LOCUS       Exported                 666 bp ds-DNA     linear   SYN 26-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pEntr-Tbx5flbio
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 666)
  AUTHORS   He A, Kong SW, Ma Q, Pu WT
  TITLE     Co-occupancy by multiple cardiac transcription factors identifies 
            transcriptional enhancers active in heart.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7.
  PUBMED    21415370
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 26, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    76..175
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(220..264)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(277..300)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acgggccaga
       61 gctgcagctg gatggcaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
      121 caaattgata agcaatgctt tcttataatg ccaactttgt acaagaaagc tgggtctaga
      181 tatctcgagt gcggccgcct acctcgagct cggcgcgcct tcgtgccatt cgattttctg
      241 agcctcgaag atgtcgttca ggccggaccc atcaccttta tcgtcatcgt ctttgtagtc
      301 acgcgtgcta ttgtcgctcc actctggcac catgccaact ccgtgcacag agtggtactg
      361 atgaggggat agagtccttg gcacgccatg agagtagagg aactcagggg gctgaagggt
      421 gccaggggac tgcaggccag tctgaggccc acactgcctg accacaggct ggtgggccac
      481 ggaggtctgg tgctggaaca ttccctctcc cagctgtggg gagccatggt tggccatgcc
      541 agccagccga gggaccaggg gccccgaggt gaagtgagcg gagaagtgct ggtagggtag
      601 cctgtccatg ggctgcacgg tggtgacggt gcagctgctg taggaaggca tgcttggcca
      661 cgtgtt
//
