LOCUS       Exported               15574 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Ai39
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15574)
  AUTHORS   Madisen L, Mao T, Koch H, Zhuo JM, Berenyi A, Fujisawa S, Hsu YW, 
            Garcia AJ 3rd, Gu X, Zanella S, Kidney J, Gu H, Mao Y, Hooks BM, 
            Boyden ES, Buzsaki G, Ramirez JM, Jones AR, Svoboda K, Han X, Turner
            EE, Zeng H
  TITLE     A toolbox of Cre-dependent optogenetic transgenic mice for 
            light-induced activation and silencing.
  JOURNAL   Nat Neurosci. 2012 Mar 25;15(5):793-802. doi: 10.1038/nn.3078.
  PUBMED    22446880
REFERENCE   2  (bases 1 to 15574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15574
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    3..1087
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     enhancer        1181..1484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1486..1763
                     /label=chicken beta-actin promoter
     intron          1765..2782
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2706..2728
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2790..2809
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2858..2891
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(2915..2948)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    3116..3250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3370..3504
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3624..3758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3787..3820)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             4840..5553
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4882..4903)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5143..5162)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5490..5511
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5636..6224
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5689..5709)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6107..6118)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6227..6243)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6228..6244)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6239..6256)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6245..6452
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6460..6493
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        6515..7014
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6550..6573)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6947..6966
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     protein_bind    7034..7067
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      7068..7077
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7074..7877
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7137..7156)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7747..7766
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7887..8348
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     primer_bind     complement(12726..12746)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12726..12744)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12765..12781)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12765..12781)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12778..12800)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12789..12805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12813..12843)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12858..12879
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12996..13013)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13167..13755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13247..13266)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13926..14786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14549..14568
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14787..14891)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(14917..15372)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15054..15075)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15266..15285
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15499..15521
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15513..15530
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15514..15530
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15540..15559
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15540..15558
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggascgctt ccggccgacg tctcgtcgct gattggctty ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgn acgtgcgggc gcggtcggcc tctggcgggg cgggggaggg
      421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttgcccc ctcttcccct cgtgatctgc aactccagtc
     1081 tttctagtta attaaccgtt taaacaattc tgcaggaatc tagttattaa tagtaatcaa
     1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     1501 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     1561 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
     1621 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
     1681 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     1741 aaaagcgaag cgcgcggcgg gcggggagtc gctgcgacgc tgccttcgcc ccgtgccccg
     1801 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     1861 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
