LOCUS Exported 3576 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pMYO127TAG-SepT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3576) AUTHORS Park HS, Hohn MJ, Umehara T, Guo LT, Osborne EM, Benner J, Noren CJ, Rinehart J, Soll D TITLE Expanding the genetic code of Escherichia coli with phosphoserine. JOURNAL Science. 2011 Aug 26;333(6046):1151-4. PUBMED 21868676 REFERENCE 2 (bases 1 to 3576) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3576 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(277..294) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(277..293) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(286..308) /label=M13/pUC Forward /note="In lacZ gene" promoter complement(513..541) /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" rep_origin 653..1198 /direction=RIGHT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. " primer_bind 1099..1118 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1315..1332 /label=L4440 /note="L4440 vector, forward primer" promoter 1724..1826 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 1827..2486 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1893..1912) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 3210..3227 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" terminator 3258..3344 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 3436..3463 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" ORIGIN 1 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgccgcttct ttgagcgaac 61 gatcaaaaat aagtggcgcc ccatcaaaaa aatattctca acataaaaaa ctttgtgtaa 121 tacttgtaac gctgaattcg ccggggtagt ctaggggtta ggcagcggac tctagatccg 181 ccttacgtgg gttcaaatcc cacccccggc tccaggatcc tctagagtcg acctgcagat 241 ccttagcgaa agctaaggat tttttttaag cttggcactg gccgtcgttt tacaacgtcg 301 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc 361 cagatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag 421 ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact 481 gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca 541 aacatgagaa ttacaactta tatcgtatgg ggctgacttc aggtgctaca tttgaagaga 601 taaattgcac tgaaatctag aaatatttta tctgattaat aagatgatct tcttgagatc 661 gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt 721 tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt ggaggagcgc 781 agccaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc aagactaact 841 cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg 901 gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg gggttcgtgc 961 atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga 1021 caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg 1081 cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 1141 ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa 1201 cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa 1261 tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag 1321 tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg caccggtgca 1381 gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg ccaacatagt 1441 aagccagtat acactccgct agcgctgatg tccggcggtg cttttgccgt tacgcaccac 1501 cccgtcagta gctgaacagg agggacagct gatagaaaca gaagccactg gagcacctca 1561 aaaacaccat catacactaa atcagtaagt tggcagcatc acccgacgca ctttgcgccg 1621 aataaatacc tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg 1681 ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag 1741 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga 1801 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg 1861 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta 1921 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata 1981 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg 2041 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt 2101 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg 2161 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg 2221 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga 2281 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca 2341 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc 2401 atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact 2461 gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc 2521 ctggttgcta cgcctgaata agtgataata agcggatgaa tggcagaaat tcgaaagcaa 2581 attcgacccg ggcggccgca acccagcgtt cgatgcttct ttgagcgaac gatcaaaaat 2641 aagtgccttc ccatcaaaaa aatattctca acataaaaaa ctttgtgtaa tacttgtaac 2701 gctatatgga gattaactca atctagaggg tattacatat ggttctgtct gaaggtgaat 2761 ggcagctggt tctgcacgtt tgggctaaag ttgaagctga cgttgctggt cacggtcagg 2821 acatcctgat ccgtctgttc aaatctcacc cggaaaccct ggaaaaattc gaccgtttca 2881 aacacctgaa aaccgaagct gaaatgaagg cttctgaaga cctgaaaaaa cacggtgtta 2941 ccgttctgac cgctctgggt gctatcctga agaaaaaggg tcaccacgaa gctgaactga 3001 aaccgctggc tcagtctcac gctaccaaac acaaaatccc gatcaaatac ctggagttca 3061 tctctgaagc tatcatccac gttctgcact ctcgtcatcc gggtaacttc ggtgcttagg 3121 ctcagggtgc tatgaacaaa gctctggaac tgttccgtaa agacatcgct gctaaataca 3181 aagaactggg ttaccagggt ggttctggtc atcaccatca ccatcactaa ggcgcgccgt 3241 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 3301 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt 3361 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca 3421 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc 3481 ttttgagatc tatgccccgc gcccaccgga aggagctacc ggcagcggtg cggactgttg 3541 taactcagaa taagaaatga ggccgctcat ggcgtt //