LOCUS       Exported                 750 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    KHBD00007
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    54..78
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(511..610)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(627..646)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(628..646)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(651..667)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(651..667)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
       61 tgtacaaaaa agttggcacc atgaccgaac caaggacgcc cgagaagctg ctggccgcca
      121 agccgccggt tttacaccac aacagccata gtcccaatgc ctcgctgcag ctgcccagtc
      181 ccatcatcac gaggcgcact cgcacggcct cgacctccgc tcgggccttg gaaaatccgg
      241 ttgtgaccgg tatggtgaaa tccttcagcc gcaccaaagg acacggcttc attacaccca
      301 acgccggcgg agaggacgtt ttttgccacg tttccgacat cgaaggtgaa tatgtgccca
      361 tgcccgggga cgaggtcaaa taccgtctgt gcgccattcc gcccaagtat gagaagcacc
      421 aggccgtgca cgtgcagatt agccacctga cgccggaggt gcaccacaag tgggaggagc
      481 cattctatgg tggctcctcg cccgctaagt tgccaacttt cttgtacaaa gttggcatta
      541 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
      601 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
      661 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
      721 atatatcatc atgaacaata aaactgtctg
//
