LOCUS Exported 8747 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS wt HA-dynamin 2 pcDNA3.1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8747) TITLE wt HA-dynamin 1 JOURNAL Unpublished REFERENCE 2 (bases 1 to 8747) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8747 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 986..995 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1745..1756 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3858..3992 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4341..4358) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4347..4571 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4617..5045 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4704..4723) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4914..4935 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5054..5074) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5059..5388 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5239..5374 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5301..5320 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5455..6249 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5509..5528) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 6119..6138 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 6423..6544 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6593..6609) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6593..6609) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6606..6628) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6617..6633 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6641..6671) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6686..6707 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6824..6841) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6995..7580) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7075..7094) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7751..8611) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8374..8393 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8612..8716) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc 961 tgcagcccgg gggatccaag gagccgccgc catggagtat gatgttcctg attatgctca 1021 tatgggcaac cgcggcatgg aagagctcat cccgctggtt aacaagctgc aggacgcctt 1081 cagctccatc ggtcagagct gccacctgga cctgccgcag atcgccgtgg tgggcggcca 1141 gagcgccggc aagagttcgg tgctcgagaa cttcgtgggc cgggacttcc ttccacgagg 1201 atcaggaatt gtcacccgga ggcctctcat tctgcagctc atcttttcca aaacagaata 1261 tgcggagttt ttgcactgca agtccaaaaa gtttacagac tttgatgaag tccggcagga 1321 gatcgaagca gagactgacc gggtcacagg caccaacaaa ggcatctccc cggtgcccat 1381 caaccttcgg gtctattcac cacacgtgtt gaacttgacc ctcatcgacc tcccaggcat 1441 cactaaggtg ccagttgggg accagccacc agacatcgag taccagatca aggacatgat 1501 cctgcagttt atcagccggg agagcagcct cattcttgcc gtcacacctg ccaacatgga 1561 cttggccaac tcggatgccc tcaagctggc caaggaggtg gacccccaag gcttgcggac 1621 cattggtgtc atcaccaagc tagacctgat ggatgaaggc acagatgcca gggatgtcct 1681 ggagaacaag ctcctgccct tgagaagagg ctacatcggc gtggttaacc gcagccagaa 1741 agacatcgag ggcagaaagg acatccgggc agctctggca gctgagagga aattcttcct 1801 ctcccaccca gcctaccggc acatggctga ccgcatgggt accccacact tgcagaaaac 1861 actgaaccag caactgacca accacatccg ggagtcactg ccaaccctgc gcagcaagct 1921 gcagagccaa ctgctgtccc tggagaagga agtggaagag tacaagaatt tccggcctga 1981 cgaccccaca cgcaagacca aagccctgct gcagatggtc cagcagtttg gagtggactt 2041 tgagaagcga atcgaaggct ctggagatca agtcgacacc ctggagctgt ctgggggcgc 2101 ccgcatcaat cgcatcttcc acgagcgctt tccctttgag ctggtgaaga tggagtttga 2161 cgagaaagac ctccgaagag agatcagcta cgccattaag aacatccacg gagtcagaac 2221 tgggctcttc acgccggatt tggcgttcga ggccattgtg aaaaagcagg tggtcaagct 2281 gaaagagccc tgtctgaaat gcgtggacct ggttatccag gagctaatca gtacagttag 2341 gcaatgcacc agcaagctca gttcctaccc ccgccttcga gaggagaccg aacgcattgt 2401 caccacctac atcagggagc gagaagggag aaccaaggac cagatccttc ttctgattga 2461 cattgagcag tcctacatca acacaaacca tgaagacttc attggatttg ccaatgccca 2521 gcagaggagc acgcagctga acaagaagag ggccatacct aaccaggggg agatcttggt 2581 gatccgcagg ggctggttga ccatcaacaa catcagcttg atgaaaggcg gttccaagga 2641 gtactggttc gtgctgactg ctgagtcgtt gtcttggtac aaggatgaag aggaaaaaga 2701 aaagaagtac atgctgccac tagacaacct caaaatacgg gatgtggaga agggcttcat 2761 gtccaacaag catgtgtttg ccatcttcaa cacagagcag aggaacgtct acaaggacct 2821 gcgacagatc gaactggctt gtgactccca ggaagatgtg gacagctgga aggcttcgtt 2881 cctgcgagct ggggtctacc cagagaagga ccaggcagag aacgaggatg gagcacaaga 2941 gaacaccttc tccatggacc cgcagctgga gcggcaggtg gagaccatcc gcaacttggt 3001 ggactcctat gtggccatca tcaacaagtc catccgggac ctcatgccaa agaccatcat 3061 gcacctcatg atcaacaaca caaaggcctt catccaccat gagctgctag cctacctgta 3121 ctcatcagca gaccagagca gcctcatgga ggagtcagct gagcaggctc agcggcggga 3181 cgatatgctg cgcatgtacc atgcactcaa ggaagcgctc aacatcattg gggacatcag 3241 caccagcacc gtgtccacgc ctgtgccccc acctgtcgat gatacttggc tccagaacac 3301 cagcagccac agccccactc cacagcgcag acctgtgtcc agtgtgcacc cgccgggccg 3361 cccacccgca gtgcggggcc ccacaccggg gcctcccctc attcctatgc ctgtgggggc 3421 cacatcctcc ttctcggcac cccccatccc atcccggcct ggaccacaga atgtgtttgc 3481 caacaatgac cccttctctg cgccacctca gataccgtct cggccagcac ggattccccc 3541 tgggatccct ccaggagtgc ccagcagaag agcgcccgct gcgcccagcc ggcccaccat 3601 tatccgccca gccgagccgt ccctgctcga ctaggctgca ggggacagtg ttctgggggg 3661 gtcctcgtgt acctacagtg gtctgggccc tgctactgcc ccccatgctg ggaccaggct 3721 ccccgtgggc aagcctgccc tctcctccct agccctagtg cacatgataa ggagggagag 3781 ggtgggaagg aggcccaaac aaacagaact tgggggcttt gcactttggg gcgaggggga 3841 gctcgtcagt tctagaggat ccagacatga taagatacat tgatgagttt ggacaaacca 3901 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat 3961 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt 4021 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg 4081 gtatggctga ttatgatcct gcaagcctcg tcgtctggcc ggaccacgct atctgtgcaa 4141 ggtccccgga cgcgcgctcc atgagcagag cgcccgccgc cgaggcaaga ctcgggcggc 4201 gccctgcccg tcccaccagg tcaacaggcg gtaaccggcc tcttcatcgg gaatgcgcgc 4261 gaccttcagc atcgccggca tgtcccctgg cggacgggaa gtatcagctc gaccaagctt 4321 aagtttaaac cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 4381 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 4441 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 4501 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 4561 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc 4621 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 4681 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 4741 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 4801 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 4861 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 4921 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 4981 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 5041 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 5101 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 5161 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 5221 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 5281 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta 5341 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga 5401 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt 5461 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat 5521 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 5581 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac 5641 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac 5701 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc 5761 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg 5821 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 5881 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat 5941 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag 6001 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc 6061 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg 6121 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 6181 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag 6241 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat 6301 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc 6361 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc 6421 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 6481 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 6541 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 6601 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6661 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6721 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6781 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6841 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6901 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6961 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 7021 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 7081 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 7141 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 7201 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7261 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7321 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7381 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 7441 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7501 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc 7561 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 7621 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 7681 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 7741 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 7801 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 7861 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 7921 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 7981 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 8041 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 8101 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 8161 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 8221 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 8281 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 8341 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 8401 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 8461 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 8521 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 8581 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 8641 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 8701 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc //