gatggagcgc
     2401 atcgtgacca cccacatccg ggagcgcgag ggccgcacta aggagcaggt catgcttctc
     2461 atcgatatcg agctggctta catgaacacc aaccatgagg acttcatagg ctttgccaat
     2521 gctcagcaga ggagcaacca gatgaacaag aagaagactt cagggaacca ggatgagatt
     2581 ctggtcatcc gcaagggctg gctgactatc aataatattg gcatcatgaa agggggctcc
     2641 aaggagtact ggtttgtgct gactgctgag aatctgtcct ggtacaagga tgatgaggag
     2701 aaagagaaga aatacatgct gtctgtggac aacctcaagc tgcgggacgt ggagaagggc
     2761 tttatgtcga gcaagcatat ctttgccctc tttaacacgg agcagaggaa tgtctacaag
     2821 gattatcggc agctggagct agcctgtgag acacaggagg aggtggacag ctggaaggcc
     2881 tccttcctga gggctggcgt gtaccctgag cgtgttgggg acaaagagaa agccagcgag
     2941 accgaggaga atggctccga cagcttcatg cattccatgg acccacagct ggaacggcaa
     3001 gtggagacca tccggaatct tgtggactca tacatggcca ttgtcaacaa gaccgtgagg
     3061 gacctcatgc ccaagaccat catgcacctc atgattaaca ataccaagga gttcatcttc
     3121 tcggagctgc tggccaacct gtactcgtgt ggggaccaga acacgctgat ggaggagtcg
     3181 gcggagcagg cacagcggcg cgacgagatg ctgcgcatgt accacgcact gaaggaggcg
     3241 ctcagcatca tcggcaacat caacacgacc accgtcagca cgcccatgcc cccgcccgtg
     3301 gacgactcct ggctgcaggt gcagagcgta ccggccggac gcaggtcgcc cacgtccagc
     3361 cccacgccgc agcgccgagc ccccgccgtg cccccagccc ggcccgggtc gcggggccct
     3421 gctcctgggc ctccgcctgc tgggtccgcc ctgggggggg cgccccccgt gccctccagg
     3481 ccgggggctt cccctgaccc tttcggccct ccccctcagg tgccctcgcg ccccaaccgc
     3541 gccccgcccg gggtccccag ccgatcgggt caggcaagtc catcccgtcc tgagagcccc
     3601 aggcccccct tcgacctcta aacagatccc tcctcttctc ggagacctcc ctttccaagc
     3661 ctgcctggac ggctgttctg tgacttgaca gtggctcccc cagccccaaa gccagccccc
     3721 ttcatctgtg acttaatctg ttgtagtggt gagctgatac attcaggtgt gaccgttggt
     3781 gaaaacttgt gccccttctg tggtatgccc ttgccctgtt ctataaatat ctataaatac
     3841 tcatatatat acacacctac acatggccaa ccgcctcgcc tctagcgctg ggaatcagtc
     3901 actgtgctat ccttgtggag tcttgtggcc caactaccag agaacgctgt cccccgacat
     3961 cccactccaa agtgtgccac ctccagtgag cctccttgtc atgcccggcc tgtggacagc
     4021 cagcccccgc catccctccc accccctacc aagcatgggg gtgctgtgca ggcagccgtg
     4081 tggcctgaca gtttctacca gtcctgctgt ccctcggctg agaataaaac ccatttctgg
     4141 atgatggaaa aaaaaaaaaa aaaaaaaaaa aaaagggaat tcgatatcaa gcttaagttt
     4201 aaaccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4261 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4321 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4381 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4441 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4501 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4561 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4621 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4681 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4741 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4801 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4861 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     4921 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     4981 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5041 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5101 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5161 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     5221 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     5281 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     5341 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     5401 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     5461 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     5521 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     5581 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     5641 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     5701 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     5761 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     5821 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     5881 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     5941 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     6001 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     6061 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     6121 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     6181 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6241 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     6301 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6361 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6421 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6481 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6541 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6601 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6661 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6721 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6781 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6841 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6901 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6961 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7021 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7081 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7141 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7201 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7261 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7321 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7381 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7441 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7501 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7561 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7621 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7681 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7741 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7801 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7861 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7921 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7981 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8041 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8101 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8161 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8221 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8281 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8341 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8401 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8461 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8521 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8581 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
