LOCUS Exported 7517 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCFJ350 - MCS(ttTi5605, II) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7517) AUTHORS Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM TITLE Improved Mos1-mediated transgenesis in C. elegans. JOURNAL Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865. PUBMED 22290181 REFERENCE 2 (bases 1 to 7517) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7517 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(21..40) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 834..1422 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1323..1342 /label=pBR322ori-F /note="pBR322 origin, forward primer" protein_bind 1559..1579 /gene="mutant version of attB" /label=attB3 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" protein_bind 3007..3031 /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind 3038..3054 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 3038..3054 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3250..3267) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3250..3266) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" CDS 5207..5218 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" protein_bind complement(5364..5388) /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6726..6746) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" primer_bind complement(6950..6969) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7069..7091 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(7129..7147) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7215..7319 /gene="bla" /label=AmpR promoter CDS 7320..663 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" ORIGIN 1 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 61 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 961 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 1021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 1081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 1141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 1201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 1261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 1321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 1381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 1441 tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 1501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccat cgaattatca 1561 actatgtata ataaagttgt gtttttgaac acggcgatat gtatctgtag atcatcccca 1621 attttcaaca atgaatctaa atactgaact ttttgtgtaa tctttagtgc agaaaaatga 1681 ccaaaatcag cgattttccg attataccca aaatatctca gctcaatgag cacctataaa 1741 aatgtaaaaa taaagaaagt agtcgaacca tattttgtaa aggctaaaaa actgaataaa 1801 tttttatcca tccagcgcgg cgttacaatc cgtggcccat gtcacgcacg gctttcaaag 1861 aaaaaaaaac agtctgtttg cgcgacatcg atctggcaaa aatcctaaaa acgactatgc 1921 gtggctcact ttatattaat atgaaaaata caaacttggt ggtgcaggtg ctgcagaggc 1981 catcgatgaa gaagtggagg ccttgttgtc gatctacaaa attgtatggt tgtttgagat 2041 tttccttgaa acgctctcaa tcttggatat gatataaatt ctggataaga tatgatggat 2101 atgatctatg tttgatagag tgcatttgaa aggataacgg ccctgcagac atttttgaaa 2161 aaataacaaa catttaattt tctcactcgt ttaggctatt cccctatttt tgatattcct 2221 tcgcacatat gaaaactact ttttttcgaa actgttaact ccagaattta taaatctata 2281 gcccttactt gattatttat tatcatggtt actcacctca tgtctctttc tcttttcaag 2341 aagtcttcgt tcatatctgt tgtctctttc ctccttttga atctctgctt tgctcttcgc 2401 cattgtttcc tgaaaataat gtaacttgaa ttgtgtaata tactttttta atttgaattt 2461 ggcttgtaac gcggaatcac tacgtgcggg atcatttctt actagaaaac ccagaaaatg 2521 ccatatttca cttatctcgg ggtcatttct aattagaaaa gctacaaaaa ccatttctaa 2581 ttacggtgac tttagaactg gtcgaaatag ctaattagaa atgggttttt gctaattaga 2641 ggtgactcta tgttatctga gtaagcctcg tttgtatgta aactcacagt actaataaat 2701 gcaagacacc cgggtttgtc tagatatgaa ataattgaaa tatcaattct gacagacaat 2761 aatggtaatc ttgataagga gttccacgcc caggagaaca cgttagtttt cttgtttttg 2821 attgcgtgcg ttattttgga gaaaaactcg attttttaca aaataatttt ttgaaaggaa 2881 cactgttcaa taagttttgt cttttttctc agttgtgata cggtttttta ttcttttttg 2941 tagttataca gaagaccgtt acgaaacaga ctgatatcga aacaaacact gatcttactt 3001 gcacttacca ctttgtacaa gaaagctggg tcacgggcag gaaacagcta tgaccatgat 3061 tacgccaagc tacgtaatac gactcactag tgggcagatc ttcgaatgca tcgcgcgcac 3121 cgtacgtctc gaggaattcc tgcaggatat ctggatccac gaagcttccc atggtgacgt 3181 caccggttct agatacctag gtgagctctg gtaccctcta gtcaaggcct taagtgagtc 3241 gtattacgga ctggccgtcg ttttacaccc gtgcagttga aattgaaaat gagttaaagt 3301 tggagttttt attgaaaaca gatttccgtg tgattagtgt ttttagcgag tgtgacagga 3361 cagcgaaaaa atatagaaac aaggggggaa ctgaaaagct taggaatgca ttgaacatga 3421 gaaggggaag gggaaggaac aaactagaca ggaattattg