LOCUS       Exported                 721 bp ds-DNA     linear   SYN 22-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    rAAV-syn::FLEX-rev::PSAML141F,Y115F:5HT3HC-IRES-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, Sternson SM
  TITLE     Chemical and genetic engineering of selective ion channel-ligand 
            interactions.
  JOURNAL   Science. 2011 Sep 2;333(6047):1292-6.
  PUBMED    21885782
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 22, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(40..73)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    complement(144..177)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
ORIGIN
        1 aattcgccct tttcagtgtt tcgaacctct tcgagggaca taacttcgta taatgtatgc
       61 tatacgaagt tatgggtcga tggtgagatc tggactagag ggtcgatggt gatgcttgga
      121 tcaaaatagg aagaatccca atgataactt cgtataaagt atcctatacg aagttatact
      181 agctagcaag cttgaattca ccatgcgctg ttctccaggc ggcgtgtggc tcgccctggc
      241 tgcttccctt ctgcacgtta gcctgcaggg tgagttccag cgcaaactgt ataaggagct
      301 tgttaagaat tataaccccc tggagcggcc ggtcgcaaat gattcccagc cactgacagt
      361 gtacttcagc ctctccttgc tgcagatcat ggacgtggat gaaaagaacc aggtgctgac
      421 cactaatatt tggttgcaga tgtcctggac cgatcactac ttgcagtgga atgtgagcga
      481 atacccaggt gtaaagactg taagattccc tgacggccaa atctggaaac cagatatcct
      541 gctgttcaac agcgcagacg aaaggtttga tgcaacattt cacaccaacg tgttggtcaa
      601 ttcttcaggc cactgccagt actttccccc tggaatcttc aagtcctcat gctatatcga
      661 cgtccgctgg tttcccttcg acgtccagca ctgcaaactc aaattcggga gctggagcta
      721 c
//