     1921 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg
     1981 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc
     2041 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag
     2101 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg
     2161 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc
     2221 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc
     2281 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg
     2341 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg
     2401 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
     2461 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc
     2521 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
     2581 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
     2641 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
     2701 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     2761 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     2821 ttttggcaaa gaattgattt gataccgcgg gccctaagaa gttcctattc tctagaaagt
     2881 ataggaactt cgtcgacatt taaatcattt aaatataact tcgtataatg tatgctatac
     2941 gaagttattc gcgatgaata aatgaaagct tgcagatctg cgactctaga ggatctgcga
     3001 ctctagagga tcataatcag ccntaccaca ttttgtagag gttttactng ctttaaaaaa
     3061 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     3121 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     3241 tgtctggatc tgcgactcta gaggatcata atcagccata ccacatttgt agaggtttta
     3301 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     3361 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3421 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3481 aatgtatctt atcatgtctg gatctgcgac tctagaggat cataatcagc cataccacat
     3541 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     3601 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     3661 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3721 tgtccaaact catcaatgta tcttatcatg tctggatccc catcaagctg atccggaacc
     3781 cttaatataa cttcgtataa tgtatgctat acgaagttat taggtccctc gacctgcagc
     3841 ccaagctaga tcgaattcgg ccggcccctt cacgtgttgc acttaacgcg tgccaccatg
     3901 acagagaccc tgcctcccgt gaccgagagt gccgtggccc ttcaagccga ggttacccaa
     3961 agggagttgt tcgagttcgt gctgaacgac cctttgcttg caagcagtct ctatatcaac
     4021 atcgcacttg caggactgag tatactgctg ttcgttttta tgacccgagg actcgatgat
     4081 ccacgggcaa aacttattgc tgtgtcaacc atccttgtgc ctgtcgtcag cattgcctcc
     4141 tacactggat tggcgagcgg cctgacaatt tccgttcttg aaatgccagc gggccatttt
     4201 gcagaaggca gctcagtgat gctgggagga gaagaggtag atggtgtagt caccatgtgg
     4261 ggacggtatc tcacctgggc actttccacg cccatgattc tcctcgctct gggtctcctg
     4321 gccggaagca atgctacaaa gctcttcaca gctatcactt tcgatatcgc tatgtgcgtg
     4381 actggccttg ccgcggccct gactacctcc tcccacctca tgagatggtt ctggtacgct
     4441 atcagttgtg catgctttct ggtggtcttg tatatcctgc tggtggagtg ggcacaggac
     4501 gccaaagccg cgggaaccgc tgacatgttc aataccctga agctgttgac agtagtgatg
     4561 tggctggggt atccaattgt gtgggctctt ggagtcgagg gtatcgcggt gttgcccgtt
     4621 ggggtgacga gctggggata ttctttcctg gatatcgtgg caaagtacat tttcgcattc
     4681 ttgctcctga actatctgac gtcaaacgaa tctgtcgtgt ccggcagcat tttggatgtt
     4741 ccatctgctt ctgggacccc ggctgatgat gcggccgcca agagcaggat caccagcgag
     4801 ggcgagtaca tccccctgga ccagatcgac atcaacgtgg tgagcaaggg cgaggagctg
     4861 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     4921 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     4981 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
     5041 ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     5101 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     5161 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     5221 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     5281 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     5341 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     5401 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
     5461 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     5521 gggatcactc tcggcatgga cgagctgtac aagttctgct acgagaacga ggtgtaatga
     5581 gaattcgacg cgtacaaggc cggccctgca ggaattcgat atcaagctta tcgataatca
     5641 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5701 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5761 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5821 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5881 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5941 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6001 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     6061 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6121 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6181 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     6241 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     6301 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     6361 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     6421 gattgggaag acaatagcag gcatgctggg gaactagtgg tgccagggcg tgcccttggg
     6481 ctccccgggc gcggcggccg catcgaattc taccgggtag gggaggcgct tttcccaagg
