LOCUS       Exported                8747 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    K44A HA-dynamin 2 pcDNA3.1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8747)
  TITLE     wt HA-dynamin 1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8747
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      986..995
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1745..1756
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3858..3992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4341..4358)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4347..4571
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4617..5045
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4704..4723)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4914..4935
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5054..5074)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5059..5388
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5239..5374
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5301..5320
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5455..6249
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5509..5528)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6119..6138
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6423..6544
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6593..6609)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6593..6609)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6606..6628)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6617..6633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6641..6671)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6686..6707
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6824..6841)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6995..7580)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7075..7094)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7751..8611)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8374..8393
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8612..8716)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc
      961 tgcagcccgg gggatccaag gagccgccgc catggagtat gatgttcctg attatgctca
     1021 tatgggcaac cgcggcatgg aagagctcat cccgctggtt aacaagctgc aggacgcctt
     1081 cagctccatc ggtcagagct gccacctgga cctgccgcag atcgccgtgg tgggcggcca
     1141 gagcgccggc gcgagttcgg tgctcgagaa cttcgtgggc cgggacttcc ttccacgagg
     1201 atcaggaatt gtcacccgga ggcctctcat tctgcagctc atcttttcca aaacagaata
     1261 tgcggagttt ttgcactgca agtccaaaaa gtttacagac tttgatgaag tccggcagga
     1321 gatcgaagca gagactgacc gggtcacagg caccaacaaa ggcatctccc cggtgcccat
     1381 caaccttcgg gtctattcac cacacgtgtt gaacttgacc ctcatcgacc tcccaggcat
     1441 cactaaggtg ccagttgggg accagccacc agacatcgag taccagatca aggacatgat
     1501 cctgcagttt atcagccggg agagcagcct cattcttgcc gtcacacctg ccaacatgga
     1561 cttggccaac tcggatgccc tcaagctggc caaggaggtg gacccccaag gcttgcggac
     1621 cattggtgtc atcaccaagc tagacctgat ggatgaaggc acagatgcca gggatgtcct
     1681 ggagaacaag ctcctgccct tgagaagagg ctacatcggc gtggttaacc gcagccagaa
     1741 agacatcgag ggcagaaagg acatccgggc agctctggca gctgagagga aattcttcct
     1801 ctcccaccca gcctaccggc acatggctga ccgcatgggt accccacact tgcagaaaac
     1861 actgaaccag caactgacca accacatccg ggagtcactg ccaaccctgc gcagcaagct
     1921 gcagagccaa ctgctgtccc tggagaagga agtggaagag tacaagaatt tccggcctga
     1981 cgaccccaca cgcaagacca aagccctgct gcagatggtc cagcagtttg gagtggactt
     2041 tgagaagcga atcgaaggct ctggagatca agtcgacacc ctggagctgt ctgggggcgc
     2101 ccgcatcaat cgcatcttcc acgagcgctt tccctttgag ctggtgaaga tggagtttga
     2161 cgagaaagac ctccgaagag agatcagcta cgccattaag aacatccacg gagtcagaac
     2221 tgggctcttc acgccggatt tggcgttcga ggccattgtg aaaaagcagg tggtcaagct
     2281 gaaagagccc tgtctgaaat gcgtggacct ggttatccag gagctaatca gtacagttag
     2341 gcaatgcacc agcaagctca gttcctaccc ccgccttcga gaggagaccg aacgcattgt
     2401 caccacctac atcagggagc gagaagggag aaccaaggac cagatccttc ttctgattga
     2461 cattgagcag tcctacatca acacaaacca tgaagacttc attggatttg ccaatgccca
     2521 gcagaggagc acgcagctga acaagaagag ggccatacct aaccaggggg agatcttggt
     2581 gatccgcagg ggctggttga ccatcaacaa catcagcttg atgaaaggcg gttccaagga
     2641 gtactggttc gtgctgactg ctgagtcgtt gtcttggtac aaggatgaag aggaaaaaga
     2701 aaagaagtac atgctgccac tagacaacct caaaatacgg gatgtggaga agggcttcat
     2761 gtccaacaag catgtgtttg ccatcttcaa cacagagcag aggaacgtct acaaggacct
     2821 gcgacagatc gaactggctt gtgactccca ggaagatgtg gacagctgga aggcttcgtt
     2881 cctgcgagct ggggtctacc cagagaagga ccaggcagag aacgaggatg gagcacaaga
     2941 gaacaccttc tccatggacc cgcagctgga gcggcaggtg gagaccatcc gcaacttggt
     3001 ggactcctat gtggccatca tcaacaagtc catccgggac ctcatgccaa agaccatcat
     3061 gcacctcatg atcaacaaca caaaggcctt catccaccat gagctgctag cctacctgta
     3121 ctcatcagca gaccagagca gcctcatgga ggagtcagct gagcaggctc agcggcggga
     3181 cgatatgctg cgcatgtacc atgcactcaa ggaagcgctc aacatcattg gggacatcag
     3241 caccagcacc gtgtccacgc ctgtgccccc acctgtcgat gatacttggc tccagaacac
     3301 cagcagccac agccccactc cacagcgcag acctgtgtcc agtgtgcacc cgccgggccg
     3361 cccacccgca gtgcggggcc ccacaccggg gcctcccctc attcctatgc ctgtgggggc
     3421 cacatcctcc ttctcggcac cccccatccc atcccggcct ggaccacaga atgtgtttgc
     3481 caacaatgac cccttctctg cgccacctca gataccgtct cggccagcac ggattccccc
     3541 tgggatccct ccaggagtgc ccagcagaag agcgcccgct gcgcccagcc ggcccaccat
     3601 tatccgccca gccgagccgt ccctgctcga ctaggctgca ggggacagtg ttctgggggg
     3661 gtcctcgtgt acctacagtg gtctgggccc tgctactgcc ccccatgctg ggaccaggct
     3721 ccccgtgggc aagcctgccc tctcctccct agccctagtg cacatgataa ggagggagag
     3781 ggtgggaagg aggcccaaac aaacagaact tgggggcttt gcactttggg gcgaggggga
     3841 gctcgtcagt tctagaggat ccagacatga taagatacat tgatgagttt ggacaaacca
     3901 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     3961 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     4021 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     4081 gtatggctga ttatgatcct gcaagcctcg tcgtctggcc ggaccacgct atctgtgcaa
     4141 ggtccccgga cgcgcgctcc atgagcagag cgcccgccgc cgaggcaaga ctcgggcggc
     4201 gccctgcccg tcccaccagg tcaacaggcg gtaaccggcc tcttcatcgg gaatgcgcgc
     4261 gaccttcagc atcgccggca tgtcccctgg cggacgggaa gtatcagctc gaccaagctt
     4321 aagtttaaac cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     4381 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     4441 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     4501 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     4561 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     4621 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4681 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4741 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4801 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4861 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4921 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4981 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5041 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5101 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     5161 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5221 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5281 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     5341 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     5401 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     5461 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     5521 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     5581 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     5641 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     5701 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     5761 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     5821 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     5881 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     5941 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     6001 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     6061 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     6121 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     6181 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     6241 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     6301 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     6361 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     6421 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6481 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6541 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     6601 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6661 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6721 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6781 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6841 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6901 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6961 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7021 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7081 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7141 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7201 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7261 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7321 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7381 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7441 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7501 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc
     7561 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7621 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7681 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7741 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7801 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7861 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7921 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7981 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8041 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8101 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8161 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8221 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8281 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8341 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8401 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8461 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8521 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8581 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8641 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8701 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