gaatttaatc acatttggag 3481 ttttttttct attcgacaga ataattatcc agaacatttt tgtattaaat atttatgcat 3541 catatgagta gtcggctttg ttgtgcatga cgagtttgtt atcgacgaaa tagaagctgt 3601 cagaacgagt ctcgtttgga ttgttgatca tgtcgtccac tgaaaaagag attagtcttt 3661 tgaattgtac tttttagaat aatgactcac tgagttgttg agagagttga gggaactcat 3721 agatatgttc acagttgttt cgtgaattcg gaatacagaa tccgaattca aagtcaaaac 3781 acttcagaag gcgatctttg aagaagtgac gttcgatcat tcggaaatga tggattggga 3841 tgtctcctac tttgaattcc acagttgctc cgaccgtttt gagtttcaaa aagtttggag 3901 caaatctata acgcacgtat cttgccgact cctgtggcga ctcatcattc tcctgatcgt 3961 tctccggttt ggcgatctcg aaaagcactt gctcagtgtc cagatcacgg atttggaact 4021 tggtgaactc gatgttatag atgttcgcag atggggagca taagaatcct aaatttatgt 4081 tttaaactga aatccaaagg gagcaagata ccttgagtga ttcccggaag tgctaaaacg 4141 tcgttcggag tgatttgagc tttcttcgca agctccgatt ccgttgtgat tccttgttcg 4201 gtgcttggtg gtggccgtgg catctggaaa tatggaaaag ttcaacaaaa agaaaagaga 4261 aaagaatgaa atcggatatc aagagttagt tgagcggttt ctctagtttt ctgagtctca 4321 cctgcgacgg gaaggtcgcc gagccgggtg gaatcgatcg ttgttgctcg gctttcatat 4381 cggtttggtt ggaagcggct gaaaacggaa agaagtggaa gaaggaaaag agtgtggtgt 4441 gacaggaaaa tggtaattag agggtgccaa ataaccagct atattttgtt tttttttgaa 4501 aacattttta aaaagaaaaa tacgataatg atatcagatg gatttccgga aaactggtat 4561 gaaaaatttc aacctttttg agtacatgta atcaaaatac actttgtaaa ttatcatttt 4621 tattgaaact ccaccatttt tctatttata acgctaataa tttgaaaaag aaacctattg 4681 cgaaccgcgg ggtgaatccc aaaaacgaat gcgttttggt ggagtgattg attcgaatcg 4741 aagaagaaaa agaagaagac gtggaataga gagctcactc ttaaccgagc agcacacacc 4801 gacagaaaaa aaaatgaaat gaatgagggt cttcttcttc ttcttcttcg aatgattgac 4861 agaaatggga aaaagaggaa gattgagaag ggaaaaagga aggagaaaag aagcagaaga 4921 agacgtcaga gaggagagga acgagcggaa aagcagcggg cgcaagtcat agaagtagca 4981 gagctgggga gaagaagaca ctatccaaga aaggaatgac gagagagtat gcaaaggggt 5041 atagggtgca gacagaatag gaacagaata acagatgatg agccaagaag agttgaaaag 5101 ggcgatgaat ttgtcatgta acttaatttg ggtcaatttg agcatgatga attgaaatca 5161 tcccttgttg ggagttaata accggtttgt tatcagaaac cctgtaatag aagggcgccc 5221 taactttgag ccaattcatc ccggtttctg tcaaatatat caaaaagtgg tcaactgaca 5281 aattgttttt gatattataa taaacatttt atccgttaac aattttcgaa tactttttac 5341 aaggacttgg ataaattggc tcaagcctgc ttttttgtac aaacttgacg aagtgagttt 5401 gctaccatca tggaatcagg aggacatttc gacaatgtca tataatgttt gaaaaatgat 5461 tttaacaagg gtctcattcg agataagcaa tgctctgagc aagtggctcg tcgtcttcaa 5521 gaatctcttg ttgatattca tttggatcct gttttgcacg aagcatgtgc catggatatt 5581 cagagttttt gcaaaaaagt tccgccaggt caaacaatct ttttaatttt tatatgatac 5641 ttcgggtgta tttttcttct ttttcctgtt cattttcgct tttttctcac ttttttgtaa 5701 caaaatctct cacttttctg taagtcttaa caaaagttca attggctcga tcaagtaaaa 5761 tttcggccgt atgaacgttt tcaaaggttt tcattttttt ttaagttcat tcacaatatc 5821 atgaatctct cgtcctttgg tttccggtaa gtagcgaaat aatataagca tacttataaa 5881 tgaaggaatt gaatatagaa taaggaaagc ataactacca ataacggaat atagaggtaa 5941 tactgtaaat gtactgataa ccaccattac agtattcatt gaatatgcca cagattgagc 6001 aatactacga tgtttttgag gtaccagttc agaggatata aaccacgaaa ttggaccaac 6061 tccagttctg aaatttcatt attcgattaa atcaaaatat atttgaaaaa cctcacccat 6121 aagtataacc gtagatgatc aatgcaacta ggcatccgta tttcatcggg tcaatcagaa 6181 tacgtatttt tgcgaagaat acgaatgcaa ttagagctaa attattgaga aatgtgaaga 6241 gaaggagaag cttgcgtcga ccaactctgt aaacttattc atacaatttt gtcttcaaat 6301 tttgaacgtt taaaactatc ttattcaatg agttagcggg tttttatagt gagttagttc 6361 acgattatct aataaaacta tgtccaaaca tcctaactgc gaccgtattc tcactaactc 6421 gtcttaaaaa cgttgagatt attttaaacc cttcttcagt acaactacag tactgatgca 6481 caaatacaat aataataata cattcagaca tactctgaac aatgtaactt gtttttgtta 6541 tactgtggta acatttattg aaatgaaata taaccttact tttcaatcac aattccacca 6601 gtaattgttc cagaaacata agctaaaccc attgcagttg tactccattc agcaatttct 6661 gactcaacat ttgcattttc tagaaagaaa gtagaggata aaagaagagc ccataatccg 6721 acaagcaact tttctataca aagttgatag cttggcggat ccctggcgtt acccaactta 6781 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg 6841 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc 6901 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct 6961 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac 7021 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 7081 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac 7141 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt 7201 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 7261 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 7321 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 7381 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 7441 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 7501 aagaacgttt tccaatg //