     6541 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     6601 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     6661 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     6721 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     6781 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     6841 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     6901 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     6961 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     7021 aatcgccgct agcgaagttc ctattctcta gaaagtatag gaacttcgcc accatgggat
     7081 cggccattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     7141 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     7201 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     7261 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     7321 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     7381 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     7441 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     7501 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     7561 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     7621 acggcgatga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     7681 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     7741 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     7801 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     7861 ttgacgagtt cttctgaggg gatccgctgt aagtctgcag aaattgatga tctattaaac
     7921 aataaagatg tccactaaaa tggaagtttt tcctgtcata ctttgttaag aagggtgaga
     7981 acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga
     8041 ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca
     8101 tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca
     8161 ctcatgatct atagatctat agatctctcg tgggatcatt gtttttctct tgattcccac
     8221 tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga
     8281 tcagcagcct ctgttccaca tacacttcat tctcagtatt gttttgccaa gttctaattc
     8341 catcagaaag cttgcagaag atctccccaa ctggggtaac ctttgagttc tctcagttgg
     8401 gggggcgcgc cctagaagat gggcgggagt cttctgggca ggcttaaagg ctaacctggt
     8461 gtgtgggcgt tgtcctgcag gggaattgaa caggtgtaaa attggaggga caagacttcc
     8521 cacagatttt cggttttgtc gggaagtttt ttaatagggg caaataggaa aatggaggat
     8581 aggagtcatc tggggtttat gcagcaaaac tacaggtata ttgcttgtat ccgcctcgga
     8641 gatttccatg aggagataaa gacatgtcac ccgagtttat actctcctgc ttagatccta
     8701 ctacagtatg aaatacagtg tygcgaggta gactatgtaa gcagatttaa tcattttaaa
     8761 gagcccagta cttcatatcc atttctcccg ctccttctgc agccttatca aaaggtattt
     8821 agaacactca ttttagcccc attttcattt attatactgg cttatccaac ccctagacag
     8881 agcattggca ttttcccttt cctgatctta gaagtctgat gactcatgaa accagacaga
     8941 ttagttacat acaccacaaa tcgaggctgt agctggggcc tcaacactgc agttctttta
     9001 taactcctta gtacactttt tgttgatcct ttgccttgat ccttaatttt cagtgtctat
     9061 cacctctccc gtcaggtggt gttccacatt tgggcctatt ctcagtccag ggagttttac
     9121 aacaatagat gtattgagaa tccaacctaa agcttaactt tccactccca tgaatgcctc
     9181 tctccttttt ctccattata actgagctat waccattaat ggtttcaggt ggatgtctcc
     9241 tcccccaata tacctgatgt atctacatat tgccaggctg atattttaag acatwaaagg
     9301 tatatttcat tattgagcca catggtattg attactgcta ctaaaatttt gtcattgtac
     9361 acatctgtaa aaggtggttc cttttggaat gcaaagttca ggtgtttgtt gtctttcctg
     9421 acctaaggtc ttgtgagctt gtattttttc tatttaagca gtgctttctc ttggactggc
     9481 ttgactcatg gcattctaca cgttattgct ggtctaaatg tgattttgcc aagcttcttc
     9541 aggacctata attttgcttg acttgtagcc aaacacaagt aaaatgatta agcaacaaat
     9601 gtatttgtga agcttggttt ttaggttgtt gtgttgtgtg tgcttgtgct ctataataat
     9661 actatccagg ggctggagag gtggctcgga gttcaagagc acagactgct cttccagaag
     9721 tcctgagttc aattcccagc aaccacatgg tggctcacaa ccatctgtaa tgggatctga
     9781 tgccctcttc tggtgtgtct gaagaccaca agtgtattca cattaaataa ataatcctcc
     9841 ttcttcttct tttttttttt ttaaagagaa twctgtctcc agtagaatta ctgaagtaat
     9901 gaaatacttt gtgtttgttc caatatggwa gccaataatc aaatactctt wagcactgga
     9961 aatgtaccaa ggaactattt tatttaagtg wactgtggac agaggagcca taactgcaga
    10021 cttgtgggat acagaagacc aatgcagact taatgtcttt tctcttacac taagcaataa
    10081 agaaataaaa attgaacttc tagtatccta tttgttaaac tgctagcttt actaactttt
    10141 gtgcttcatc tatacaaagc tgaaagctaa gtctgcagcc attactaaac atgaaagcaa
    10201 gtaatgataa ttttggattt caaaaatgta gggccagagt ttagccagcc agtggtggtg
    10261 cttgccttta tgccttaatc ccagcactct ggaggcagag acaggcagat ctctgagttt
    10321 gagcccagcc tggtctacac atcaagttct atctaggata gccaggaata cacacagaaa
    10381 ccctgttggg gaggggggct ctgagatttc ataaaattat aattgaagca ttccctaatg
    10441 agccactatg gatgtggcta aatccgtcta cctttctgat gagatttggg tattattttt
    10501 tctgtctctg ctgttggttg ggtcttttga cactgtgggc tttcttaaag cctccttccc
    10561 tgccatgtgg tctcttgttt gctactaact tcccatggct taaatggcat ggctttttgc
    10621 cttctaaggg cagctgctga gwtttgcagc ctgatttcca gggtggggtt gggaaatctt
    10681 tcaaacacta aaattgtcct ttaatttttt tttaaaaaat gggttatata ataaacctca
    10741 taaaatagtt atgaggagtg aggtggacta atattaatga gtccctcccc tataaaagag
    10801 ctattaaggc tttttgtctt atactaactt tttttttaaa tgtggtatct ttagaaccaa
    10861 gggtcttaga gttttagtat acagaaactg ttgcatcgct taatcagatt ttctagtttc
    10921 aaatccagag aatccaaatt cttcacagcc aaagtcaaat taagaatttc tgactttaat
