LOCUS Exported 1247 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pCS2+8CeGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1247) AUTHORS Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A TITLE Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR) Efflux Transporters. JOURNAL J Biol Chem. 2012 Nov 2. PUBMED 23124201 REFERENCE 2 (bases 1 to 1247) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1247 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 219..237 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 219..236 /label=SP6 /note="SP6 promoter, forward primer" CDS 323..1039 /codon_start=1 /product="Aequoria victoria green fluorescent protein" /label=GFP /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" primer_bind complement(353..381) /label=GFP-R /note="GFP, reverse primer. Does NOT anneal to EGFP" primer_bind 976..996 /label=GFP-F /note="GFP, forward primer. Does NOT anneal to EGFP" promoter complement(1068..1085) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 1090..1224 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1114..1133) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1168..1187 /label=SV40pA-R /note="SV40 polyA, reverse primer" ORIGIN 1 agggtacatt ggcagtactc ccattgacgt caatggcggt aaatggcccg cgatggctgc 61 caagtacatc ccccattgac gtcaatgggg aggggcaatg acgcaaatgg gcgttccatt 121 gacgtaaatg ggcggtaggc gtgcctaatg ggaggtctat ataagcaatg ctcgtttagg 181 gaaccgccat tctgcctggg gacgtcggag caagcttgat ttaggtgaca ctatagaata 241 caagctactt gttctttttg caggatccac tagtggcgcg ccattaatta aaggccggcc 301 agcgatcgcc ggacatcgat tgatgagtaa aggagaagaa cttttcactg gagttgtccc 361 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg 421 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact 481 acctgttcca tggccaacac ttgtcactac tctcacttat ggtgttcaat gcttttcaag 541 atacccagat catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt 601 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa 661 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga 721 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat 781 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga 841 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt 901 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga 961 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat 1021 ggatgaacta tacaaataag aattcaaggc ctctcgagcc tctagaacta tagtgagtcg 1081 tattacgtag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga 1141 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc 1201 attataagct gcaataaaca agttaacaac aacaattgca ttcattt //