LOCUS Exported 3756 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Wnt10B Non-STOP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3756) AUTHORS Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, Waterman ML TITLE A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. JOURNAL Differentiation. 2012 Sep;84(2):203-13. doi: 10.1016/j.diff.2012.06.004. Epub 2012 Jul 9. PUBMED 22784633 REFERENCE 2 (bases 1 to 3756) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3756 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1881..1980) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1997..2016) /label=T7 /note="T7 promoter, forward primer" promoter complement(1998..2016) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2021..2037) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2021..2037) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2139..2158) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2150..2959 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2217..2236) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3106..3694 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3595..3614 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc gcggccgccc ccttcaccat gctggaggag ccccggccgc ggcctccgcc 721 ctcgggcctc gcgggtctcc tgttcctggc gttgtgcagt cgggctctaa gcaatgagat 781 tctgggcctg aagttgcctg gcgagccgcc gctgacggcc aacaccgtgt gcttgacgct 841 gtccggcctg agcaagcggc agctaggcct gtgcctgcgc aaccccgacg tgacggcgtc 901 cgcgcttcag ggtctgcaca tcgcggtcca cgagtgtcag caccagctgc gcgaccagcg 961 ctggaactgc tccgcgcttg agggcggcgg ccgcctgccg caccacagcg ccatcctcaa 1021 gcgcggtttc cgagaaagtg ctttttcctt ctccatgctg gctgctgggg tcatgcacgc 1081 agtagccacg gcctgcagcc tgggcaagct ggtgagctgt ggctgtggct ggaagggcag 1141 tggtgagcag gatcggctga gggccaaact gctgcagctg caggcactgt cccgaggcaa 1201 gagtttcccc cactctctgc ccagccctgg ccctggctca agccccagcc ctggccccca 1261 ggacacatgg gaatggggtg gctgtaacca tgacatggac tttggagaga agttctctcg 1321 ggatttcttg gattccaggg aagctccccg ggacatccag gcacgaatgc gaatccacaa 1381 caacagggtg gggcgccagg tggtaactga aaacctgaag cggaaatgca agtgtcatgg 1441 cacatcaggc agctgccagt tcaagacatg ctggagggcg gccccagagt tccgggcagt 1501 gggggcggcg ttgagggagc ggctgggccg ggccatcttc attgataccc acaaccgcaa 1561 ttctggagcc ttccagcccc gtctgcgtcc ccgtcgcctc tcaggagagc tggtctactt 1621 tgagaagtct cctgacttct gtgagcgaga ccccactatg ggctccccag ggacaagggg 1681 ccgggcctgc aacaagacca gccgcctgtt ggatggctgt ggcagcctgt gctgtggccg 1741 tgggcacaac gtgctccggc agacacgagt tgagcgctgc cattgccgct tccactggtg 1801 ctgctatgtg ctgtgtgatg agtgcaaggt tacagagtgg gtgaatgtgt gtaagtgcat 1861 tctgaagggt gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca 1921 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg 1981 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca 2041 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc 2101 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat 2161 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta 2221 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa 2281 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac 2341 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca 2401 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc 2461 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt 2521 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt 2581 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt 2641 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt 2701 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt 2761 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata 2821 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg 2881 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat 2941 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac 3001 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc 3061 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 3121 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 3181 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 3241 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 3301 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 3361 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 3421 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 3481 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag 3541 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 3601 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 3661 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 3721 gttcctggcc ttttgctggc cttttgctca catgtt //