LOCUS       Exported                3853 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Negative selection marker for use with the Mos-1 mediated system of 
            creating targeted transgene insertions and targeted deletions in C. 
            elegans.
ACCESSION   .
VERSION     .
KEYWORDS    pMA122 - peel-1 negative selection
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(465..482)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    500..524
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1053..1077)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      1082..1405
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(1409..1429)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1437..1454)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1437..1453)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1446..1468)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1662..1681)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1781..1803
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1841..1859)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1927..2031
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2032..2892
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2250..2269)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3063..3651
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3552..3571
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3778..3800
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3797..3813
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3797..3813
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    3833..3853
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ctacaggtcg actctagagg atcaccaaaa acggaacgtt gagctggacg gaaatagtgg
       61 taaagtgaca tgattatagt ttgaagattt ctaatttcac aattagagca aatgttgttc
      121 ggtatttatt ttcaacggta tttatactat tttccacctt tttctagaac attcgagctg
      181 cttgttgcaa aaggagggcg actcacattc ggtacatgga aaagtagtgt acacaataaa
      241 gagacccaga tacattttcc gtctgcgtct ctttgcaccc accgggagta ttttcaaacg
      301 aatgcatcta ggaccttcta gaacattctg taaggctgca gaatgcgggt atataaggaa
      361 agcgggctca gaggaagcca acacgctttg ttctagtgca tctaaaaaac ttcgaaaatc
      421 ctcatcggat cctatcgatt cgcggccgct gtacacccgg gtgccctata gtgagtcgta
      481 ttggtaccgg tagaaaaaat caagtttgta caaaaaagca ggcttcatgc gctttgattt
      541 ccaaaactta aaattttcga tgattttcat atttttgtgg aacatcatag tcggattttt
      601 acttgctctt gtaaagattt tcaaaatcta catgtatctt gatctgcctg aaaatgtatg
      661 ggtaaatcgg tttgcgcatg ttattgctct tattggatca attgcttcat tgtggttaat
      721 tttttttagc ccatttgaaa ttaagcaagg aagattttct gtctcgtcaa aaactatcct
      781 gcttcttgtt tgcaagggtt tgattggctt gatatttctt gcacagattt tcgcccatgt
      841 cctttacatt gacccatgcc agaatatgct cttatcattg aactctgcac tgttctcctt
      901 gctgctgaat tattatgagt ctcgtggtgc gatccatccg ttgatggaat tagttcaaac
      961 agctggcctg agaccaactt tgtgcgctgc tgtacgattt aattgtctca actcaactgc
     1021 tcatattgat ccaagtgttg aaaatccatg agacccagct ttcttgtaca aagtgggata
     1081 aatgcaaaat cctttcaagc attcccttct tctctatcac tcttctttct ttttgtcaaa
     1141 aaattctctc gctaatttat ttgctttttt aatgttatta ttttatgact ttttatagtc
     1201 actgaaaagt ttgcatctga gtgaagtgaa tgctatcaaa atgtgattct gtctgatgta
     1261 ctttcacaat ctctcttcaa ttccattttg aagtgcttta aacccgaaag gttgagaaaa
     1321 atgcgagcgc tcaaatattt gtattgtgtt cgttgagtga cccaacaaaa agaggaaact
     1381 ttattgtgcc gccaagaaaa aagtctcaca actttattat acatagttga taattcactg
     1441 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     1501 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     1561 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     1621 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     1681 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     1741 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1801 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     1861 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     1921 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1981 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     2041 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2101 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2161 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     2221 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     2281 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2341 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2401 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2461 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2521 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     2581 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2641 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2701 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2761 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2821 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2881 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2941 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3001 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3061 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3121 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3181 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     3241 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3301 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3361 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3421 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3481 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3541 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3601 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3661 aacgcggcct ttttacggtt cctggtcttt tgctggcctt ttgctcacat gttctttcct
     3721 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccagc
     3781 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct atcaactttg
     3841 tatagaaaag ttg
//