    10981 gttatttgct actgtgaata taaaatgata gcttttcctg aggcagggtc tcactatgta
    11041 tctctgcctg atctgcaaca agatatgtag actaaagttc tgcctgcttt tgtctcctga
    11101 atactaaggt taaaatgtag taatactttt ggaacttgca ggtcagattc ttttataggg
    11161 gacacactaa gggagcttgg gtgatagttg gtaaatgtgt ttaagtgatg aaaacttgaa
    11221 ttattatcac cgcaacctac tttttaaaaa aaaaagccag gcctgttaga gcatgctaag
    11281 ggatccctag gacttgctga gcacacaaga gtagtacttg gcaggctcct ggtgagagca
    11341 tatttcaaaa aacaaggcag acaaccaaga aactacagta aggttacctg tctttaacca
    11401 tctgcatata cacagggata ttaaaatatt ccaaataata tttcattcaa gttttccccc
    11461 atcaaattgg gacatggatt tctccggtga ataggcagag ttggaaacta aacaaatgtt
    11521 ggttttgtga tttgtgaaat tgttttcaag tgatagttaa agcccatgag atacagaaca
    11581 aagctgctat ttcgaggtct cttggttata ctcagaagca cttctttggg tttccctgca
    11641 ctatcctgat catgtgctag gcctwcctta ggctgattgt tgttcaaata acttaagttt
    11701 cctgtcaggt gatgtcatat gatttcatat atcaaggcaa aacatgttat atatgttaaa
    11761 catttgkact taatgtgaaa gttaggtctt tgtgggtttt gattttaatt tcaaaacctg
    11821 agctaaataa gtcattttac atgtcttaca tttggtgaat tgtatattgt ggtttgcagg
    11881 caagactctc tgacctagta accctcctat agagcacttt gctgggtcac aagtctagga
    11941 gtcaagcatt tcaccttgaa gttgagacgt tttgttagtg tatactagtt atatgttgga
    12001 ggacatgttt atccagaaga tattcaggac tatttttgac tgggctaagg aattgattct
    12061 gattagcact gttagtgagc attgagtggc ctttaggctt gaattggagt cacttgtata
    12121 tctcaaataa tgctggcctt ttttwaaaag cccttgttct ttatcaccct gttttctaca
    12181 taatttttgt tcaaagaaat acttgtttgg atctcctttt gacaacaata gcatgttttc
    12241 aagccatatt ttttttcctt tttttttttt tttttggttt ttcgagacag ggtttctctg
    12301 tatagccctg gctgtcctgg aactcacttt gtagaccagg ctggcctcga actcagaaat
    12361 ccgcctgcct ctgcctcctg agtgccggga ttaaaggcgt gcaccaccac gcctggctaa
    12421 gttggatatt ttgtatataa ctataaccaa tactaactcc actgggtgga tttttaattc
    12481 agtcagtagt cttaagtggt ctttattggc ccttattaaa atctactgtt cactctaaca
    12541 gaggctgttg gactagtggs actaagcaac ttcctacgga tatactagca gataagggtc
    12601 agggatagaa actagtctag cgttttgtat acctaccagc ttatactacc ttgttctgat
    12661 agaaatattt aggacatcta gcttatcgat ccgtcgaggg ggggcccggt acccagcttt
    12721 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
    12781 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    12841 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    12901 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    12961 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    13021 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    13081 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    13141 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    13201 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    13261 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    13321 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    13381 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    13441 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    13501 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    13561 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
    13621 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    13681 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    13741 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    13801 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    13861 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    13921 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    13981 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    14041 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    14101 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    14161 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    14221 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    14281 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    14341 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    14401 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    14461 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    14521 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    14581 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    14641 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    14701 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    14761 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    14821 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    14881 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
    14941 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
    15001 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
    15061 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
    15121 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
    15181 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
    15241 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
    15301 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
    15361 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
    15421 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
    15481 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
    15541 aatacgactc actatagggc gaattggagc tcca
//
